CART peptide in the nucleus accumbens shell acts downstream to dopamine and mediates the reward and reinforcement actions of morphine

Upadhya, Manoj A.; Nakhate, Kartik T.; Kokare, Dadasaheb M.; Singh, Uday; Singru, Praful S.; Subhedar, Nishikant K.

doi:10.1016/j.neuropharm.2011.12.004

Cited by 50 publications

(35 citation statements)

References 66 publications

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“…Similar effect was also noted under standard chow or a nutritionally complete liquid diet, in either normal or diet-induced obese animals [review, 15]]. On the other hand, bilateral injection of CART in the AcbSh significantly increased pellet self-administration which suggested that CART promotes hedonic component of food intake [16]. In view of the functional duality associated with CART, we hypothesize its role in reward based feeding, a characteristic feature of BE.…”

Section: Introductionsupporting

confidence: 68%

“…h-003-62) for 24-h at 4 • C following washing with PBS, treating with 0.5% TritonX-100 for 20-min and incubation in 5% bovine serum albumin (BSA) for 60-min. Thereafter, the sections were washed in PBS and incubated in Alexa Fluor ® 488-conjugated anti-rabbit IgG (Jackson Immunoresearch, West Groove, PA, USA) at 1:500 dilution for 3-h at room temperature [16].…”

Section: Immunohistochemistrymentioning

confidence: 99%

“…Rats (N = 3) were implanted with cannula targeted at the right AcbSh using the stereotaxic coordinates (+0.7 mm anterior, +1.0 mm right to midline and 6.5 mm ventral) with respect to bregma [16,28]. Following 7 days of recovery, 0.25 l of Di-I (1%) was injected in AcbSh.…”

Section: Di-i Retrograde Neuronal Tracingmentioning

confidence: 99%

“…Brain derived neurotrophic factor (BDNF) is known to induce anorexic as well as reward-like behavior [13]. On similar lines, cocaine-and amphetamine-regulated transcript peptide (CART) has emerged as an important neuropeptide of hypothalamic feeding circuitry that mediates anorexia and contributes to food reward [14][15][16]. In 1998, Kristensen et al demonstrated that intracerebroventricular (icv) injection of recombinant CART (55-102) dose dependently inhibited feeding in normal and fasted rats, and suppressed the normal feeding response to neuropeptide Y [14].…”

Section: Introductionmentioning

confidence: 99%

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Differential expression of CART in feeding and reward circuits in binge eating rat model

Bharne

Borkar

Subhedar

et al. 2015

Behavioural Brain Research

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Section: Introductionsupporting

confidence: 68%

Section: Immunohistochemistrymentioning

confidence: 99%

Section: Di-i Retrograde Neuronal Tracingmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Differential expression of CART in feeding and reward circuits in binge eating rat model

Bharne

Borkar

Subhedar

et al. 2015

Behavioural Brain Research

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“…among these regions, alteration of CaRt expression during aging has been shown only in the arcuate nucleus [34]. CaRt-immunopositive neuronal somata in the naCC are densely innervated by dopaminergic axon terminals mostly coming from the Vta, another brain area that regulates feeding behavior [27,30]. Concerning the dopamine signal in the naCC of elderly animals, decreased binding to dopaminergic receptors and lower basal extracellular dopamine release were measured indicating age-related change in the VTA [9,36].…”

Section: The Ages Of the Animalsmentioning

confidence: 99%

Stability of CART peptide expression in the nucleus accumbens in aging

Armbruszt¹,

Figler²,

Ábrahám³

2015

Acta Biologica Hungarica

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aging is accompanied by changes of several anorexigenic and orexigenic neuropeptides expressed in various brain areas that control food intake and these changes correlate with senescent anorexia. during aging expression of cocaine-and amphetamine-regulated transcript (CaRt) peptide was reported to be reduced in the hypothalamic nuclei related to food intake. although CaRt peptide is abundant in the nucleus accumbens that also plays a crucial role in the food intake regulation, no data is available about the CaRt peptide expression in this region through aging. in the present study, CaRt peptide immunoreactivity was compared in the nucleus accumbens of young adult (4-and 7-month-old) middle-aged (15-month-old) and aging (25-32-month-old) long-evans rats. the density of CaRt-immunoreactive cells and axon terminals in the nucleus accumbens was measured with computer-aided densitometry. CART-immunodensity was similar in the old rats and in the younger animals without significant difference between age groups. in addition, no gender-difference was observed when CaRt-immunoreactivities in the nucleus accumbens of male and female animals were compared. our results indicate that CaRt peptide expression in the nucleus accumbens is stable in adults and does not change with age.

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Pro‐cognitive action of CART is mediated via ERK in the hippocampus

et al. 2016

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Although cocaine- and amphetamine-regulated transcript peptide (CART) is detected in several cortical and subcortical areas, its role in higher functions has been largely ignored. We examined the significance of CART in memory formation and tested if the downstream actions of CART involve N-methyl-d-aspartate (NMDA) activated extra-cellular signal-regulated kinase (ERK). Newly formed memory was evaluated using novel object recognition test consisting of familiarization (T1) and choice trials (T2). The choice trials were performed at two time points: 30-min (T230-min ) and 24-h (T224-h ) postacquisition. In choice trial (T230-min ), vehicle control rats explored the novel object for significantly longer duration than the familiar object indicating intact memory formation. However, CART-antibody, U0126 [ERK antagonist, both via intracerebroventricular (icv) or intrahippocampal (ih) route] or MK-801 (NMDA antagonist; intraperitoneal) treated rats spent less time exploring novel objects; CART peptide (icv or ih) was ineffective. During choice trial at T224-h , a significant decrease in novel object exploration time was noticed in vehicle control rats suggesting amnesia. However, treatment with CART, prior to familiarization trial (T1), promoted exploration of the novel object even at T224-h . Pretreatment with U0126 or MK-801 blocked pro-cognitive-like effect of CART suggesting involvement of NMDA-ERK pathway in CART's action. Animals subjected to the object familiarization trial showed a drastic increase in the CART-immunoreactivity in the cells of cornu ammonis 3 and polymorph layer of dentate gyrus, and fibers within ento- (ENT) and peri-rhinal (PRH) cortices. Western blot analysis revealed that CART treatment significantly up-regulated the expression of phospo-ERK1/2 in hippocampus, ENT and PRH. This effect was attenuated following pretreatment with U0126 or MK-801, suggesting the activation of ERK signaling cascade through NMDA receptors. Thus, CART system seems to play an important role in recognition memory and that these effects may be mediated by NMDA receptors-ERK signaling in the ENT/PRH-hippocampal circuit. © 2016 Wiley Periodicals, Inc.

show abstract

CART peptide in the nucleus accumbens shell acts downstream to dopamine and mediates the reward and reinforcement actions of morphine

Cited by 50 publications

References 66 publications

Differential expression of CART in feeding and reward circuits in binge eating rat model

Differential expression of CART in feeding and reward circuits in binge eating rat model

Stability of CART peptide expression in the nucleus accumbens in aging

Pro‐cognitive action of CART is mediated via ERK in the hippocampus

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