CASA Assessment of Kinematic Parameters of Ram Spermatozoa and their Relationship to Migration Efficiency in Ruminant Cervical Mucus

Robayo, I; Montenegro, V. A.; Valdés, Cecilia T.; Cox, JF

doi:10.1111/j.1439-0531.2007.00920.x

Cited by 66 publications

(56 citation statements)

References 32 publications

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“…The inconsistency between the CASA parameter values obtained in this study with those reported in the studies of Robayo et al (2008), Munyai (2012), and Palacín et al (2013) emphasizes the importance of standardizing CASA assessment protocols to allow for comparison of studies from different laboratories. Standardization would enable researchers to potentially correlate CASA results to the in vivo scenario of sperm transport in the female reproductive tract.…”

Section: Resultscontrasting

confidence: 82%

A novel flush technique to simulate natural dispersal of spermatozoa in the female reproductive tract and expedite motility assessment of fresh ejaculated Merino (<i>Ovis aries</i>) sperm

Boshoff¹,

Lambrechts²,

Maree³

et al. 2018

SA J. An. Sci.

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Motility is an important criterion in the assessment and quantification of the quality of ejaculated and epididymal sperm samples. Ovine sperm spermatozoa are particularly susceptible to cryodamage, and shortening the interval from collection to cryostorage may potentially minimize the negative effects of cryopreservation, thereby improving the post-thaw viability of ram spermatozoa. The use of the swim-up technique (SUT) in quantifying spermatozoa motility is well documented, especially for the isolation of highly motile spermatozoa for assisted reproductive purposes. However, this technique is time consuming and involves a swim-up period of 10 minutes before the motility of a sample is recorded by using computerassisted sperm analysis (CASA) software such as the Sperm Class Analyser® (SCA®). The novel flush technique (FT) allows for capturing of sperm motility tracks via the SCA® system shortly after semen collection, that is, within a minute. This study compared fresh ejaculated sperm motility traits by using the SUT and FT. Motility evaluations were performed using 45 semen samples obtained from 15 adult Merino rams (Ovis aries) at weekly intervals. Motility recordings were captured at 100 frames per second, using a calibrated 20 µm deep Leja slide. The percentage total motile spermatozoa of samples subjected to the FT was 89.2%, which was significantly higher than that recorded by the SUT (83.9%). The results also indicated that the wobble (WOB) parameter showed significantly higher values when using the FT, and parameters curvilinear velocity (VCL) and amplitude of the lateral head displacement (ALH) indicated significantly higher values when using the SUT. Establishing the ideal spermatozoa concentration and analysis of sperm subpopulation motility characteristics would assist in the optimization of the FT, and its use in CASA motility analysis of ovine spermatozoa. Standardization of CASA analysis of ovine semen samples, which would enable the selection of quality spermatozoa samples for use in field insemination (fresh samples) or in vitro fertilization programs, and laparoscopic AI cryopreserved samples warrants further investigation.

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Section: Resultscontrasting

confidence: 82%

A novel flush technique to simulate natural dispersal of spermatozoa in the female reproductive tract and expedite motility assessment of fresh ejaculated Merino (<i>Ovis aries</i>) sperm

Boshoff¹,

Lambrechts²,

Maree³

et al. 2018

SA J. An. Sci.

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“…The lack of a significant correlation between the ability of epididymal spermatozoa to penetrate cervical mucus and its kinematic motility parameters suggests that in the current study, the mucus-penetrating ability of spermatozoa was not influenced by its motility or velocity but rather by some unknown means conferred by exposure to seminal plasma. This contradicts published reports in the ram (Suttiyotin et al 1992, Robayo et al 2008) and human (Keel & Webster 1988), who linked increased mucus penetration with increased velocity and motility. However, in the latter study, 10-15% of patients with otherwise normal semen parameters demonstrated poor mucus-penetrating ability, suggesting that mucus penetration tests provide information about sperm function not attainable by motility analysis alone (Keel & Webster 1988).…”

Section: Cervicalcontrasting

confidence: 55%

Seminal plasma aids the survival and cervical transit of epididymal ram spermatozoa

Rickard¹,

Pini²,

Soleilhavoup³

et al. 2014

Reproduction

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Seminal plasma purportedly plays a critical role in reproduction, but epididymal spermatozoa are capable of fertilisation following deposition in the uterus, calling into question the biological requirement of this substance. Through a combination of direct observation of spermatozoa in utero using probe-based Confocal Laser Endomicroscopy, in vivo assessment of sperm fertility and in vitro analysis of various sperm functional parameters, this study investigated the role of seminal plasma in spermatozoa transit through the cervix of the ewe. Following deposition in the cervical os, epididymal spermatozoa previously exposed to seminal plasma displayed an enhanced ability to traverse the cervix as evidenced by both significantly higher pregnancy rates and numbers of spermatozoa observed at the utero-tubal junction when compared with epididymal spermatozoa not previously exposed to seminal plasma. The beneficial effect of seminal plasma on sperm transport was clearly localised to transit through the cervix as pregnancy rates of spermatozoa deposited directly into the uterus were unaffected by exposure to seminal plasma. This phenomenon was not explained by changes to sperm motion characteristics, as seminal plasma had no effect on the motility, kinematic parameters or mitochondrial membrane potential of spermatozoa. Rather, in vitro testing revealed that seminal plasma improved the ability of epididymal spermatozoa to penetrate cervical mucus recovered from ewes in oestrus. These results demonstrate that the survival and transport of ram spermatozoa through the cervix of the ewe is not linked to their motility or velocity but rather the presence of some cervical penetration trait conferred by exposure to seminal plasma.

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“…Los iones de bicarbonato activan la cAMP, lo que ha llevado a la protein Kinase C a ser uno de los reguladores del movimiento espermático (Mortimer, 1997), y cambios en la composición o el pH del plasma seminal pueden ser la causa de las diferencias en motilidad individual. Robayo et al, (2008) indican que VCL y VAP se correlacionan de manera positiva con la habilidad de migrar en el moco cervical de la oveja, y VAP con la eficiencia de ésta migración, pero encontraron un efecto negativo entre el desplazamiento lateral de la cabeza y la migración.…”

Section: Los Estudios Existentes De Efectos Del H Rosa-sinensis Son unclassified

Calidad seminal en ovinos pelibuey con inclusión de Hibiscus rosa-sinensis en la dieta

Gamboa¹,

Sierra²,

Loría³

et al. 2015

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: 07-11-2014: 07-11- Aceptación: 11-07-2015 Abstract Introduction: feeding for sheep production in the tropics accounts from 48 to 90% of total production cost; additionally, the use of grains also makes producers dependent on the international market. Moderate weight gains in rams could be obtained with foliage from tropical trees; however, some plants such as Hibiscus Rosa-sinensis have compounds that could act as phytoestrogens, which could compromise its potential use as animal feed.Method: twelve Pelibuey rams, aged 24±3 months old, 45±6 kg live weight and a body condition score of 2.3±0.3 in a scale from 1 to 5, were assigned to one of two treatments for 45 days. T1 (n = 12), commercial feed (500 g d -1 by sheep) with 16% CP, and T2 (n = 12), 20% of H.rosasinensis (451 g DM kg MV ~ 1.6 kg d-1 per animal). Both treatments were supplemented with (Pennisetum purpureum x P. Typhoides var. grass. Parameters of semen quality were evaluated: ejaculate volume (Vol, mL) and concentration (EC, millions per mL) and eight individual motility parameters were used: curvilinear speed (VCL, µ/s), straight line velocity Calidad seminal en ovinos pelibuey con inclusión de Hibiscus rosa-sinensis en la dieta

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CASA Assessment of Kinematic Parameters of Ram Spermatozoa and their Relationship to Migration Efficiency in Ruminant Cervical Mucus

Cited by 66 publications

References 32 publications

A novel flush technique to simulate natural dispersal of spermatozoa in the female reproductive tract and expedite motility assessment of fresh ejaculated Merino (<i>Ovis aries</i>) sperm

A novel flush technique to simulate natural dispersal of spermatozoa in the female reproductive tract and expedite motility assessment of fresh ejaculated Merino (<i>Ovis aries</i>) sperm

Seminal plasma aids the survival and cervical transit of epididymal ram spermatozoa

Calidad seminal en ovinos pelibuey con inclusión de Hibiscus rosa-sinensis en la dieta

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