Carrier-free catalyzed hairpin assembly propelled sensing-to-therapy DNA amplifier under a cascaded light-responsive switching

Sensitive fluorescence assay of chloramphenicol coupled with two-level isothermal amplification using a self-powered catalyzed hairpin assembly and entropy-driven circuit

“…28 So far, they have been widely used for the bio-sensing of various targets such as ions, small molecules, DNA, RNA, proteins and cells. [28][29][30][31] However, coupling two levels of isothermal amplication of nucleic acids in one strategy normally requires a long reaction time (more than 4 h) due to the low amplication efficiency; 32,33 the long incubation time limits its practical application.…”

pH-Stimulated Self-Locked DNA Nanostructure for the Effective Discrimination of Cancer Cells and Simultaneous Detection and Imaging of Endogenous Dual-MicroRNAs

“…MicroRNAs (miRNA) are endogenous small single-stranded noncoding RNAs that have been applied as biomarkers in the field of oncology. − Usually, fluorescence microscopy-based molecular imaging techniques have been extensively developed as standard methods for the live-cell imaging of intracellular miRNAs. − Although current live-cell imaging methods have improved the sensitivity of miRNA detection, − there is still a significant drawback: cancer cells cannot be completely distinguished from normal cells, which could cause false diagnosis and delayed treatment. Therefore, a novel strategy is desperately needed to not only distinguish cancer cells from normal cells at the molecular level but also detect the miRNAs in vitro sensitively and achieve high-resolution imaging of miRNAs in live cancer cells.…”