Carthamidin-mediated silver nanoparticles synthesis, characterization and anticancer activity-Invitro Approach

Abstract

The synthesis of silver nanoparticles from biological sources is been fascinated by the research fraternities owing to the distinctive feature of non-toxicity and benign synthesis methodologies. In this pipeline, we have synthesized silver nanoparticles (AgNPs) from flavonoid Carthamidin (CT) pigment. The yellow colored water soluble pigment reduces the silver nitrate into silver ions in a simple one pot method. The CTAgNPs were exclusively characterized by UV-Vis spectroscopy, X-ray Diffraction (XRD), Transmision Electron Microscopy (TEM), Scanning Electron Microscopy (SEM) and Energy Dispersive Analysis Spectroscopy (EDAX). The characterization techniques infer that CTAgNPs are spherical in shape with an average size of 59nm and face centered cubic with crystalline lattice. The CTAgNPs evaluated as a cytotoxic agent in the MCF 7 cells and molecularly annotated the CTAgNPs apoptosis induction by AO/EB staining, Reactive Oxygen Species (ROS) assay, quantification of lactate Dehydrogenase (LDH) and arresting of cell cycle by flow cytometry. Finally, the CTAgNPs possess a remarkable cytotoxicity in the MCF 7 cells and induce the apoptosis mechanism. Henceforth, CTAgNPs can be promulgated as a nanodrug for the treatment of breast cancer therapy.