Cascaded valorization of seaweed using microbial cell factories

Poblete‐Castro, Ignacio; Hoffmann, Sarah Lisa; Becker, Jörg; Wittmann, Christoph

doi:10.1016/j.copbio.2020.02.008

Cited by 36 publications

(15 citation statements)

References 91 publications

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“…Green seaweeds are emerging feedstocks for sulfated polysaccharide-based biopolymer products and are expected to gain many industrial interests in the near future. Even though there are challenges in the production of seaweed-based products, the preparation of biomaterials and chemicals from green seaweeds is still growing and the bioprocessing technologies for the utilization of green seaweeds are developing [ 94 ]. To make these technologies economically feasible, it is important to develop simple extraction routes with high yield and purity, improve the availability of seaweeds for operation, and develop downstream process to recover value-added materials.…”

Section: Discussionmentioning

confidence: 99%

A Short Review on the Valorization of Green Seaweeds and Ulvan: FEEDSTOCK for Chemicals and Biomaterials

et al. 2020

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This short review analyzed the recent trend towards, progresses towards the preparation of chemicals of, and value-added biomaterials from marine macroalgae resources, especially green seaweeds and their derived ulvan polysaccharides for various applications. In recent years, ulvan both in pristine and modified forms has gained a large amount of attention for its effective utilization in various areas due to its unique physiochemical properties, lack of exploration, and higher green seaweed production. The pristine form of ulvan (sulfated polysaccharides) is used as a bio-component; food ingredient; or a raw material for the production of numerous chemicals such as fuels, cosmetics, and pharmaceuticals, whereas its modified form is used in the sector of composites, membranes, and scaffolds, among others, because of its physicochemical properties. This review highlights the utilization of green seaweed and its derived ulvan polysaccharides for the preparation of numerous chemicals (e.g., solvents, fuel, and gas) and also value-added biomaterials with various morphologies (e.g., gels, fibers, films, scaffolds, nanomaterials, and composites).

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Section: Discussionmentioning

confidence: 99%

A Short Review on the Valorization of Green Seaweeds and Ulvan: FEEDSTOCK for Chemicals and Biomaterials

et al. 2020

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“…An example of this includes ACE-1 inhibitors such as bioactive peptides from species of red, green, and brown seaweeds [ 112 , 114 , 219 ]. The human consumption of seaweeds has been a part of Asian cultures for decades [ 220 ] both directly and in the form of functional food ingredients [ 221 ]. It has been linked to a decrease in the incidence of coronary heart disease in Japan [ 222 ].…”

Section: Introductionmentioning

confidence: 99%

Macroalgal Proteins: A Review

Brien

Hayes

Sheldrake

et al. 2022

Foods

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Population growth is the driving change in the search for new, alternative sources of protein. Macroalgae (otherwise known as seaweeds) do not compete with other food sources for space and resources as they can be sustainably cultivated without the need for arable land. Macroalgae are significantly rich in protein and amino acid content compared to other plant-derived proteins. Herein, physical and chemical protein extraction methods as well as novel techniques including enzyme hydrolysis, microwave-assisted extraction and ultrasound sonication are discussed as strategies for protein extraction with this resource. The generation of high-value, economically important ingredients such as bioactive peptides is explored as well as the application of macroalgal proteins in human foods and animal feed. These bioactive peptides that have been shown to inhibit enzymes such as renin, angiotensin-I-converting enzyme (ACE-1), cyclooxygenases (COX), α-amylase and α-glucosidase associated with hypertensive, diabetic, and inflammation-related activities are explored. This paper discusses the significant uses of seaweeds, which range from utilising their anthelmintic and anti-methane properties in feed additives, to food techno-functional ingredients in the formulation of human foods such as ice creams, to utilising their health beneficial ingredients to reduce high blood pressure and prevent inflammation. This information was collated following a review of 206 publications on the use of seaweeds as foods and feeds and processing methods to extract seaweed proteins.

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“…In addition, it can produce killer toxins that inhibit the growth of competing microorganisms ( 12 ). Microbial production in cell factories with such properties has great potential in applications such as bio-production of third-generation biofuels ( 13–15 ). Despite the obvious potential of D. hansenii , the field of basic research in this organism is underdeveloped, and heterologous production possibilities of this yeast remain unexploited due to the lack of robust genetic engineering techniques.…”

Section: Introductionmentioning

confidence: 99%

A CRISPR/Cas9 method facilitates efficient oligo-mediated gene editing in Debaryomyces hansenii

et al. 2021

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The halophilic and osmotolerant yeast Debaryomyces hansenii has a high potential for cell factory applications due to its resistance to harsh environmental factors and compatibility with a wide substrate range. However, currently available genetic techniques does not allow the full potential of D. hansenii as a cell factory to be harnessed. Moreover, most of the currently available tools rely on the use of auxotrophic markers that are not suitable in wild-type prototrophic strains. In addition, the preferred non-homologous end-joining (NHEJ) DNA damage repair mechanism pose further challenges when precise gene targeting is required. In this study, we present a novel plasmid based CRISPRCUG/Cas9 method for easy and efficient gene editing of the prototrophic strains of D. hansenii. Our toolset design is based on a dominant marker and facilitates quick assembly of the vectors expressing Cas9 and single or multiple sgRNAs that provides possibility for multiplex gene engineering even in prototrophic strains. Moreover, we have constructed an NHEJ deficient D. hansenii that enable our CRISPRCUG/Cas9 tools to support highly efficient introduction of point mutations and single/double gene deletions. Importantly, we also demonstrate that 90-nt single stranded DNA oligonucleotides are sufficient to direct repair of DNA breaks induced by sgRNA-Cas9 resulting in precise edits reaching 100% efficiencies. In conclusion, tools developed in this study will greatly advance basic and applied research in D. hansenii. In addition, we envision that our tools can be rapidly adapted for gene editing of other non-conventional yeast species including the ones belonging to the CUG clade.

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Cascaded valorization of seaweed using microbial cell factories

Cited by 36 publications

References 91 publications

A Short Review on the Valorization of Green Seaweeds and Ulvan: FEEDSTOCK for Chemicals and Biomaterials

A Short Review on the Valorization of Green Seaweeds and Ulvan: FEEDSTOCK for Chemicals and Biomaterials

Macroalgal Proteins: A Review

A CRISPR/Cas9 method facilitates efficient oligo-mediated gene editing in Debaryomyces hansenii

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