Cas9/gRNA-Mediated Mutations in PtrFLA40 and PtrFLA45 Reveal Redundant Roles in Modulating Wood Cell Size and SCW Synthesis in Poplar

Zhen, Cheng; Hua, Xinguo; Jiang, Xue; Tong, Guimin; Li, Chunming; Yang, Chuanping; Cheng, Yuxiang

doi:10.3390/ijms24010427

Cited by 7 publications

(1 citation statement)

References 55 publications

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“…Subsequent studies have shown that AtFLA16 deletion leads to reduced stem length and changes in biomechanical properties, suggesting that it plays a role in the synthesis and function of plant secondary cell walls. What's more, the genome-wide of the FLA gene family has been identi ed in many other plant species, such as rice (Oryza sativa), wheat (Triticum aestivum) [15], textile hemp (Cannabis sativa) [18], eucalypt (Eucalyptus grandis) [19], Nicotiana benthamiana [14], cotton (Gossypium hirsutum) [20], pear (Pyrus bretschneideri) [21], banana (Musa) [22], Chinese cabbage (Brassica rapa) [23], poplar (Populus trichocarpa) [24]. Some preliminary evidence suggests that FLA may be important in plant growth and development, such as regeneration of cell wall [9], development of ber [25], communication of cell-to-cell, and in response to cold stress [9,22] and pathogen [17].…”

Section: Introductionmentioning

confidence: 99%

Identification of fasciclin-like arabinogalactan protein family genes associated with fruit ripening in tomato

Wang

Tuerdiyusufu

et al. 2023

Preprint

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Background: Tomato (Solanum lycopersicum) is a model plant for fruit ripening, of which contains a series of complex physiological and biochemical process. Fasciclin-like arabinogalactan proteins (FLAs) are a subclass of arabinogalactan proteins (AGPs) involved in cell wall formation and intracellular signal transduction. However, its functional mechanism in plant development including fruit ripening is rarely studied. In the present study, we identified four species tomatoes’ FLAs and analyzed their structural characteristics, evolutionary history and expression patterns during fruit development to mine candidate genes and determine their potential role in tomato fruit ripening. Results: In the present study, 18 ,21, 22 and 22 FLAs were identified from the S. lycopersicum, S. pimpinellifolium, S. pennellii, and S. lycopersicoides, respectively. These proteins were divided into four groups by evolutionary and structural characteristics, and each group of FLAs in FAS structure domain, glycosylphosphatidylinositol (GPI) and the number there are similarities. The FLAs in four species are relatively conserved, and most of them are distributed at both ends of chromosomes. The FLA family members are amplified and evolved mainly by means of segmental duplication and purifying selection. Similar transcripts and expression patterns analysis among them revealed their regulatory roles in tomato fruit ripening. More intresting, in the WGCNA module constructed by the combination of tomato fruit transcriptome and targeted carotenoid metabolome, several SlFLAs co-expressed with genes enriched in secondary metabolism. Conclusion: The FLAs gene family found in four species tomatoes and provides valid information in their little-studied studies on the regulation of fruit ripening. Combined with the detection of a key metabolite of tomato fruit ripening, carotenoids, which broadens the idea of biological functional analysis of SlFLAs and provides a theoretical basis and candidate genes for improving tomato fruit quality.

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Section: Introductionmentioning

confidence: 99%

Identification of fasciclin-like arabinogalactan protein family genes associated with fruit ripening in tomato

Wang

Tuerdiyusufu

et al. 2023

Preprint

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Genome Editing in Forest Trees

Bruegmann,

Fendel,

Zahn

et al. 2023

A Roadmap for Plant Genome Editing

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Since the first CRISPR/Cas-mediated genome editing of poplar in 2015, an increasing number of tree species are being genome-edited. Although the availability of genome sequences, tissue culture and transformation systems are limiting factors, research is ongoing on advanced methods such as DNA-free genome editing and gene targeting approaches in addition to the optimisation of single gene knockouts. These can be used to address ambitious issues and perform genome editing more accurately, which has implications for the legal assessment of edited trees. Once technically established, CRISPR/Cas can be used to circumvent specific challenges related to forest tree species, e.g., longevity and extended vegetative phases, and to modify traits relevant for breeding, whether for direct application or to elucidate the genetic basis of individual traits. Not least due to climate change, adaptation to abiotic stress such as drought stress as well as biotic stresses caused by pathogens are strongly in focus. For the use as a renewable resource and as a carbon sink, wood productivity in forest trees as well as wood properties are of interest. In biosafety assessments, tree-specific aspects have to be considered, which result, among other aspects, from the long lifespan.

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Analysis of genes related to xylem cell wall development based on transcriptomics in Populus alba ‘Berolinensis’ tension wood

Feng,

He,

et al. 2024

J. For. Res.

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Populus alba ‘Berolinensis’ is a fast-growing, high-yielding species with strong biotic and abiotic stress resistance, and widely planted for timber, shelter belts and aesthetic purposes. In this study, molecular development is explored and the important genes regulating xylem formation in P. alba ‘Berolinensis’ under artificial bending treatments was identified. Anatomical investigation indicated that tension wood (TW) was characterized by eccentric growth of xylem and was enriched in cellulose; the degree of lignification was lower than for normal wood (NW) and opposite wood (OW). RNA-Seq-based transcriptome analysis was performed using developing xylem from three wood types (TW, OW and NW). A large number of differentially expressed genes (DEGs) were screened and 4889 counted. In GO and KEGG enrichment results, genes involved in plant hormone signal transduction, phenylpropanoid biosynthesis, and cell wall and secondary cell wall biogenesis play major roles in xylem development under artificial bending. Eight expansin (PalEXP) genes were identified from the RNA-seq data; four were differentially expressed during tension wood formation. Phylogenetic analysis indicated that PalEXLB1 belongs to the EXPB subfamily and that the other PalEXPs are members of the EXPA subfamily. A transcriptional regulatory network construction showed 10 transcription factors located in the first and second layers upstream of EXP, including WRKY, ERF and bHLH. RT‒qPCR analysis in leaves, stems and roots combined with transcriptome analysis suggests that PalEXPA2, PalEXPA4 and PalEXPA15 play significant regulatory roles in cell wall formation during tension wood development. The candidate genes involved in xylem cell wall development during tension wood formation marks an important step toward identifying the molecular regulatory mechanism of xylem development and wood property improvement in P. alba ‘Berolinensis’.

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Cas9/gRNA-Mediated Mutations in PtrFLA40 and PtrFLA45 Reveal Redundant Roles in Modulating Wood Cell Size and SCW Synthesis in Poplar

Cited by 7 publications

References 55 publications

Identification of fasciclin-like arabinogalactan protein family genes associated with fruit ripening in tomato

Identification of fasciclin-like arabinogalactan protein family genes associated with fruit ripening in tomato

Genome Editing in Forest Trees

Analysis of genes related to xylem cell wall development based on transcriptomics in Populus alba ‘Berolinensis’ tension wood

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