Case–Control Study of Human Papillomavirus and Oropharyngeal Cancer

Kreimer, Aimée R.; Viscidi, Raphael P.; Pawlita, Michael; Fakhry, Carole; Koch, Wayne M.; Westra, William H.; Gillison, Maura L.

doi:10.1056/nejmoa065497

Cited by 2,376 publications

(2,114 citation statements)

References 36 publications

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“…Part of the reasons behind this phenomenon is the increased incidence of high risk human papillomavirus (HPV) related OPSCC [1][2][3]. Interestingly, multiple retrospective studies have shown that patients with HPVpositive OPSCC have a better prognosis compared to patients with HPV-negative tumors [4,5].…”

Section: Introductionmentioning

confidence: 99%

Mutation and Transcriptional Profiling of Formalin-Fixed Paraffin Embedded Specimens as Companion Methods to Immunohistochemistry for Determining Therapeutic Targets in Oropharyngeal Squamous Cell Carcinoma (OPSCC): A Pilot of Proof of Principle

Saba

Wilson

Doho

et al. 2014

Head and Neck Pathol

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The role of molecular methods in the diagnosis of head and neck cancer is rapidly evolving and holds great potential for improving outcomes for all patients who suffer from this diverse group of malignancies. However, there is considerable debate as to the best clinical approaches, particularly for Next Generation Sequencing (NGS). The choices of NGS methods such as whole exome, whole genome, whole transcriptomes (RNA-Seq) or multiple gene resequencing panels, each have strengths and weakness based on data quality, the size of the data, the turnaround time for data analysis, and clinical actionability. There have also been a variety of gene expression signatures established from microarray studies that correlate with relapse and response to treatment, but none of these methods have been implemented as standard of care for oropharyngeal squamous cell carcinoma (OPSCC). Because many genomic methodologies are still far from the capabilities of most clinical laboratories, we chose to explore the use of a combination of off the shelf targeted mutation analysis and gene expression analysis methods to complement standard anatomical pathology methods. Specifically, we have used the Ion Torrent AmpliSeq cancer panel in combination with the NanoString nCounter Human Cancer Reference Kit on 8 formalin-fixed paraffin embedded (FFPE) OPSCC tumor specimens, (4) HPVpositive and (4) HPV-negative. Differential expression analysis between HPV-positive and negative groups showed that expression of several genes was highly likely to correlate with HPV status. For example, WNT1, PDGFA and OGG1 were all over-expressed in the positive group. Our results show the utility of these methods with routine FFPE clinical specimens to identify potential therapeutic targets which could be readily applied in a clinical trial setting for clinical laboratories lacking the instrumentation or bioinformatics infrastructure to support comprehensive genomics workflows. To the best of our knowledge, these preliminary experiments are among the earliest to combine both mutational and gene expression profiles using Ion Torrent and NanoString technologies. This reports serves as a proof of principle methodology in OPSCC.Electronic supplementary material The online version of this article

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Section: Introductionmentioning

confidence: 99%

Mutation and Transcriptional Profiling of Formalin-Fixed Paraffin Embedded Specimens as Companion Methods to Immunohistochemistry for Determining Therapeutic Targets in Oropharyngeal Squamous Cell Carcinoma (OPSCC): A Pilot of Proof of Principle

Saba

Wilson

Doho

et al. 2014

Head and Neck Pathol

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“…Oral cancer significantly impacts quality of life, physically and psychosocially, and the genetic links between some types of oral-pharyngeal carcinomas and ano-genital malignancies have been established via the human papillomavirus (HPV), particularly the strains 16 and 18 as found by Herrero et al (2003) and others (Anhang, Goodman, & Goldie, 2004;Cox, 2000;D'Souza et al, 2007;Ha & Califano, 2004). Since HPV 16 is the most predominant strain in ano-genital carcinomas, its transmission to the oropharyngeal region can occur through the mouth, especially during oral sex in sexually active individuals (Scully, 2005).…”

Section: Introductionmentioning

confidence: 99%

Café Discussions on Oral Sex, Oral Cancer, and HPV Infection: Summative Report

Brondani

2010

Arch Sex Behav

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Recent emphasis has been placed on the potential links between oral sex, HPV infection, and oral cancer development. Such links were addressed by researchers, clinicians, and the community during two Café Scientifique discussions in October and November 2008, in Vancouver, Canada. The Cafes gathered panels of experts on oral pathology, dentistry, oncology, social work, and community-based research who interacted with an audience of policy makers, health care administrators, sociologists, sexologists, pharmacists, clinical and social researchers, social workers, technicians, and graduate, undergraduate, and high school students. This commentary summarizes the main points discussed during these two events to encourage a worldwide open dialogue about potential risks for oral cancer beyond tobacco smoking and excessive alcohol consumption as such malignancies have high mortality and morbidity, but are yet preventable diseases.

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“…Worldwide, two of the high-risk HPV types, HPV16 and 18, are responsible for approximately 70% of cervical cancer and are also the HPV types most often found in HPV-associated cancers at other sites. A lower percentage of several additional cancers can be associated with persisting high-risk HPV infection, including cancers of anogenital epithelium (vulval, vaginal, penile, anal), with HPV-attributable fractions between 40-90%, and some cancers of other mucosal epithelial surfaces [11]. Most HPV infections are asymptomatic and resolve spontaneously with a median time to resolution of less than 1 year [12].…”

Section: Human Papillomaviruses (Hpv)mentioning

confidence: 99%

Prevention of cancer through immunization: Prospects and challenges for the 21st century

2007

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Case–Control Study of Human Papillomavirus and Oropharyngeal Cancer

Abstract: Oral HPV infection is strongly associated with oropharyngeal cancer among subjects with or without the established risk factors of tobacco and alcohol use.

Cited by 2,376 publications

References 36 publications

Mutation and Transcriptional Profiling of Formalin-Fixed Paraffin Embedded Specimens as Companion Methods to Immunohistochemistry for Determining Therapeutic Targets in Oropharyngeal Squamous Cell Carcinoma (OPSCC): A Pilot of Proof of Principle

Mutation and Transcriptional Profiling of Formalin-Fixed Paraffin Embedded Specimens as Companion Methods to Immunohistochemistry for Determining Therapeutic Targets in Oropharyngeal Squamous Cell Carcinoma (OPSCC): A Pilot of Proof of Principle

Café Discussions on Oral Sex, Oral Cancer, and HPV Infection: Summative Report

Prevention of cancer through immunization: Prospects and challenges for the 21st century

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