Cartilage tissue engineering of nasal septal chondrocyte-macroaggregates in human demineralized bone matrix

Liese, Juliane; Marzahn, Ulrike; Sayed, Karym El; Haisch, Andreas; Stoelzel, Katharina

doi:10.1007/s10561-012-9322-4

Cited by 15 publications

(9 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Resorbable scaffold materials could be chosen to hold cells, but the concern of the total mechanical stability of the cell‐scaffold construct should be taken care over time in vivo. Until now, various natural and synthetic materials have been tested with varying outcome (Chia et al, ; Glowacki & Mizuno, ; Liese et al, ; O'Brien, Harley, Yannas, & Gibson, ; Tuli, Li, & Tuan, ). The reason is mainly due to poor mechanical performance as well as local inflammatory reactions.…”

Section: Introductionmentioning

confidence: 99%

Novel decellularized animal conchal cartilage graft for application in human patient

Bhattacharya

Das

Joardar

et al. 2018

J Tissue Eng Regen Med

View full text Add to dashboard Cite

Restoration of the external ear and nose in human patients, in either congenital deformity or acquired defects, is a challenge in reconstructive surgery. Optimization of the currently available materials is necessary for rhinoplasty and microtia correction to avoid intraoperative manoeuvring and early rejection. The aim of this study was to develop cross-linked decellularized caprine conchal cartilages as biocompatible, robust, and non-toxic matrix template. The characterization of the decellularized tissue encompasses in vitro lymphoproliferation assay, cytotoxicity test, agar gel precipitation test, in vivo immunocompatibility study, histology, and determination of pro-inflammatory cytokines in animal model. Decellularized cartilage was implanted in human volunteer at R. G. Kar Medical College and Hospital, Kolkata, India, and samples were assessed histologically by retrieving those after 4 months. The processed cartilages were implanted in rhinoplasty (nine) and microtia patients (six) keeping autogenous cartilage graft as control up to 18 months after surgery. Primary outcomes were viability and safety of the material, both in animal model and human pre-application in actual site. Secondary outcomes included self-assessed clinical findings on gross examination. This study is under the ethical approval no. RKC/14 dated January 27, 2012. The in vitro cellular reactivity was less in processed cartilage protein than control. Histology of retrieved tissues in animal model and human volunteer showed no adverse reactions. Production of IL-2, IL-6, and TNF-α cytokines was lower at 4 weeks. The rhinoplasty and microtia operation in clinical patients utilizing the processed cartilage showed satisfactory recovery with improved facial look. These low cost, easily available, biocompatible, safe xenocartilage biomatrices of caprine conchal cartilage origin are very flexible in shape and size, enabling them as potential bioimplant for repair of nasal and auricular structure without any rejection or diverse biomedical applications.

show abstract

Section: Introductionmentioning

confidence: 99%

Novel decellularized animal conchal cartilage graft for application in human patient

Bhattacharya

Das

Joardar

et al. 2018

J Tissue Eng Regen Med

View full text Add to dashboard Cite

show abstract

“…In vitro ‐generated cartilage tissue is based on cells, either differentiated or stem cells, and various biomaterials. Collagen‐based resorbable matrices (Stone et al ., ; O'Brien et al ., ; Glowacki and Mizuno, ), natural and synthetic polymers (Glowacki and Mizuno, ; Langer and Vacanti, ; Vacanti et al ., ; Liese et al ., ) or hydrogels such as alginate (Liese et al ., ; Chia et al ., ) have been used frequently in this area. Although many more natural as well as synthetic materials have been tested, none has so far been proven to function optimally in cartilage tissue engineering of the head and neck area (Kusuhara et al ., ; Mafi et al ., ; Cao et al ., ).…”

Section: Introductionmentioning

confidence: 99%

Processed xenogenic cartilage as innovative biomatrix for cartilage tissue engineering: effects on chondrocyte differentiation and function

Schwarz¹,

Elsaesser²,

Koerber

et al. 2012

J Tissue Eng Regen Med

View full text Add to dashboard Cite

One key point in the development of new bioimplant matrices for the reconstruction and replacement of cartilage defects is to provide an adequate microenvironment to ensure chondrocyte migration and de novo synthesis of cartilage-specific extracellular matrix (ECM). A recently developed decellularization and sterilization process maintains the three-dimensional (3D) collagen structure of native septal cartilage while increasing matrix porosity, which is considered to be crucial for cartilage tissue engineering. Human primary nasal septal chondrocytes were amplified in monolayer culture and 3D-cultured on processed porcine nasal septal cartilage scaffolds. The influence of chondrogenic growth factors on neosynthesis of ECM proteins was examined at the protein and gene expression levels. Seeding experiments demonstrated that processed xenogenic cartilage matrices provide excellent environmental properties for human nasal septal chondrocytes with respect to cell adhesion, migration into the matrix and neosynthesis of cartilage-specific ECM proteins, such as collagen type II and aggrecan. Matrix biomechanical stability indicated that the constructs retrieve full stability and function during 3D culture for up to 42 days, proportional to collagen type II and GAG production. Thus, processed xenogenic cartilage offers a suitable environment for human nasal chondrocytes and has promising potential for cartilage tissue engineering in the head and neck region.

show abstract

“…The same experimentation performed with trabecular DBM failed. Moreover, it has been recently found that in experimental settings it is even possible to induce a good activity of chondrocytes if these cells are mixed with DBM, which can offer a good biomechanical stability [13]. DBM has been experimented for the treatment of many kinds of cysts and bony defects, with encouraging results, remarking its osteoinductive properties [14, 15].…”

Section: Resultsmentioning

confidence: 99%

Subchondral Bone Regenerative Effect of Two Different Biomaterials in the Same Patient

Cavallo

Buda

Vannini

et al. 2013

Case Reports in Orthopedics

View full text Add to dashboard Cite

This case report aims at highlighting the different effects on subchondral bone regeneration of two different biomaterials in the same patient, in addition to bone marrow derived cell transplantation (BMDCT) in ankle. A 15-year-old boy underwent a first BMDCT on a hyaluronate membrane to treat a deep osteochondral lesion (8 mm). The procedure failed: subchondral bone was still present at MRI. Two years after the first operation, the same procedure was performed on a collagen membrane with DBM filling the defect. After one year, AOFAS score was 100 points, and MRI showed a complete filling of the defect. The T2 mapping MRI after one year showed chondral tissue with values in the range of hyaline cartilage. In this case, DBM and the collagen membrane were demonstrated to be good biomaterials to restore subchondral bone: this is a critical step towards the regeneration of a healthy hyaline cartilage.

show abstract

Cartilage tissue engineering of nasal septal chondrocyte-macroaggregates in human demineralized bone matrix

Cited by 15 publications

References 35 publications

Novel decellularized animal conchal cartilage graft for application in human patient

Novel decellularized animal conchal cartilage graft for application in human patient

Processed xenogenic cartilage as innovative biomatrix for cartilage tissue engineering: effects on chondrocyte differentiation and function

Subchondral Bone Regenerative Effect of Two Different Biomaterials in the Same Patient

Contact Info

Product

Resources

About