2023
DOI: 10.1101/2023.09.05.556296
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Cas9 is mostly orthogonal to human systems of DNA break sensing and repair

Ekaterina A. Maltseva,
Inna A. Vasil’eva,
Nina A. Moor
et al.

Abstract: CRISPR/Cas9 system is а powerful gene editing tool based on the RNA-guided cleavage of target DNA. The Cas9 activity can be modulated by proteins involved in DNA damage signalling and repair due to their interaction with double- and single-strand breaks (DSB and SSB, respectively) generated by wild-type Cas9 or Cas9 nickases. Here we address the interplay between Streptococcus pyogenes Cas9 and key DNA repair factors, including poly(ADP-ribose) polymerase 1 (SSB/DSB sensor), its closest homolog poly(ADP-ribose… Show more

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