Case Study: Recombinant Bromelain Selection

Amid, Azura; Ismail, Nurul Azira; Arshad, Zatul Iffah Mohd

doi:10.1007/978-3-319-12397-4_10

Cited by 2 publications

(2 citation statements)

References 74 publications

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“…Bromelain bolsters the immune system by increasing cytokine production, which are hormones produced by white blood cells to improve immunity. Several studies have established the ability of bromelain to remove T‐cell CD44 molecules from lymphocytes (Amid, Ismail, & Arshad, ). Furthermore, total serum protein in fish is responsible for the innate immune response, and higher levels of this provide stronger responses (Sahu, Das, Mishra, Pradhan, & Sarangi, ).…”

Section: Discussionmentioning

confidence: 99%

The use of bromelain as a feed additive in fish diets: Growth performance, intestinal morphology, digestive enzyme and immune response of juvenile Sterlet (Acipenser ruthenus)

et al. 2019

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Bromelain is a proteolytic enzyme extracted from Ananas comosus and has great potential to affect several physiological functions. The current study examined the effect of bromelain added to commercial feed at concentrations of 0 g (control), 10 g (B1) and 20 g (B2) per kg diet on growth, feed utilization, intestinal morphology, digestive enzyme and immune response of juvenile Sterlet (Acipenser ruthenus) for 56 days. At the end of this experiment, the highest final body weight was detected in fish fed diet of B2 compared to control. The protein content of whole fish was higher in fish fed diet of B1 and B2, but the content of calcium, iron, copper and zinc was noted lower in fillets of fish fed diet of B1 and B2. Supplementation with bromelain significantly increased the height of mucosal folds, enterocytes and the supranuclear zone of the epithelial cells compared to control diet. The enzymatic activity of lipase and pepsin was significantly (p < 0.05) higher in fish fed diet of B1 and B2. The highest activity of lysozyme, total protein level and total immunoglobulin and the proliferative activity of T and B cells were detected in fish fed diet of B2 compared to control, where no significant (p > 0.05) difference was found in ceruloplasmin, metabolic activity of spleen macrophages and potential killing activity of spleen phagocytes between different treatments.

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Section: Discussionmentioning

confidence: 99%

The use of bromelain as a feed additive in fish diets: Growth performance, intestinal morphology, digestive enzyme and immune response of juvenile Sterlet (Acipenser ruthenus)

et al. 2019

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“…Bonggol nanas kaya akan bromelin. Bromelin meningkatkan sistem kekebalan tubuh dengan cara meningkatkan produksi sitokin, yaitu hormon yang diproduksi oleh sel darah putih untuk meningkatkan kekebalan (Amid et al, 2015). Buah nanas juga memiliki potensi sebagai sumber antioksidan, karena pada nanas mengandung banyak vitamin C, dan terdapat kandungan tanin dan flavonoid sebagai anti bakteri yang cukup tinggi (Lingga, 2012).…”

Section: Pengukuran Berat Mutlakunclassified

Addition of Pineapple Hump Extract to Feed and Probiotics in Cultivation Media on the Performance of Catfish (Clarias gariepinus

Kartina

Afiansyah

Awaludin

2023

Arwana: Scientific Journal of Water Aquaculture

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Catfish is a consumption fish that is in great demand so that production continues to be increased. Pineapple hump are known to be rich in bromelain enzymes. The bromelain enzyme has properties similar to proteolytic enzymes, which have the ability to hydrolyze proteins. This study aims to determine the optimal dose of pineapple hump extract in feed on the growth performance of the Sangkuriang catfish. The test fish used were catfish seeds measuring 3-5 cm and weighing ± 3 g. This study used an experimental method in a completely randomized design (CRD) consisting of 4 treatments and 3 replications, with P0 (control) 0% pineapple hump extract; P1 dose 0.75%; P2 1.5% and P3 3% extract/kg feed. Probiotics were added to the rearing medium to maintain water quality as much as 1.5 ml/liter. The results showed that the addition of pineapple hump extract in the P2 treatment with a dose of 1.5% showed the highest value at absolute length of 4.4 cm, at absolute weight of 2.3 g, Specific Growth Rate (SGR) of 4.5% and the survival rate is 86% and the lowest value for each observation is in the P0 (control) treatment. However, the statistical test did not show a significant difference in results. Based on the results of the study, pineapple hump extract can be used in feed to increase the growth of the Sangkuriang catfish at a dose of 1.5%/kg of feed.

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Case Study: Recombinant Bromelain Selection

Cited by 2 publications

References 74 publications

The use of bromelain as a feed additive in fish diets: Growth performance, intestinal morphology, digestive enzyme and immune response of juvenile Sterlet (Acipenser ruthenus)

The use of bromelain as a feed additive in fish diets: Growth performance, intestinal morphology, digestive enzyme and immune response of juvenile Sterlet (Acipenser ruthenus)

Addition of Pineapple Hump Extract to Feed and Probiotics in Cultivation Media on the Performance of Catfish (Clarias gariepinus

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