Carriage of Haemophilus influenzae in the oropharynx of young children and molecular epidemiology of the isolates after fifteen years of H. influenzae type b vaccination in Italy

Giufrè, Maria; Daprai, Laura; Cardines, Rita; Bernaschi, Paola; Ravà, Lucilla; Accogli, Marisa; Raponi, Massimiliano; Garlaschi, M.L.; Atti, Marta Luisa Ciofi degli; Cerquetti, Marina

doi:10.1016/j.vaccine.2015.09.082

Cited by 49 publications

(41 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…On the other hand, ceftriaxone, a third-generation molecule (Figure 2b), having the metoxyimino group and the aminothiazole substitution in the C7 side chain, has a good β-lactamase stability and a higher activity on Gram-negative bacteria, PBP 3 protein, caused recent concern for its increasing occurrence in several European countries. [24][25][26] When treating AECB, to prevent the intra-species diffusion of BLNAR and BLPACR resistant strains it is mandatory using antibiotics able to target also the emergent strains. The expert panel reached consensus (78%) also on this statement ( Table 3).…”

Section: Resultsmentioning

confidence: 99%

The most appropriate therapeutic strategy for acute lower respiratory tract infections: a Delphi-based approach

Blasi

Concia

Prato

et al. 2017

Journal of Chemotherapy

View full text Add to dashboard Cite

Lower respiratory tract infections (LRTIs) cause high morbidity and mortality worldwide. Empiric therapy often base the choice of antibiotic treatment on antibacterial spectrum of the agent rather than on its pharmacological properties or the pathogen resistance profile. Inappropriate prescribing leads to therapeutic failure and antibiotic resistance, with increasing direct and indirect health costs. A consensus on appropriate prescribing in LRTI therapy was appraised by this Delphi exercise, based on a panel of 70 pulmonologists, coordinated by a Scientific Committee of nine experts in respiratory medical care. Full or very high consensus was reached on several issues, including the role of oral cephalosporins in first-line treatments of LRTIs and the appropriateness of cefditoren, with balanced spectrum and high intrinsic activity, in LRTI treatment. Evidence-based medicine approach and a comprehensive process of disease management, from diagnosis to therapy and follow-up, should guide antibiotic prescribing.

show abstract

Section: Resultsmentioning

confidence: 99%

The most appropriate therapeutic strategy for acute lower respiratory tract infections: a Delphi-based approach

Blasi

Concia

Prato

et al. 2017

Journal of Chemotherapy

View full text Add to dashboard Cite

show abstract

“…A group of 107/1025 (10.4%) isolates was thus recognized by protein profile as other Haemophilus spp., with a score <2.000. 15.3%, respectively). Still, H. influenzae isolates were more common whether they were taken from children, both healthy and unhealthy, in comparison to those taken from adult patients.…”

Section: Haemophili Isolates Identificationmentioning

confidence: 86%

“…This mechanism results in a resistance to aminopenicillins (ampicillin, amoxicillin) without a loss of the sensitivity for combination inhibitor therapy (amoxicillin-clavulanic acid) [10,11]. The prevalence of beta-lactamase-positive strains isolated from respiratory tract infections and asymptomatic colonizers vary from a few to several tens of percent in the various European countries [10,11,15,48].…”

Section: Discussionmentioning

confidence: 99%

“…These include the pathogenic H. influenzae species. Children under six months of age rarely are the carriers of these bacteria, because of the presence of maternal antibodies protecting against them [10,15,40,48]. However, such passive immunity obtained from the mother's milk does not last long, and 90% of all children >1 year of age are carriers of these bacteria.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Phenotypic diversity of Haemophilus influenzae and Haemophilus parainfluenzae isolates depending on origin and health condition

Andrzejczuk

Kosikowska

Malm

et al. 2017

Current Issues in Pharmacy and Medical Sciences

View full text Add to dashboard Cite

Background. Haemophili are common human microbiota representatives. The aim of our study was to investigate a diversity of Haemophilus spp. isolates selected from clinical specimens on the basis of biochemical characteristics, biotypes distribution, protein profiles and antimicrobial resistance. Results. A total of 893/1025 (87%) of haemophili isolates were identified: 260/1025 (25%) as H. influenzae and 633/1025 (62%) as H. parainfluenzae. Moreover, a group of 107/1025 (10%) isolates without species identification (with e.g. abnormal numerical profile) was described as Haemophilus spp. Within the H. influenzae isolates, biotypes II and III were in a great majority (92/893; 10%, each), whereas among H. parainfluenzae, the most commonly occurring was biotype I and II (301/893, 34% and 178/893, 20%, respectively). A similar prevalence of biotypes was obtained regardless of the patient’s age or health condition or the type of specimen. A production of beta-lactamases was shown in 46/893 (5%) haemophili, both H. influenzae (13/46, 28%) and H. parainfluenzae (33/46, 72%) isolates. On the basis of haemophili biochemical characteristics, the cluster analysis using the UPGMA method demonstrated a high degree of phenotypic similarity due to a small distances between isolates taken from both unhealthy children and adults. Conclusion. Based on biochemical characteristics, about 90% of haemophili clinical isolates representing human-specific respiratory microbiota were positively identified as H. influenzae and H. parainfluenzae. The same differences in biotypes and antimicrobial resistance among isolates selected from healthy people or from patients with chronic and recurrent diseases were detected.

show abstract

“…20 In the present post-Hib vaccination era, NTHi strongly predominates among H. influenzae isolates in the oropharynx and nasopharynx of healthy children with carriage rates varying from 10% to more than 60%, depending on the children age. 21,22,23,24 Along with H. influenzae, S. pneumoniae colonizes the upper respiratory tracts of children and is responsible for mild or severe diseases.…”

Section: Impact Of the Conjugate Vaccines Against Both Hib And Streptmentioning

confidence: 99%

Why we need a vaccine for non-typeable Haemophilus influenzae

Cerquetti

Giufrè

2016

Human Vaccines & Immunotherapeutics

Self Cite

View full text Add to dashboard Cite

Carriage of Haemophilus influenzae in the oropharynx of young children and molecular epidemiology of the isolates after fifteen years of H. influenzae type b vaccination in Italy

Cited by 49 publications

References 38 publications

The most appropriate therapeutic strategy for acute lower respiratory tract infections: a Delphi-based approach

The most appropriate therapeutic strategy for acute lower respiratory tract infections: a Delphi-based approach

Phenotypic diversity of Haemophilus influenzae and Haemophilus parainfluenzae isolates depending on origin and health condition

Why we need a vaccine for non-typeable Haemophilus influenzae

Contact Info

Product

Resources

About