“…Morris [33] also concluded that attempts to improve the quality of frozen-thawed ejaculated or epididymal sperm by density gradient centrifugation before low-dose insemination did not benefit fertility. In another study using an aged stallion ejaculating low numbers of sperm, processing the entire ejaculate for each low-dose insemination by either centrifugation or density gradient centrifugation did not significantly improve pregnancy rates [37]. In contrast, few reports of improved pregnancy rates for some subfertile stallions exist when sperm separation techniques are first used to process the inseminate.…”