Cartilage-derived morphogenetic protein-1 promotes the differentiation of mesenchymal stem cells into chondrocytes

Bai, Xiaowen; Xiao, Zhifeng; Pan, Yuqiong; Hu, Jiang; Pohl, J; Wen, Jinhua; Li, Lingsong

doi:10.1016/j.bbrc.2004.10.055

Cited by 82 publications

(74 citation statements)

References 43 publications

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“…On the other hand, these data may also imply that exogenous rhGDF-5 treatment heals defects via classical endochondral ossification, while the defects enhanced with rhBMP-2 may heal through intramembranous ossification. In fact, these observations are supported by a number of reports in the literature where it is demonstrated that bone repairs with exogenous rhGDF-5 treatment initiated endochondral ossification (Erlacher et al 1998b;Bai et al 2004;Kakudo et al 2007;Kadomatsu et al 2008). At the same time, other studies from the literature have reported that chondrogenic and osteogenic lineage pathways in response to rhGDF-5 treatment are dose and carrier dependent (Spiro et .…”

Section: Discussionsupporting

confidence: 56%

Time kinetics of bone defect healing in response to BMP-2 and GDF-5 characterised by in vivo biomechanics

Wulsten

Glatt

Ellinghaus

et al. 2011

eCM

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This study reports that treatment of osseous defects with different growth factors initiates distinct rates of repair. We developed a new method for monitoring the progression of repair, based upon measuring the in vivo mechanical properties of healing bone. Two different members of the bone morphogenetic protein (BMP) family were chosen to initiate defect healing: BMP-2 to induce osteogenesis, and growth-and-differentiation factor (GDF)-5 to induce chondrogenesis. To evaluate bone healing, BMPs were implanted into stabilised 5 mm bone defects in rat femurs and compared to controls. During the first two weeks, in vivo biomechanical measurements showed similar values regardless of the treatment used. However, 2 weeks after surgery, the rhBMP-2 group had a substantial increase in stiffness, which was supported by the imaging modalities. Although the rhGDF-5 group showed comparable mechanical properties at 6 weeks as the rhBMP-2 group, the temporal development of regenerating tissues appeared different with rhGDF-5, resulting in a smaller callus and delayed tissue mineralisation. Moreover, histology showed the presence of cartilage in the rhGDF-5 group whereas the rhBMP-2 group had no cartilaginous tissue.Therefore, this study shows that rhBMP-2 and rhGDF-5 treated defects, under the same conditions, use distinct rates of bone healing as shown by the tissue mechanical properties. Furthermore, results showed that in vivo biomechanical method is capable of detecting differences in healing rate by means of change in callus stiffness due to tissue mineralisation.

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Section: Discussionsupporting

confidence: 56%

Time kinetics of bone defect healing in response to BMP-2 and GDF-5 characterised by in vivo biomechanics

Wulsten

Glatt

Ellinghaus

et al. 2011

eCM

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“…For chondrocytic differentiation in vitro of MSCs, it had been demonstrated that TGF-β was necessary for induction in vitro (Worster et al, 2001;Indrawattana et al, 2004;Lisignoli et al, 2005;Bai et al, 2004;Lee et al, 2004;Chen et al, 2003). In addition, other inducers were supplemented for chondrogenic induction, such as CDMP-1 (Bai et al, 2004), BMP (Indrawattana et al, 2004;Lee et al, 2004), GDF-5 (Lee et al, 2004), IGF-I (Worster et al, 2001;Zhang et al, 2004a;Indrawattana et al, 2004) or dexamethasone (Mackay et al, 1998). TGF-β induced-chondrogenesis was regulated through ERK1/2 signaling pathway.…”

Section: Discussionmentioning

confidence: 99%

Ex vivo expansion and pluripotential differentiation of cryopreserved human bone marrow mesenchymal stem cells

Xiang

Zheng

Jia

et al. 2007

J. Zhejiang Univ. - Sci. B

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This study is aimed at investigating the potentials of ex vivo expansion and pluri-differentiation of cryopreservation of adult human bone marrow mesenchymal stem cells (hMSCs) into chondrocytes, adipocytes and neurocytes. Cryopreserved hMSCs were resuscitated and cultured for 15 passages, and then induced into chondrocytes, adipocytes and neurocytes with corresponding induction medium. The induced cells were observed for morphological properties and detected for expressions of type II collagen, triglyceride or neuron-specific enolase and nestin. The result showed that the resuscitated cells could differentiate into chondrocytes after exposure to transforming growth factor β 1 (TGF-β 1 ), insulin-like growth factor I (IGF-I) and vitamin C (V C ), and uniformly changed morphologically from a spindle-like fibroblastic appearance to a polygonal shape in three weeks. The induced cells were heterochromatic to safranin O and expressed cartilage matrix-procollagenal (II) mRNA. The resuscitated cells cultured in induction medium consisting of dexamethasone, 3-isobutyl-1-methylxanthine, indomethacin and IGF-I showed adipogenesis, and lipid vacuoles accumulation was detectable after 21 d. The resuscitated hMSCs were also induced into neurocytes and expressed nestin and neuron specific endolase (NSE) that were special surface markers associated with neural cells at different stage. This study suggested that the resuscitated hMSCs should be still a population of pluripotential cells and that it could be used for establishing an abundant hMSC reservoir for further experiment and treatment of various clinical diseases.

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“…However, during differentiation process, collagen IIB, an adult form was detected in as early as day 3 embryoid bodies of mES-hSOX9 cells. Extensive studies on in vitro chondrogenic differentiations and the molecular mechanisms have been performed in mesenchymal cells (Ahrens et al, 1993;Erlebacher et al, 1995;Mackay et al, 1998;Bai et al, 2004;Ikeda et al, 2004). Recently, it has been reported that even primary mouse embryonic fibroblasts can enter and complete the program of chondrogenic differentiation under the appropriate conditions (Lengner et al, 2004).…”

Section: Discussionmentioning

confidence: 99%

Overexpression of SOX9 in mouse embryonic stem cells directs the immediate chondrogenic commitment

Kim

Do²,

Yang³

et al. 2005

Exp Mol Med

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Cartilage-derived morphogenetic protein-1 promotes the differentiation of mesenchymal stem cells into chondrocytes

Cited by 82 publications

References 43 publications

Time kinetics of bone defect healing in response to BMP-2 and GDF-5 characterised by in vivo biomechanics

Time kinetics of bone defect healing in response to BMP-2 and GDF-5 characterised by in vivo biomechanics

Ex vivo expansion and pluripotential differentiation of cryopreserved human bone marrow mesenchymal stem cells

Overexpression of SOX9 in mouse embryonic stem cells directs the immediate chondrogenic commitment

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