Casein calcium-binding peptides: Preparation, characterization, and promotion of calcium uptake in Caco-2 cell monolayers

Structural characterisation of deer sinew peptides as calcium carriers, their promotion of MC3T3-E1 cell proliferation and their effect on bone deposition in mice

“…33 Sheep collagen peptide–calcium chelates were found to be rich in Ala, Glu, Asp and Gly. 34 The relative percentage contents of Asp, Thr, Ser and Glu increased to different degrees after the casein calcium-binding peptide was chelated with calcium, 35 suggesting that these amino acids play an important role in the chelation process with calcium. At high pH, conditions corresponding to calcium uptake with high binding affinity include Asp, Glu, Leu, His, Ala, and Gly.…”

Study on the mechanism of targeted regulation of casein linear epitopes by three animal‐derived proteases under the optimal antigen inhibition rate conditions

“…As shown in Figure 3C, CN, PED, PAD and CHD exhibited the same characteristic peaks, and similar results were also reported by Lao et al . (2023). After hydrolysis, the amide I band of the three hydrolysates shifted from 1633.68 cm −1 (CN) to 1604.06 cm −1 (PED), 1612.75 cm −1 (PAD) and 1631.23 cm −1 (CHD), and the amide II band shifted from 1386.21 cm −1 (CN) to 1385.75 cm −1 (PED), 1385.83 cm −1 (PAD) and 1386.10 cm −1 (CHD).…”

Purification, Identification, Chelation Mechanism, and Calcium Absorption Activity of a Novel Calcium-Binding Peptide from Peanut (Arachis hypogaea) Protein Hydrolysate

“…The sequence and composition of amino acid and binding capacity with calcium at the side of the C/N terminal were important factors for selecting the peptide sequence. The amino acids Asp and Glu were considered to be the main site of peptide-calcium chelation and played a vital role in calcium chelation . The carboxylic acid groups on the side chain of the biopeptide derived from the acidic amino acids Asp and Glu, which affect the local charge density and provided a favorable environment for calcium binding .…”

“…The amino acids Asp and Glu were considered to be the main site of peptide-calcium chelation and played a vital role in calcium chelation. 28 The carboxylic acid groups on the side chain of the biopeptide derived from the acidic amino acids Asp and Glu, which affect the local charge density and provided a favorable environment for calcium binding. 11 The content of leucine (Leu) has an important effect on calcium binding ability.…”