Cartilage tissue engineering using human auricular chondrocytes embedded in different hydrogel materials

Yamaoka, Hisayo; Asato, Hirotaka; Ogasawara, Toru; Nishizawa, Satoru; Takahashi, Tatsuji; Nakatsuka, Takashi; Koshima, Isao; Nakamura, Kozo; Kawaguchi, Hiroshi; Chung, Ung‐il; Takato, Tsuyoshi; Hoshi, Kazuto

doi:10.1002/jbm.a.30655

Cited by 187 publications

(144 citation statements)

References 36 publications

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“…The chosen scaffold should provide the cells with the space to function and should support their activities. Every scaffold has specific properties that fit some kind of cells or mimic other tissues in mechanical strength, so we should regard all the characteristics of the target cells and tissues to choose the optimal scaffold (Yamaoka et al 2006). Also, proliferative activity is an important feature of cell samples that intended for therapeutic protocols.…”

Section: Discussionmentioning

confidence: 99%

The effects of synthetic and natural scaffolds on viability and proliferation of adipose-derived stem cells

Ghiasi

Kalhor

Qomi

et al. 2015

Frontiers in Life Science

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This study presents a comparative assessment of adipose-derived stem cell (ADSCs) proliferation rates and their viability on five different scaffolds. Five different biomaterial scaffolds were prepared: alginate, poly lactic-co-glycolic acid, fibrin glue, inactive platelet-rich plasma, and active plateletrich plasma (APRP). Stem cells were isolated from human adipose tissue. Flow cytometry analysis was performed. Specifically, adipogenesis/osteogenesis/chondrogenesis-associated genes expression was analyzed by real-time polymerase chain reaction. These cells were seeded in the prepared scaffolds. After 14 days, the proliferation and viability of MSCs were evaluated using an MTT assay. Also, stemness genes expression was analyzed with the reverse transcriptasepolymerase chain reaction (RT-PCR) method. In addition, the DNA content assay was also performed. The obtained results showed a significant difference between cell proliferation and viability of different scaffolds. APRP and alginate were shown to be the most and least suitable scaffolds in terms of enhancing cell proliferation and maintaining cell viability respectively (p < .05). RT-PCR reactions demonstrated the expression of the various stemness-related markers (Nanog, Octamer4A, and Sox2) when ADSC cells were grown separately on the five different scaffolds. Our study indicates that compared with the scaffolds, APRP could be the best scaffold for support of ADSC proliferation. ARTICLE HISTORY

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Section: Discussionmentioning

confidence: 99%

The effects of synthetic and natural scaffolds on viability and proliferation of adipose-derived stem cells

Ghiasi

Kalhor

Qomi

et al. 2015

Frontiers in Life Science

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“…The third hydrogel, Puramatrix® (3DM Medical Technology Inc., Cambridge, MA, USA), is a peptide composed of 16 amino acid residues (Zhang et al, 1993). When exposed to physiological salt conditions the peptide forms a hydrogel, which can support ECM production (Yamaoka et al, 2006). To prepare the Puramatrix® hydrogel a stock (1%) Puramatrix® was diluted in 20% (v/v) sucrose solution to a concentration of 0.5%.…”

Section: Hydrogel Culturementioning

confidence: 99%

The composition of hydrogels for cartilage tissue engineering can influence glycosaminoglycan profile

Wang

Hughes²,

Cartmell³

et al. 2010

eCM

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The injectable and hydrophilic nature of hydrogels makes them suitable candidates for cartilage tissue engineering. To date, a wide range of hydrogels have been proposed for articular cartilage regeneration but few studies have quantitatively compared chondrocyte behaviour and extracellular matrix (ECM) synthesis within the hydrogels. Herein we have examined the nature of ECM synthesis by chondrocytes seeded into four hydrogels formed by either temperature change, self-assembly or chemical crosslinking. Bovine articular cartilage chondrocytes were cultured for 14 days in Extracel®, Pluronic F127 blended with Type II collagen, Puramatrix® and Matrixhyal®. The discriminatory and sensitive technique of fluorophoreassisted carbohydrate electrophoresis (FACE) was used to determine the fine detail of the glycosaminoglycans (GAG); hyaluronan and chondroitin sulphate. FACE analysis for chondroitin sulphate and hyaluronan profiles in Puramatrix® closely matched that of native cartilage. For each hydrogel, DNA content, viability and morphology were assessed. Total collagen and total sulphated GAG production were measured and normalised to DNA content. Significant differences were found in total collagen synthesis. By day 14, Extracel® and Puramatrix® had significantly more total collagen than Matrixhyal® (1.77±0.26 μg and 1.97±0.26 μg vs. 0.60±0.26 μg; p<0.05). sGAG synthesis occurred in all hydrogels but a significantly higher amount of sGAG was retained within Extracel® at days 7 and 14 (p<0.05). In summary, we have shown that the biochemical and biophysical characteristics of each hydrogel directly or indirectly influenced ECM formation. A detailed understanding of the ECM in the development of engineered constructs is an important step in monitoring the success of cartilage regeneration strategies.

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“…Synthetic analogs of the natural ECMs were developed as 3-D scaffolds in an effort to accomplish in vivo-like environments in culture dishes, ex vivo tissue growth and engineering, and other scientific applications without posing health risks. One such material, PuraMatrix ™ , is a synthetic, self-assembling, peptide-based material that forms fibrous scaffolds and can be used for 3-D cell embedding or surface plating [19][20][21][22][23]. This nonanimal-derived material is non-immunogenic and can be used for in vivo studies.…”

Section: Introductionmentioning

confidence: 99%

Effects of extracellular matrix analogues on primary human fibroblast behavior

2008

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In vitro cell culture is a vital research tool for cell biology, pharmacology, toxicology, protein production, systems biology and drug discovery. Traditional culturing methods on plastic surfaces do not accurately represent the in vivo environment, and a paradigm shift from two-dimensional to three-dimensional (3-D) experimental techniques is underway. To enable this change, a variety of natural, synthetic and semi-synthetic extracellular matrix (ECM) equivalents have been developed to provide an appropriate cellular microenvironment. We describe herein an investigation of the properties of four commercially available ECM equivalents on the growth and proliferation of primary human tracheal scar fibroblast behavior, both in 3-D and pseudo-3-D conditions. We also compare subcutaneous tissue growth of 3-D encapsulated fibroblasts in vivo in two of these materials, Matrigel ™ and Extracel ™ . The latter shows increased cell proliferation and remodeling of the ECM equivalent. The results provide researchers with a rational basis for selection of a given ECM equivalent based on its biological performance in vitro and in vivo, as well as the practicality of the experimental protocols. Biomaterials that use a customizable glycosaminoglycan-based hydrogel appear to offer the most convenient and flexible system for conducting in vitro research that accurately translates to in vivo physiology needed for tissue engineering.

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Cartilage tissue engineering using human auricular chondrocytes embedded in different hydrogel materials

Cited by 187 publications

References 36 publications

The effects of synthetic and natural scaffolds on viability and proliferation of adipose-derived stem cells

The effects of synthetic and natural scaffolds on viability and proliferation of adipose-derived stem cells

The composition of hydrogels for cartilage tissue engineering can influence glycosaminoglycan profile

Effects of extracellular matrix analogues on primary human fibroblast behavior

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