Cascade ultrafiltration systems—Integrated processes for purification and concentration of lysozyme

Mayani, Mukesh; Filipe, Carlos D. M.; Ghosh, Raja

doi:10.1016/j.memsci.2009.10.016

Cited by 41 publications

(17 citation statements)

References 21 publications

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“…This method has been reported to successfully achieve protein fractionation through UF with several advantages compared with the conventional separation using biology/ chemistry methods [4][5][6] . The main advantage being generating a product with high purity level and low toxicity 7 .…”

Section: Introductionmentioning

confidence: 99%

The performance of bovine serum albumin filtration by using polyethersulfone-Tetronic 304 blend Ultrafiltration Membrane

2019

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Background: Membrane technology has been widely applied for protein purification. In applications for protein separation, a membrane with stable filtration performance is necessary. In this work, two types of hollow fiber membranes with different characteristic were used to study the filtration profile of bovine serum albumin. Methods: A single piece of hollow fiber module was used for ultrafiltration testing using UF0 and UFT304 membranes. Flux and rejection of BSA solution were collected based on a pressure-driven inside filtration model. Results: Ultrafiltration experiments showed that the flux of UFT304 membrane was higher than that of UF0 membrane in all applied pressure condition. Solute rejection was achieved up to 90% for ultrafiltration of BSA solution by using UF0 membrane obtained on the operating pressure of 0.5 atm. Conclusion: In general, UFT304 membranes has better ultrafiltration performance for BSA separation than UF0 membranes. The UFT304 membrane has a more stable flux for up to two hours of filtration.

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Section: Introductionmentioning

confidence: 99%

The performance of bovine serum albumin filtration by using polyethersulfone-Tetronic 304 blend Ultrafiltration Membrane

2019

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“…Nowadays a number of membrane cascade configurations have been studied. Mayani et al used various cascaded ultrafiltration systems for continuous fractionation of plasma proteins, for purification of plant monoclonal antibody and for purification and concentration of lysozyme . In another cascade study Mohanty and Ghosh continuously fractionated humanized monoclonal antibodies by using countercurrent ultrafiltration cascades.…”

Section: Introductionmentioning

confidence: 99%

“…14 15 for purification of plant monoclonal antibody 16 and for purification and concentration of lysozyme. 17 In another cascade study Mohanty and Ghosh 18 continuously fractionated humanized monoclonal antibodies by using countercurrent ultrafiltration cascades. Caus et al 19 studied counter-current nanofiltration cascades with up to four stages for the separation of xylose and maltose.…”

Section: Introductionmentioning

confidence: 99%

Continuous purification of galacto-oligosaccharide mixtures by using cascaded membrane filtration

Patil

Schotel

Gómez

et al. 2015

J. Chem. Technol. Biotechnol.

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“…Ultrafiltration and nanofiltration are the most frequently used membrane technologies for biotechnological and pharmaceutical purposes. Ultrafiltration is widely used for the separation and fractionation of proteins and other biological molecules and nanofiltration cascades have been implemented for pesticide removal, solvent exchange, solvent recovery, and separation and fractionation of pharmaceutical solutes …”

Section: Introductionmentioning

confidence: 99%

Analysis and optimization of continuous organic solvent nanofiltration by membrane cascade for pharmaceutical separation

2014

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The major part of the production costs of pharmaceuticals can be imputed to the downstream processing, where membrane technologies have to deal with some challenges as separations involving solutes with similar sizes or solvent recovery and recycling. This work contributes to the progress in the design of continuous organic solvent nanofiltration systems for this purpose and includes the configuration of dual membrane cascades, sensitivity analysis of the operation variables, and economic optimization as innovations. Analyzed configurations include multistage cascades up to three stages, and dual membrane cascades up to five stages. The total costs (TC) were chosen as the formulated objective function to minimize in the economic optimization strategy. The treatment of the residual stream leaving the system resulted the main cost of the process (more than 85% for dual cascades), but the solvent recovery units can significantly reduce the TC (64-77% depending on the required solvent quality).

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Cascade ultrafiltration systems—Integrated processes for purification and concentration of lysozyme

Cited by 41 publications

References 21 publications

The performance of bovine serum albumin filtration by using polyethersulfone-Tetronic 304 blend Ultrafiltration Membrane

The performance of bovine serum albumin filtration by using polyethersulfone-Tetronic 304 blend Ultrafiltration Membrane

Continuous purification of galacto-oligosaccharide mixtures by using cascaded membrane filtration

Analysis and optimization of continuous organic solvent nanofiltration by membrane cascade for pharmaceutical separation

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