Cartilage Derived Morphogenetic Protein-2 Induces Cell Migration and Its Chondrogenic Potential in C28/I2 Cells

Zhou, Frank Y.; Wei, Ai-Qun; Shen, Bo; Williams, Lisa; Diwan, Ashish D.

doi:10.14444/2052

Cited by 5 publications

(5 citation statements)

References 43 publications

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“…Interestingly, at high concentrations (800 ng/mL) GDF6 was also shown to act as a potent chemoattractant for NP cells with similar responses observed to potent chemoattractants, such as fetal calf serum (FCS) . This result supports evidence that GDF6 can also act as a chemoattractant to chondrocytes . In a recent RNA sequencing study investigating regulatory networks controlling ECM synthesis in the human, IVD, GDF6, and GDF5 were identified as important human NP (but not AF) coregulatory gene networks .…”

Section: The Effect Of Supplementation With Gdfs On Disc Cells and Chmentioning

confidence: 52%

“…In a recent RNA sequencing study investigating regulatory networks controlling ECM synthesis in the human, IVD, GDF6, and GDF5 were identified as important human NP (but not AF) coregulatory gene networks . GDF6 supplementation also induces aggrecan expression and PG production in articular chondrocytes and human chondrocyte cell line C28/I2 …”

Section: The Effect Of Supplementation With Gdfs On Disc Cells and Chmentioning

confidence: 99%

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Therapeutic potential of growth differentiation factors in the treatment of degenerative disc diseases

et al. 2019

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Intervertebral disc (IVD) degeneration is a major contributing factor to chronic low back pain and disability, leading to imbalance between anabolic and catabolic processes, altered extracellular matrix composition, loss of tissue hydration, inflammation, and impaired mechanical functionality. Current treatments aim to manage symptoms rather than treat underlying pathology. Therefore, IVD degeneration is a target for regenerative medicine strategies. Research has focused on understanding the molecular process of degeneration and the identification of various factors that may have the ability to halt and even reverse the degenerative process. One such family of growth factors, the growth differentiation factor (GDF) family, have shown particular promise for disc regeneration in in vitro and in vivo models of IVD degeneration. This review outlines our current understanding of IVD degeneration, and in this context, aims to discuss recent advancements in the use of GDF family members as anabolic factors for disc regeneration. An increasing body of evidence indicates that GDF family members are central to IVD homeostatic processes and are able to upregulate healthy nucleus pulposus cell marker genes in degenerative cells, induce mesenchymal stem cells to differentiate into nucleus pulposus cells and even act as chemotactic signals mobilizing resident cell populations during disc injury repair. The understanding of GDF signaling and its interplay with inflammatory and catabolic processes may be critical for the future development of effective IVD regeneration therapies.

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Section: The Effect Of Supplementation With Gdfs On Disc Cells and Chmentioning

confidence: 52%

Section: The Effect Of Supplementation With Gdfs On Disc Cells and Chmentioning

confidence: 99%

Therapeutic potential of growth differentiation factors in the treatment of degenerative disc diseases

et al. 2019

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“…Gene therapies or the addition of specific bone morphogenetic proteins were shown to accelerate the regeneration process, stimulate ECM production, or prevent the progression of annular injuries [ 90 , 91 , 92 , 93 , 94 ]. However, the delivery of genes into the AF cells has been technically challenging due to the low population of cells.…”

Section: Regenerative Strategiesmentioning

confidence: 99%

“…The effect of growth differentiation factors (GDFs) to enhance the anabolism-catabolism ratio in the ECM has been considered as an effective biomolecule-based regenerative strategy [ 93 , 97 ]. An increasing body of evidence indicated that GDFs were central to the IVD homeostatic processes, upregulation of the healthy cell marker genes in degenerative cells, and differentiation of mesenchymal stem cells to the NP cells [ 98 , 99 , 100 ].…”

Section: Regenerative Strategiesmentioning

confidence: 99%

“…An increasing body of evidence indicated that GDFs were central to the IVD homeostatic processes, upregulation of the healthy cell marker genes in degenerative cells, and differentiation of mesenchymal stem cells to the NP cells [ 98 , 99 , 100 ]. Of particular significance, the efficacy of recombinant protein GDF6 has been shown to enhance stem cells’ immobilization leading to healing of the AF and regenerating the NP in ovine and lapin models and improved pain in a rat-allograft model [ 92 , 93 , 101 , 102 ]. This paracrine function of GDFs’ may off set the short lifetime in vivo which has been considered a limitation restraining their efficacy to regenerate the AF [ 103 ].…”

Section: Regenerative Strategiesmentioning

confidence: 99%

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Advanced Strategies for the Regeneration of Lumbar Disc Annulus Fibrosus

Tavakoli

Diwan

Tipper

2020

IJMS

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Damage to the annulus fibrosus (AF), the outer region of the intervertebral disc (IVD), results in an undesirable condition that may accelerate IVD degeneration causing low back pain. Despite intense research interest, attempts to regenerate the IVD have failed so far and no effective strategy has translated into a successful clinical outcome. Of particular significance, the failure of strategies to repair the AF has been a major drawback in the regeneration of IVD and nucleus replacement. It is unlikely to secure regenerative mediators (cells, genes, and biomolecules) and artificial nucleus materials after injection with an unsealed AF, as IVD is exposed to significant load and large deformation during daily activities. The AF defects strongly change the mechanical properties of the IVD and activate catabolic routes that are responsible for accelerating IVD degeneration. Therefore, there is a strong need to develop effective therapeutic strategies to prevent or reconstruct AF damage to support operational IVD regenerative strategies and nucleus replacement. By the way of this review, repair and regenerative strategies for AF reconstruction, their current status, challenges ahead, and future outlooks were discussed.

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Mechano-active materials for musculoskeletal tissue engineering

Lin

et al. 2022

Musculoskeletal Tissue Engineering

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Cartilage Derived Morphogenetic Protein-2 Induces Cell Migration and Its Chondrogenic Potential in C28/I2 Cells

Cited by 5 publications

References 43 publications

Therapeutic potential of growth differentiation factors in the treatment of degenerative disc diseases

Therapeutic potential of growth differentiation factors in the treatment of degenerative disc diseases

Advanced Strategies for the Regeneration of Lumbar Disc Annulus Fibrosus

Mechano-active materials for musculoskeletal tissue engineering

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