Caseinophosphopeptides cytoprotect human gastric epithelium cells against the injury induced by zinc oxide nanoparticles

Wang, Yanli; Yuan, Lulu; Yao, Chenjie; Ding, Lin; Li, Chenchen; Fang, Jie; Wu, Minghong

doi:10.1039/c4ra05112b

Cited by 7 publications

(4 citation statements)

References 35 publications

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“…30 Since cytotoxicity is closely related to the oxidative stress in cells, we measured the level of ROS (reactive oxygen species) in GES-1. 31 The results in Fig. 4a show that GES-1 exposed to 15 mg L −1 ZnO NPs and 300 mg L −1 Vc for 24 h, resulted in a significant increase in the generation of ROS and there was no generation change of the ROS in GES-1 treated with 300 mg L −1 Vc or 15 mg L −1 ZnO NPs.…”

Section: Cytotoxicity Studymentioning

confidence: 87%

A combined toxicity study of zinc oxide nanoparticles and vitamin C in food additives

et al. 2014

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At present, safety evaluation standards for nanofood additives are made based on the toxic effects of a single additive. Since the size, surface properties and chemical nature influence the toxicity of nanomaterials, the toxicity may have dramatically changed when nanomaterials are used as food additives in a complex system. Herein, we investigated the combined toxicity of zinc oxide nanoparticles (ZnO NPs) and vitamin C (Vc, ascorbic acid). The results showed that Vc increased the cytotoxicity significantly compared with that of the ZnO only NPs. When the cells were exposed to ZnO NPs at a concentration less than 15 mg L(-1), or to Vc at a concentration less than 300 mg L(-1), there was no significant cytotoxicity, both in the case of gastric epithelial cell line (GES-1) and neural stem cells (NSCs). However, when 15 mg L(-1) of ZnO NPs and 300 mg L(-1) of Vc were introduced to cells together, the cell viability decreased sharply indicating significant cytotoxicity. Moreover, the significant increase in toxicity was also shown in the in vivo experiments. The dose of the ZnO NPs and Vc used in the in vivo study was calculated according to the state of food and nutrition enhancer standard. After repeated oral exposure to ZnO NPs plus Vc, the injury of the liver and kidneys in mice has been indicated by the change of these indices. These findings demonstrate that the synergistic toxicity presented in a complex system is essential for the toxicological evaluation and safety assessment of nanofood.

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Section: Cytotoxicity Studymentioning

confidence: 87%

A combined toxicity study of zinc oxide nanoparticles and vitamin C in food additives

et al. 2014

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“…However, another type of food additive, casein phosphopeptides (CPP), showed significant cytoprotective effects against cytotoxicity induced by single ZnO NPs (Fig. 5c-h) [135]. The cytotoxicity of TiO2 NPs seems far less affected in a complex system containing Vc or CPP.…”

Section: Combined Toxicity Of Monps In Food Additivesmentioning

confidence: 95%

Toxic effects of metal oxide nanoparticles and their underlying mechanisms

et al. 2017

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Nanomaterials have attracted considerable interest owing to their unique physicochemical properties. The wide application of nanomaterials has raised many concerns about their potential risks to human health and the environment. Metal oxide nanoparticles (MONPs), one of the main members of nanomaterials, have been applied in various fields, such as food, medicine, cosmetics, and sensors. This review highlights the bio-toxic effects of widely applied MONPs and their underlying mechanisms. Two main underlying toxicity mechanisms, reactive oxygen species (ROS)-and non-ROS-mediated toxicities, of MONPs have been widely accepted. ROS activates oxidative stress, which leads to lipid peroxidation and cell membrane damage. In addition, ROS can trigger the apoptotic pathway by activating caspase-9 and-3. Non-ROS-mediated toxicity mechanism includes the effect of released ions, excessive accumulation of NPs on the cell surface, and combination of NPs with specific death receptors. Furthermore, the combined toxicity evaluation of some MONPs is also discussed. Toxicity may dramatically change when nanomaterials are used in a combined system because the characteristics of NPs that play a key role in their toxicity such as size, surface properties, and chemical nature in the complex system are different from the pristine NPs.

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“…They also investigated the combined toxicity of ZnO NPs and a panel of polyphenols . In our previous studies, we found that the toxicity of ZnO NPs will significantly increase when ZnO NPs are exposed to a certain concentration of vitamin C (VC), while coincubation with casein phosphatase will reduce its toxicity . In this paper, we plan to expand the discussion on the physicochemical property and biological safety of ZnO NPs in multicomponent food system.…”

Section: Introductionmentioning

confidence: 99%

Safety Issue of Changed Nanotoxicity of Zinc Oxide Nanoparticles in the Multicomponent System

Huang

Ding

et al. 2019

Part & Part Syst Charact

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When nanomaterials are exposed to complex systems, such as food, they may cause significant changes in physical and chemical properties and even toxicity. The toxicity evaluation of complex systems is urgent. Unfortunately, so far, there is no database established about the toxicity changes of nanoparticles in composite systems. In this paper, the changes and toxicity mechanism of zinc oxide nanoparticles (ZnO NPs) in a composite system are studied. The results show that the dissolution of zinc ions (Zn2+) in acidic systems (vitamin C, tartaric acid, or citric acid) increases the toxicity of ZnO NPs. However, the toxicity of ZnO NPs is reduced by the complexation effect with Zn2+ in phosphoric acid, phosphate, and glutathione systems. The influence of titanium dioxide nanoparticles (TiO2 NPs) on the toxicity of ZnO NPs depends on size and surface properties. In brief, the intracellular Zn2+ homeostasis level is the decisive factor in determining the toxicity change in complex systems. The results indicate that the toxicity changes are very different in combined systems, which may have potential food safety issues, especially for unstable nanoparticles.

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Caseinophosphopeptides cytoprotect human gastric epithelium cells against the injury induced by zinc oxide nanoparticles

Abstract: Caseinophosphopeptides can protect GES-1 cells against the zinc oxide nanoparticles induced injury through the down-regulation of oxidative stress.

Cited by 7 publications

References 35 publications

A combined toxicity study of zinc oxide nanoparticles and vitamin C in food additives

A combined toxicity study of zinc oxide nanoparticles and vitamin C in food additives

Toxic effects of metal oxide nanoparticles and their underlying mechanisms

Safety Issue of Changed Nanotoxicity of Zinc Oxide Nanoparticles in the Multicomponent System

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