2022
DOI: 10.1177/02611929221117999
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Case Studies Exemplifying the Transition to Animal Component-free Cell Culture

Abstract: Cell culture techniques are strongly connected with modern scientific laboratories and production facilities. Thus, choosing the most suitable medium for the cells involved is vital, not only directly to optimise cell viability but also indirectly to maximise the reliability of the experiments performed with the cells. Fetal bovine or calf serum (FBS or FCS, respectively) is the most commonly used cell culture medium supplement, providing various nutritional factors and macromolecules essential for cell growth… Show more

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Cited by 8 publications
(9 citation statements)
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“…87 In addition, living animal bodies are used as manufacturing devices, and animals can suffer from side effects and long-lasting consequences of these procedures (another example of this is growing cancer cells in mice to produce Matrigel ® ). 90 He highlighted a number of case studies exemplifying the transition to animal component-free cell culture, 91 and listed various replacements for animal-derived reagents — for example, the use of human platelet lysate (hPL) as a supplement for human mesenchymal stem/stromal cells, and the use of xeno-free chemically defined media. 91,92 Finally, Mr Weber provided guidelines to find, 93 as well as produce, optimised serum-free cell culture media, 94 and shared details of a practical workshop on replacing FBS.…”
Section: Session 4: ‘Disease In a Dish’ Modelsmentioning
confidence: 99%
See 1 more Smart Citation
“…87 In addition, living animal bodies are used as manufacturing devices, and animals can suffer from side effects and long-lasting consequences of these procedures (another example of this is growing cancer cells in mice to produce Matrigel ® ). 90 He highlighted a number of case studies exemplifying the transition to animal component-free cell culture, 91 and listed various replacements for animal-derived reagents — for example, the use of human platelet lysate (hPL) as a supplement for human mesenchymal stem/stromal cells, and the use of xeno-free chemically defined media. 91,92 Finally, Mr Weber provided guidelines to find, 93 as well as produce, optimised serum-free cell culture media, 94 and shared details of a practical workshop on replacing FBS.…”
Section: Session 4: ‘Disease In a Dish’ Modelsmentioning
confidence: 99%
“…90 He highlighted a number of case studies exemplifying the transition to animal component-free cell culture, 91 and listed various replacements for animal-derived reagents — for example, the use of human platelet lysate (hPL) as a supplement for human mesenchymal stem/stromal cells, and the use of xeno-free chemically defined media. 91,92 Finally, Mr Weber provided guidelines to find, 93 as well as produce, optimised serum-free cell culture media, 94 and shared details of a practical workshop on replacing FBS. 95 He briefly touched on the replacements available for other animal-derived reagents and endotoxin/pyrogen tests, 91 hence setting the path to achieving a truly animal-free laboratory working environment, to the benefit of animals, scientists and patients.…”
Section: Session 4: ‘Disease In a Dish’ Modelsmentioning
confidence: 99%
“…Furthermore, an immense step forward could be achieved if peer-reviewers would demand to verify experimental results in a xeno-free or chemically defined environment [47]. Such a step is in the very own interest of reviewers, applicants and journals as it can ensure reproducibility for published results.…”
Section: Increasing Awareness About Non-animal Products/ingredientsmentioning
confidence: 99%
“…Serum-free approaches are further explored in the article by Weber et al, 2 who demonstrate, by providing a range of examples, that the transition to animal component-free cell culture is possible and achievable. They highlight three different scenarios and provide case studies for each.…”
Section: Editorialmentioning
confidence: 99%