Cartilage Tissue Engineering by the 3D Bioprinting of iPS Cells in a Nanocellulose/Alginate Bioink

Nguyen, Duong Thanh; Hägg, Daniel; Forsman, Alma; Ekholm, Josefine; Nimkingratana, Puwapong; Brantsing, Camilla; Kalogeropoulos, T.; Zaunz, Samantha; Concaro, Sebastian; Brittberg, Mats; Lindahl, Anders; Gatenholm, Paul; Enejder, Annika; Simonsson, Stina

doi:10.1038/s41598-017-00690-y

Cited by 378 publications

(343 citation statements)

References 35 publications

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“…Gel‐like materials are readily printed using pressure‐driven printers, also known as bioprinters. Alginate and CNF mixtures have already been demonstrated as being suitable for 3D printing, as the ratio of alginate to CNF and the ionic strength give a precise control over the viscosity of the gel, making it possible to print thin and stable gel layers. As shown in Figure , a 3D‐printing step can be implemented in the aerogel preparation route.…”

Section: Aerogels From Cellulose Nanofibers and Sodium Alginatementioning

confidence: 99%

Ambient‐Dried, 3D‐Printable and Electrically Conducting Cellulose Nanofiber Aerogels by Inclusion of Functional Polymers

Françon

Wang

Marais

et al. 2020

Adv Funct Materials

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112

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This study presents a novel, green, and efficient way of preparing crosslinked aerogels from cellulose nanofibers (CNFs) and alginate using non‐covalent chemistry. This new process can ultimately facilitate the fast, continuous, and large‐scale production of porous, light‐weight materials as it does not require freeze‐drying, supercritical CO2 drying, or any environmentally harmful crosslinking chemistries. The reported preparation procedure relies solely on the successive freezing, solvent‐exchange, and ambient drying of composite CNF‐alginate gels. The presented findings suggest that a highly‐porous structure can be preserved throughout the process by simply controlling the ionic strength of the gel. Aerogels with tunable densities (23–38 kg m−3) and compressive moduli (97–275 kPa) can be prepared by using different CNF concentrations. These low‐density networks have a unique combination of formability (using molding or 3D‐printing) and wet‐stability (when ion exchanged to calcium ions). To demonstrate their use in advanced wet applications, the printed aerogels are functionalized with very high loadings of conducting poly(3,4‐ethylenedioxythiophene):tosylate (PEDOT:TOS) polymer by using a novel in situ polymerization approach. In‐depth material characterization reveals that these aerogels have the potential to be used in not only energy storage applications (specific capacitance of 78 F g−1), but also as mechanical‐strain and humidity sensors.

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Section: Aerogels From Cellulose Nanofibers and Sodium Alginatementioning

confidence: 99%

Ambient‐Dried, 3D‐Printable and Electrically Conducting Cellulose Nanofiber Aerogels by Inclusion of Functional Polymers

Françon

Wang

Marais

et al. 2020

Adv Funct Materials

Self Cite

112

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“…Nguyen et al bioprinted iPSCs combined with irradiated chondrocytes and hyaline cartilage tissue which formed hyaline like cartilage with type II collagen expression (Fig. A) . Recently, another group compared three different bioinks loaded with BMSCs (a) GelMA, (b) GelMA + chondroitin sulfate aminoethyl methacrylate (CS‐AEMA), and (c) GelMA + CS‐AEMA + hyaluronic acid methacrylate .…”

Section: Bioprinted Tissuesmentioning

confidence: 99%

“… Bioprinted tissues. (A) : 3D‐bioprinted chondrocyte‐derived iPSCs at week 5 of differentiation, sections stained for GAGs, Safranin O for cartilage (with nuclear counterstain), and H&E for extracellular matrix (with nuclear counterstain) (the scale bar represents 100 μm or 500 μm) (Reproduced with permission from ). (B) : Micro‐CT images of polylactic acid/hydroxyapatite scaffold (left) versus bone defect without scaffold (right) after 4 weeks in vivo.…”

Section: Bioprinted Tissuesmentioning

confidence: 99%

Concise Review: Bioprinting of Stem Cells for Transplantable Tissue Fabrication

Leberfinger

Ravnic

Dhawan

et al. 2017

Stem Cells Translational Medicine

146

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Bioprinting is a quickly progressing technology, which holds the potential to generate replacement tissues and organs. Stem cells offer several advantages over differentiated cells for use as starting materials, including the potential for autologous tissue and differentiation into multiple cell lines. The three most commonly used stem cells are embryonic, induced pluripotent, and adult stem cells. Cells are combined with various natural and synthetic materials to form bioinks, which are used to fabricate scaffold-based or scaffold-free constructs. Computer aided design technology is combined with various bioprinting modalities including droplet-, extrusion-, or laser-based bioprinting to create tissue constructs. Each bioink and modality has its own advantages and disadvantages. Various materials and techniques are combined to maximize the benefits. Researchers have been successful in bioprinting cartilage, bone, cardiac, nervous, liver, and vascular tissues. However, a major limitation to clinical translation is building large-scale vascularized constructs. Many challenges must be overcome before this technology is used routinely in a clinical setting.

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“…In addition, they have shown that the encapsulated stem cells can be differentiated into osteoblasts and chondrocytes, further developing the tissue as a tracheal cartilage ring. Similarly, Nguyen et al printed human iPSCs into cartilage using a nanofibrillated cellulose (NFC) composite bioink together with irradiated human chondrocytes. These bio‐printed tissues are paving the path for novel treatments to repair damaged cartilage.…”

Section: Bio‐printing Stem Cellsmentioning

confidence: 99%

Engineering Concepts in Stem Cell Research

Narayanan

Mishra

Singh

et al. 2017

Biotechnology Journal

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The field of regenerative medicine integrates advancements made in stem cells, molecular biology, engineering, and clinical methodologies. Stem cells serve as a fundamental ingredient for therapeutic application in regenerative medicine. Apart from stem cells, engineering concepts have equally contributed to the success of stem cell based applications in improving human health. The purpose of various engineering methodologies is to develop regenerative and preventive medicine to combat various diseases and deformities. Explosion of stem cell discoveries and their implementation in clinical setting warrants new engineering concepts and new biomaterials. Biomaterials, microfluidics, and nanotechnology are the major engineering concepts used for the implementation of stem cells in regenerative medicine. Many of these engineering technologies target the specific niche of the cell for better functional capability. Controlling the niche is the key for various developmental activities leading to organogenesis and tissue homeostasis. Biomimetic understanding not only helped to improve the design of the matrices or scaffolds by incorporating suitable biological and physical components, but also ultimately aided adoption of designs that helped these materials/devices have better function. Adoption of engineering concepts in stem cell research improved overall achievement, however, several important issues such as long-term effects with respect to systems biology needs to be addressed. Here, in this review the authors will highlight some interesting breakthroughs in stem cell biology that use engineering methodologies.

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Cartilage Tissue Engineering by the 3D Bioprinting of iPS Cells in a Nanocellulose/Alginate Bioink

Cited by 378 publications

References 35 publications

Ambient‐Dried, 3D‐Printable and Electrically Conducting Cellulose Nanofiber Aerogels by Inclusion of Functional Polymers

Ambient‐Dried, 3D‐Printable and Electrically Conducting Cellulose Nanofiber Aerogels by Inclusion of Functional Polymers

Concise Review: Bioprinting of Stem Cells for Transplantable Tissue Fabrication

Engineering Concepts in Stem Cell Research

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