2022
DOI: 10.5489/cuaj.7850
|View full text |Cite
|
Sign up to set email alerts
|

Case series – COVID-19 is unlikely to affect male fertility: Results of histopathological and reverse transcriptase polymerase chain reaction analysis

Abstract: Introduction: We aimed to analyze the testicular histopathology of men who died with active COVID-19 infection. Methods: We performed autopsy of eight consecutive men who died of COVID-19 pneumonia. Lung and testis tissue of all men were stained for SARS-CoV-2 nucleocapsid, ACE2 receptor immunohistochemistry (IHC). H&E was performed to assess for spermatogenesis and evidence of testicle tissue damage. Reverse transcriptase polymerase chain reaction (RTPCR) analysis for SARS-CoV-2 was performed on matched l… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
4
1

Citation Types

0
7
0

Year Published

2022
2022
2024
2024

Publication Types

Select...
4

Relationship

0
4

Authors

Journals

citations
Cited by 4 publications
(7 citation statements)
references
References 18 publications
0
7
0
Order By: Relevance
“…Since that study, conflicting evidence on the presence of SARS-CoV-2 in testicular histology has been published. Despite another study also confirming positive SARS-CoV-2 RT-PCR in the testis, three more recent studies reported a negative test in either all or the majority of their subjects ( 15 18 ). Masterson et al.…”
Section: Effect Of Sars-cov-2 On Reproductive Tissuesmentioning
confidence: 90%
See 3 more Smart Citations
“…Since that study, conflicting evidence on the presence of SARS-CoV-2 in testicular histology has been published. Despite another study also confirming positive SARS-CoV-2 RT-PCR in the testis, three more recent studies reported a negative test in either all or the majority of their subjects ( 15 18 ). Masterson et al.…”
Section: Effect Of Sars-cov-2 On Reproductive Tissuesmentioning
confidence: 90%
“…However, IHC in the context of SARS-CoV-2 positive participants remains ambiguous. This is because there is no positive control for IHC staining, studies that reported positive findings have not shown a significant correlation within positive SARS-CoV-2 RT-PCR participants, and the molecules targeted in IHC staining have not been consistent throughout all the previous studies ( 15 18 ). Despite these higher levels of ACE2, IHC staining has been correlated with worse clinical outcomes regarding testis injury severity ( 19 ).…”
Section: Effect Of Sars-cov-2 On Reproductive Tissuesmentioning
confidence: 96%
See 2 more Smart Citations
“…Nonetheless, the testes are the key organ for the male reproductive system, and expression of SARS CoV2 in the testes has been revealed by many researchers. However, the detection of SARS CoV2 antibodies in testicular tissue is tough and non-specific (Parra-Medina et al 2021;Masterson et al 2022). In recent times, single-cell RNA sequencing can help us to effectively outline the cell types.…”
Section: Presence Of Acein Testes-role In Leydig and Sertoli Cellsmentioning
confidence: 99%