Selective excitation of tryptophan fluorescence decay in proteins using a subnanosecond 295 nm light-emitting diode and time-correlated single-photon counting Appl. Phys. Lett. 86, 261911 (2005); 10.1063/1.1984088 Ultraviolet light-emitting diodes operating in the 340 nm wavelength range and application to time-resolved fluorescence spectroscopyWe have produced amplitude-modulated near-ultraviolet light, centered at 390 nm, using an inexpensive, commercially available blue light-emitting diode ͑LED͒. The LED was amplitude modulated with the ϩ13 dBm ac output from a frequency generator while biased through a bias tee with 60 mA of dc current. The LED produced 45 to 54 W of UV light over the modulation bandwidth of 0.01 to 200 MHz, when measured after optical filters to remove the residual blue output. Since the filter attenuated the UV output about 3 dB, more than 100 W of UV light was initially produced. Modulated UV light was available to approximately 200 MHz, with a Ϫ3 dB point of 31 MHz, allowing the measurement of ns fluorescence lifetimes. The fluorescence lifetimes of standard fluorophores ͑9-cyanoanthracene and green fluorescent protein͒ were measured in the frequency domain using the phase-modulation technique, producing lifetimes that closely agree with those reported in the literature, confirming that the UV-emitting blue LED is practical for spectroscopic and sensor applications. When compared to a laser modulated with a Pockels cell, the LED was smaller, less expensive, required less power, generated less heat, and required less alignment. The ability to modulate the LED at high frequencies, along with the UV output, allows its use as an inexpensive UV light source in fluorescence lifetime optical sensors and even frequency-domain fluorometers.