Cartridge based nucleic acid amplification test: a sensitive diagnostic tool for tuberculosis on fine needle aspirates samples

Patil, Suwarna; Dhage, Shweta M.; Umap, Pradeep; Ghorpade, Shivahari V.; Patharwat, Shyamkant

doi:10.18203/2394-6040.ijcmph20201465

Cited by 4 publications

(3 citation statements)

References 8 publications

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“…Our study included 100 clinically suspected cases of cervical tuberculous lymphadenitis. Maximum number of patients (70%) were in age group of 11-30 years, which was similar to study conducted by Patil SB et al [7] where majority of patients (53%) were in this age group, also in Tadesse. M et al [8] maximum no of patients (58%) were in 16-30 years age group and Swati Nair et al [9] reported 59% cases in the age group of 11-30 years.…”

Section: Discussionsupporting

confidence: 88%

“…Our study showed female predominance which was similar to Patil SB et al [7], Tadesse M et al [8] and Gautam et al [10], but in contrast to Swati Nair et al [9] which reported male to female ratio 1.27:1. In our study most of patients(70%) did not have constitutional symptoms which is similar to Jha et al (12) and contrast to Dandapat et al [13].…”

Section: Discussionsupporting

confidence: 87%

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Cervical tuberculous lymphadenitis

Pradhan¹,

Trilochan

Roy³

et al. 2022

ijhs

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Background: Diagnosis of cervical tuberculous lymphadenitis has been challenging to clinicians as it mimics multiple diseases. USG has upper hand over other diagnostic modalities as it is non- invasive, low cost, time saving and guided procedures like FNAC and core needle biopsy can be performed. Methods: This is a prospective observational study conducted in 100 patients from June 2019 to May 2020 in Dept of Pulmonary medicine, PRM Medical College and Hospital, Baripada. A complete clinical examination, routine blood investigations, CXR, USG Neck, FNAC and CBNAAT were done and data was recorded. Results: Maximum numbers of patients were in age group of 11-20 years (38%) followed by 21-30 years (32%) and presented between the duration of 1-3 months. There were 44(44%) males and 56(56%) females; male to female ratio was 1:1.3. Most of the patients (70%) did not have any constitutional symptoms and maximum number of patients i.e. 53% had matting of LN followed by discrete node 29% whereas 10% patients presented with abscess and 8% had discharging sinuses. In USG, 46 (46%) showed cervical lymphadenopathy with tubercular aetiology with sensitivity and specificity of 90% and 50% respectively with accuracy of 88%.

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Section: Discussionsupporting

confidence: 88%

Section: Discussionsupporting

confidence: 87%

Cervical tuberculous lymphadenitis

Pradhan¹,

Trilochan

Roy³

et al. 2022

ijhs

View full text Add to dashboard Cite

show abstract

“…5 Patil et al noted the sensitivity of CBNAAT to be similar to the present study, but specificity was much higher in the reference study (80.57%) as compared to the present study (50%). 25 Sensitivity, specificity, positive predictive value, and negative predictive value of CBNAAT were documented to be 92.7%, 98.9%, 97.1%, and 97.2% respectively in a study by Kumar et al 26…”

Section: Discussionmentioning

confidence: 95%

Extra pulmonary tuberculosis manifestation in head and neck and its diagnosis with recent tools like cartridge based nucleic acid amplification test

Vatsyayan

Sachdeva

2021

Int J Otorhinolaryngol Head Neck Surg

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Background: The study aimed to assess the clinical manifestations of extra-pulmonary tuberculosis (head and neck) in diagnosed pulmonary tuberculosis (TB) patients and without pulmonary TB and to determine the sensitivity and specificity of cartridge based nucleic acid amplification test (CBNAAT) in the diagnosis of extra-pulmonary TB.Methods: The study was conducted as an observational study at the Department of Otorhinolaryngology, tertiary care center, Jabalpur (Madhya Pradesh) for a period of 18 months i.e. from 01 March 2019 to 31 August 2020 on 30 patients presenting with lesions in ear, nose and throat (ENT), head and neck region. All necessary investigations with TB workup were done. Sputum samples were analyzed by CBNAAT on Xpert- Mycobacterium tuberculosis complex/resistance to rifampin (MTB/RIF). Patients were treated accordingly. Appropriate surgical intervention was done in necessary cases. Using statistical analysis, the sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV), and diagnostic accuracy was calculated for CBNAAT. Results: Neck swelling and ear discharge were the most common features observed in 66.7% of cases. In cases with pulmonary TB, systemic features were the most predominant features whereas in cases with extra-pulmonary TB, local features were predominant. Sensitivity, specificity, PPV and NPV of CBNAAT were documented to be 85%, 50%, 89.5% and 40% respectively.Conclusions: TB of the head and neck are commonly encountered in clinical practice. The patients usually present with variable clinical manifestations depending upon the site of involvement. The middle ear is the most common site affected by head and neck tuberculosis. CBNAAT is a sensitive tool for the diagnosis of extra-pulmonary TB with and without associated pulmonary TB.

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Combined modalities for the rapid diagnosis of patients with suspected tuberculous lymphadenitis: A cross-sectional study

Sayed,

Sane

2024

Lung India

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Background: In settings with high burden of extrapulmonary tuberculosis, the use of various diagnostic modalities can result in superior and quick diagnosis leading to prompt initiation of treatment. Objective: This study assessed the diagnostic performance of the fine-needle aspiration cytology (FNAC), Ziehl–Neelsen (ZN) stain, fluorescence microscopy (FM) and cartridge-based nucleic acid amplification test (CBNAAT) in patients with suspected tuberculous lymphadenitis (TBLN). Methods: This cross-sectional studyodes, who underwent FNAC. The FNAC samples were subjected to cytomorphological examinati involved 255 patients clinically suspected of TBLN with palpable lymph non, ZN stain, FM and CBNAAT. The diagnostic performance of each modality was compared with CBNAAT, and combined performance was determined. The diagnostic performance of CBNAAT was determined by comparing it with composite reference standard. Results: Of 255 patients, 148 (58.04%) showed features of TBLN on FNAC. The presence of epithelioid cell granulomas with caseous necrosis (67.57%) was the predominant cytomorphological pattern. On ZN stain, FM and CBNAAT, 31 (20.95%), 63 (42.57%) and 100 (67.57%) patients were found to be positive for TBLN, respectively. The sensitivity, specificity, positive predictive value and negative predictive value of CBNAAT was 58.59%, 91.83%, 92% and 58.06%, respectively. Finally, the combination of ZN stain, FM and CBNAAT led to the detection of 88.51% patients with TBLN. Conclusion: Combined use of ZN stain, FM and CBNAAT leads to superior and swift diagnosis of patients with clinically suspected TBLN.

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Cartridge based nucleic acid amplification test: a sensitive diagnostic tool for tuberculosis on fine needle aspirates samples

Cited by 4 publications

References 8 publications

Cervical tuberculous lymphadenitis

Cervical tuberculous lymphadenitis

Extra pulmonary tuberculosis manifestation in head and neck and its diagnosis with recent tools like cartridge based nucleic acid amplification test

Combined modalities for the rapid diagnosis of patients with suspected tuberculous lymphadenitis: A cross-sectional study

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