Cas-based bacterial detection: recent advances and perspectives

Lan, Huatao; Shu, Weitong; Jiang, Dan; Yu, Luxin; Xu, Guangxian

doi:10.1039/d3an02120c

Analyst

2024

DOI: 10.1039/d3an02120c

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Cas-based bacterial detection: recent advances and perspectives

Huatao Lan,

Weitong Shu,

Dan Jiang

et al.

Abstract: Rapid and sensitive detection of pathogens. CRISPR/Cas has strong potential and application prospects in the field of bacterial detection.

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2024

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Cited by 2 publications

References 147 publications

(152 reference statements)

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Advances in Rapid Detection Methods for African Swine Fever Virus

Gu,

Yin,

Huang

et al. 2024

Chinese Journal of Analytical Chemistry

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Advances in Rapid Detection Methods for African Swine Fever Virus

Gu,

Yin,

Huang

et al. 2024

Chinese Journal of Analytical Chemistry

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Nitrogen-Doped Carbon Dots: A New Powerful Fluorescent Dye with Substantial Effect on Bacterial Cell Labeling

Lee,

Zheng,

Chen

et al. 2024

ACS Omega

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Carbon dots (CDs)minute carbon nanoparticles with remarkable luminescent properties, photostability, and low toxicityshow potential for various applications. CDs synthesized using citric acid and urea are the least toxic to biological environments. Here, we aimed to explore the effect of CDs synthesized using citric acid and urea at 50, 33, and 25% (CDs 1/1, 1/2, and 1/3, respectively) weight ratios in a microwave on bacterial cell fluorescence sensing and labeling. The nanoscale properties of CDs were investigated via transmission electron microscopy and dynamic light scattering particle size analysis. X-ray powder diffraction confirmed the graphitic structures of CDs. X-ray photoelectron spectroscopy revealed that the nitrogen content increased gradually with increasing urea ratios, indicating functional group changes. Transient photoluminescence decay periods demonstrated superior fluorescence intensity of CDs 1/3 under blue, green, and red lights. The use of CDs was notably more efficient than traditional methods in staining bacterial cells. Fluorescence microscopy of 10 g-positive and 10 g-negative bacteria revealed enhanced staining of Gram-positive strains, with CDs 1/3 presenting the best results. The CDs exhibited excellent photostability, maintaining poststaining fluorescence for 100 min, surpassing the performance of conventional dyes. CDs could serve as potential fluorescent dyes for the rapid discrimination of Gram-positive and Gram-negative bacteria.

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Cas-based bacterial detection: recent advances and perspectives

Abstract: Rapid and sensitive detection of pathogens. CRISPR/Cas has strong potential and application prospects in the field of bacterial detection.

Cited by 2 publications

References 147 publications

Advances in Rapid Detection Methods for African Swine Fever Virus

Advances in Rapid Detection Methods for African Swine Fever Virus

Nitrogen-Doped Carbon Dots: A New Powerful Fluorescent Dye with Substantial Effect on Bacterial Cell Labeling

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