Cascade DNA nanomachine and exponential amplification biosensing

Xu, Jianguo; Wu, Zai‐Sheng; Shen, Weiyu; Xu, Huo; Li, Hongling; Lee, Jia

doi:10.1016/j.bios.2015.05.045

Cited by 41 publications

(14 citation statements)

References 21 publications

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“…Molecular Probes : By using The dual‐cyclical nucleic acid strand displacement polymerization amplification strategy to the generation of DNA nanomachine, the biosensing signal was exponentially amplified, enabling the quantification of p53 gene in the wide concentration range from 0.05 to 150 × 10 −9 m with the detection limit of 50 × 10 −12 m and easily distinguishing the mutant gene from the wild‐type …”

Section: Biomedical Applicationsmentioning

confidence: 99%

Programmable and Multifunctional DNA‐Based Materials for Biomedical Applications

et al. 2018

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DNA encodes the genetic information; recently, it has also become a key player in material science. Given the specific Watson-Crick base-pairing interactions between only four types of nucleotides, well-designed DNA self-assembly can be programmable and predictable. Stem-loops, sticky ends, Holliday junctions, DNA tiles, and lattices are typical motifs for forming DNA-based structures. The oligonucleotides experience thermal annealing in a near-neutral buffer containing a divalent cation (usually Mg ) to produce a variety of DNA nanostructures. These structures not only show beautiful landscape, but can also be endowed with multifaceted functionalities. This Review begins with the fundamental characterization and evolutionary trajectory of DNA-based artificial structures, but concentrates on their biomedical applications. The coverage spans from controlled drug delivery to high therapeutic profile and accurate diagnosis. A variety of DNA-based materials, including aptamers, hydrogels, origamis, and tetrahedrons, are widely utilized in different biomedical fields. In addition, to achieve better performance and functionality, material hybridization is widely witnessed, and DNA nanostructure modification is also discussed. Although there are impressive advances and high expectations, the development of DNA-based structures/technologies is still hindered by several commonly recognized challenges, such as nuclease instability, lack of pharmacokinetics data, and relatively high synthesis cost.

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Section: Biomedical Applicationsmentioning

confidence: 99%

Programmable and Multifunctional DNA‐Based Materials for Biomedical Applications

et al. 2018

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“…To solve this problem, researchers have developed other DNA cascade nanomachines to amplify the signal while using fuel strands as catalyst to initiate the reaction. In 2015, Jia and co‐workers developed a cascade DNA nanomachine to detect tumor related genes in vitro. They adopted a dual‐cyclical nucleic acid strand‐displacement polymerization (dual‐CNDP) strategy to amplify the signal.…”

Section: Strand‐mediated Displacement Motorsmentioning

confidence: 99%

“…These smart carriers are aptly titled nanomachines and are capable of conformational and positional action in response to environmental stimuli, allowing them to be driven to specific regions or functionalized to only deliver the cargo where it is most needed . The nanomachines can also be adapted to function as modalities for detection and amplification, for the diagnostics of hard‐to‐detect diseases and the better understanding of nanomachine transportation on the nanoscale …”

Section: Introductionmentioning

confidence: 99%

Bioderived DNA Nanomachines for Potential Uses in Biosensing, Diagnostics, and Therapeutic Applications

Angell

Kai

Xie

et al. 2018

Adv Healthcare Materials

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Beside its genomic properties, DNA is also recognized as a novel material in the field of nanoengineering. The specific bonding of base pairs can be used to direct the assembly of highly structured materials with specific nanoscale features such as periodic 2D arrays, 3D nanostructures, assembly of nanomaterials, and DNA nanomachines. In recent years, a variety of DNA nanomachines are developed because of their many potential applications in biosensing, diagnostics, and therapeutic applications. In this review, the fuel-powered motors and secondary structure motors, whose working mechanisms are inspired or derived from natural phenomena and nanomachines, are discussed. The combination of DNA motors with other platforms is then discussed. In each section of these motors, their mechanisms and their usage in the biomedical field are described. Finally, it is believed that these DNA-based nanomachines and hybrid motifs will become an integral point-of-care diagnostics and smart, site-specific therapeutic delivery.

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“…In recent years, there are many reports about nucleic acid detection for its huge potential in clinical disease diagnosis (Xuan and Hsing, 2014;Cai et al, 2014;Ghindilis et al, 2015;Hun et al, 2015;Xu et al, 2015;Zhu et al, 2015b). Lots of ultrasensitive nucleic acid testing methods have been proposed.…”

Section: Introductionmentioning

confidence: 99%

An electrochemical biosensor for double-stranded Wnt7B gene detection based on enzymatic isothermal amplification

Chen

et al. 2016

Biosensors and Bioelectronics

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Cascade DNA nanomachine and exponential amplification biosensing

Cited by 41 publications

References 21 publications

Programmable and Multifunctional DNA‐Based Materials for Biomedical Applications

Programmable and Multifunctional DNA‐Based Materials for Biomedical Applications

Bioderived DNA Nanomachines for Potential Uses in Biosensing, Diagnostics, and Therapeutic Applications

An electrochemical biosensor for double-stranded Wnt7B gene detection based on enzymatic isothermal amplification

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