“…The CCS uses a circular DNA template by ligating hairpin adaptors to both ends of target double-stranded DNA, so the DNA template is sequenced multiple times to generate a continuous long read (Weirather et al, 2017). Nanopore sequencing, uses nanopores to sequence native single-stranded DNA, by measuring the changes in an electric current passed across the pore as the DNA bases pass through, disrupting the current to different levels with different nucleotides (Giordano et al, 2017).…”