Cartilage tissue engineering with silk scaffolds and human articular chondrocytes

Wang, Yongzhong; Blasioli, Dominick J.; Kim, Hyeon-Joo; Kim, Hyun Suk; Kaplan, David L.

doi:10.1016/j.biomaterials.2006.03.050

Cited by 390 publications

(232 citation statements)

References 43 publications

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“…Rapid degradation [170] [171]; [172]; [173]; [174] Alginate Aids re-differentiation of de-differentiated chondrocytes [66] In vivo injectable options [175] Abundant and low cost Certain cross-linkage can result in poor biocompatibility [184]; [185]; [186]; [187]; [188]; [189]; [182]; [190] Dermatan sulphate, [194]; [195] …”

Section: Conclusion and Future Perspectivesmentioning

confidence: 99%

Bioengineering of Articular Cartilage: Past, Present and Future

Felimban

Moulton

et al. 2013

Regen. Med.

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The treatment of cartilage defects poses a clinical challenge owing to the lack of intrinsic regenerative capacity of cartilage. The use of tissue engineering techniques to bioengineer articular cartilage is promising and may hold the key to the successful regeneration of cartilage tissue. Natural and synthetic biomaterials have been used to recreate the microarchitecture of articular cartilage through multilayered biomimetic scaffolds. Acellular scaffolds preserve the microarchitecture of articular cartilage through a process of decellularization of biological tissue. Although promising, this technique often results in poor biomechanical strength of the graft. However, biomechanical strength could be improved if biomaterials could be incorporated back into the decellularized tissue to overcome this limitation.

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Section: Conclusion and Future Perspectivesmentioning

confidence: 99%

Bioengineering of Articular Cartilage: Past, Present and Future

Felimban

Moulton

et al. 2013

Regen. Med.

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“…In all tested scaffolds, transcript levels of cartilage-related genes, like Col 2, Agr, Sox 9, were upregulated. [27][28][29][30][31][32] Two different types of hydrogels were examined in the present study: alginate and alginate/agarose. A 14-day incubation period was selected to allow for sufficient redifferentiation.…”

Section: Human Chondrocyte Gene Expression In Vitromentioning

confidence: 99%

Quantitative analysis of gene expression in human articular chondrocytes assigned for autologous implantation

Barlič

Drobnič

Maličev

et al. 2008

Journal Orthopaedic Research

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Autologous chondrocyte implantation (ACI) relies on the implantation of in vitro expanded cells. The aim was to study the dedifferentiation of human articular chondrocytes under different cultivating conditions [days 0-10 in the primary culture (P0); passages in a monolayer from P0 to P3; monolayer vs. alginate and monolayer vs. alginate/agarose hydrogels] using real-time PCR analysis. The relative gene expressions for collagen type I and II, aggrecan and versican were quantified and the corresponding differentiation indexes (Col2/Col1, Agr/Ver) were calculated. The values of both differentiation indexes decreased exponentially with time in the P0 monolayer culture, and continued with a significant decrease over the subsequent monolayer passages. On the contrary, the chondrocytes seeded in either of the hydrogels significantly increased the indexes compared to their parallel monolayer cultures. These results indicate that alginate and alginate/agarose hydrogels offer an appropriate environment for human articular chondrocytes to redifferentiate after being expanded in vitro. Therefore the three-dimensional (3D) hydrogel chondrocyte cultures present not only surgical, but also biological advantage over the classic suspension-periosteum chondrocyte implantation. ß

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“…These differentiated MSC's can then be seeded into a 3-D scaffold made from natural or man-made materials such as collagen, fibrin hydrogels, PLGA/PLLA copolymers 4 , chitosan 5 , silk 6 and others.…”

Section: Introductionmentioning

confidence: 99%

A review of some recent developments in polarization-sensitive optical imaging techniques for the study of articular cartilage

Matcher

2009

Journal of Applied Physics

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This article reviews recent developments in the optical imaging of articular cartilage using polarized-light methods, with an emphasis on tools that could be of use in tissue engineering approaches to treatment. Both second harmonic generation microscopy and polarizationsensitive optical coherence tomography are described and their potential role in the treatment of cartilage disorders such as osteoarthritis is suggested. Key results are reviewed and future developments discussed.2

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Cartilage tissue engineering with silk scaffolds and human articular chondrocytes

Cited by 390 publications

References 43 publications

Bioengineering of Articular Cartilage: Past, Present and Future

Bioengineering of Articular Cartilage: Past, Present and Future

Quantitative analysis of gene expression in human articular chondrocytes assigned for autologous implantation

A review of some recent developments in polarization-sensitive optical imaging techniques for the study of articular cartilage

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