Carvacrol oil inhibits biofilm formation and exopolysaccharide production of Enterobacter cloacae

Liu, Fang; Panpan, Jin; Sun, Zhilan; Du, Lei; Wang, Daoying; Zhao, Tong; Doyle, Michael P.

doi:10.1016/j.foodcont.2020.107473

Cited by 40 publications

(24 citation statements)

References 54 publications

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“…Besides, the high accelerating voltages used during SEM measurements can have also negatively affected the structural appearance of the biofilm structure [ 73 ]. However, similar accelerating voltages, as used in the present study, were previously reported for biofilm research [ 44 , 71 ].…”

Section: Resultsmentioning

confidence: 72%

“…This highlights that the structural analysis of biofilms is essential in addition to culture-based analysis, as the latter does not consider the biofilm morphology and structure and might lead to false predictions about the biofilm-forming ability if used as a standalone method. Besides morphological observation, EPS production can be quantified with techniques such as the phenol–sulfuric acid method [ 71 ].…”

Section: Resultsmentioning

confidence: 99%

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Biofilm-Forming Ability of Microbacterium lacticum and Staphylococcus capitis Considering Physicochemical and Topographical Surface Properties

Zand

Pfanner

Domig

et al. 2021

Foods

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Biofilm characteristics of Microbacterium lacticum D84 (M. lacticum) and Staphylococcus capitis subsp. capitis (S. capitis) on polytetrafluoroethylene and AISI-304 stainless steel at early- (24, 48 h) and late-stage (144, 192 h) biofilm formation were investigated. M. lacticum biofilm structure was more developed compared to S. capitis, representing vastly mature biofilms with a strongly developed amorphous matrix, possibly extracellular polymeric substances (EPSs), at late-stage biofilm formation. S. capitis showed faster growth behavior but still resulted in a relatively flat biofilm structure. Strong correlations were found between several roughness parameters and S. capitis surface coverage (r ≥ 0.98), and between total surface free energy (γs) and S. capitis surface coverage (r = 0.89), while M. lacticum remained mostly unaffected. The pronounced ubiquitous biofilm characteristics make M. lacticum D84 a suitable model for biofilm research. Studying biofilm formation of these bacteria may help one understand bacterial adhesion on interfaces and hence reduce biofilm formation in the food industry.

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Section: Resultsmentioning

confidence: 72%

Section: Resultsmentioning

confidence: 99%

Biofilm-Forming Ability of Microbacterium lacticum and Staphylococcus capitis Considering Physicochemical and Topographical Surface Properties

Zand

Pfanner

Domig

et al. 2021

Foods

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“…Prior to the assay, a loopful of S. aureus cells were streaked on brain–heart infusion (BHI) agar (Qingdao Hope Bio-Technology Co., Ltd.) and incubated at 37 ℃ for 24 h. One single colony was transferred into 5 mL of BHI broth (Qingdao Hope Bio-Technology Co., Ltd.). S. aureus cells were cultured to exponential phase under shaking at 200 rpm (37 °C) for 24 h. The concentration of S. aureus cells in the exponential phase reached approximately 9 1 g CFU/mL [21] , [22] .…”

Section: Methodsmentioning

confidence: 99%

The combination of ultrasound and chlorogenic acid to inactivate Staphylococcus aureus under planktonic, biofilm, and food systems

Sun

Wang

Sun

et al. 2021

Ultrasonics Sonochemistry

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Highlights The synergistic bactericidal effect had produced by ultrasound (US) and chlorogenic acid (CA). US plus CA changed the morphology of S. aureus cells.. US plus CA led the leakage of internal constituents. US plus CA reduced the concentration of polysaccharide in biofilms. US plus CA reduced S. aureus in mutton by 1.14 log CFU/g.

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“…This phenomenon causes changes in EPS structural components and weakens the irreversible adhesion of bacteria, which is easy to fall of the surface, thereby reducing the early stage biofilms formation. A study about Enterobacter cloacae showed that thymol could also inhibit the curli fimbriae csgABCEFG to affect initial adhesion in addition to affecting exopolysaccharide synthesis genes ( Liu et al, 2020a ).…”

Section: Inhibiting Eps Productionmentioning

confidence: 99%

Strategies for Interfering With Bacterial Early Stage Biofilms

Zhang

Lin

et al. 2021

Front. Microbiol.

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Biofilm-related bacteria show high resistance to antimicrobial treatments, posing a remarkable challenge to human health. Given bacterial dormancy and high expression of efflux pumps, persistent infections caused by mature biofilms are not easy to treat, thereby driving researchers toward the discovery of many anti-biofilm molecules that can intervene in early stage biofilms formation to inhibit further development and maturity. Compared with mature biofilms, early stage biofilms have fragile structures, vigorous metabolisms, and early attached bacteria are higher susceptibility to antimicrobials. Thus, removing biofilms at the early stage has evident advantages. Many reviews on anti-biofilm compounds that prevent biofilms formation have already been done, but most of them are based on compound classifications to introduce anti-biofilm effects. This review discusses the inhibitory effects of anti-biofilm compounds on early stage biofilms formation from the perspective of the mechanisms of action, including hindering reversible adhesion, reducing extracellular polymeric substances production, interfering in the quorum sensing, and modifying cyclic di-GMP. This information can be exploited further to help researchers in designing new molecules with anti-biofilm activity.

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Carvacrol oil inhibits biofilm formation and exopolysaccharide production of Enterobacter cloacae

Cited by 40 publications

References 54 publications

Biofilm-Forming Ability of Microbacterium lacticum and Staphylococcus capitis Considering Physicochemical and Topographical Surface Properties

Biofilm-Forming Ability of Microbacterium lacticum and Staphylococcus capitis Considering Physicochemical and Topographical Surface Properties

The combination of ultrasound and chlorogenic acid to inactivate Staphylococcus aureus under planktonic, biofilm, and food systems

Strategies for Interfering With Bacterial Early Stage Biofilms

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