Caspase-14: a new player in cytotrophoblast differentiation

White, Lloyd; Dharmarajan, Arun; Charles, Adrian

doi:10.1016/s1472-6483(10)60871-1

Cited by 16 publications

(8 citation statements)

References 34 publications

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“…In particular, initiator caspase 8 and caspase 14 are involved in this process [56,57]. Adiponectin has been described as a pro-apoptotic factor in many cell types, including breast cancer cells [58] and endometrial cells [17].…”

Section: Discussionmentioning

confidence: 99%

Adiponectin promotes syncytialisation of BeWo cell line and primary trophoblast cells

Benaîtreau

Santos

Leneveu

et al. 2010

Reprod Biol Endocrinol

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BackgroundIn human pregnancy, a correct placentation depends on trophoblast proliferation, differentiation, migration and invasion. These processes are highly regulated by placental hormones, growth factors and cytokines. Recently, we have shown that adiponectin, an adipokine, has anti-proliferative effects on trophoblastic cells. Here, we complete this study by demonstrating that adiponectin modulates BeWo and human villous cytotrophoblast cell differentiation.ResultsWe showed that hCG secretion was up-regulated by adiponectin treatment in both BeWo cells and human cytotrophoblasts from very early placentas (5-6 weeks). The expression of two trophoblast differentiation markers, leptin and syncytin 2, was also up-regulated by adiponectin in BeWo cells. Moreover, adiponectin treatment induced a loss of E-cadherin staining in these cells. In parallel, we demonstrated that AdipoR1 and AdipoR2 are up-regulated during forskolin induced BeWo cell differentiation, reinforcing the role of adiponectin in trophoblast syncytialization. SiRNA mediated down-regulation of AdipoR1 and AdipoR2 was used to demonstrate that adiponectin effects on differentiation were essentially mediated by these receptors. Finally, using a specific inhibitor, we demonstrated that the PKA signalling pathway could be one pathway involved in adiponectin effects on trophoblast differentiation.ConclusionAdiponectin enhances the differentiation process of trophoblast cells and could thus be involved in functional syncytiotrophoblast formation.

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Section: Discussionmentioning

confidence: 99%

Adiponectin promotes syncytialisation of BeWo cell line and primary trophoblast cells

Benaîtreau

Santos

Leneveu

et al. 2010

Reprod Biol Endocrinol

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“…Quantification of cell death was determined using the JC-1 technique [43] (Invitrogen). At the onset of cell death, the mitochondrial membrane is rapidly depolarized.…”

Section: Methodsmentioning

confidence: 99%

Secreted frizzled-related protein 4 expression is positively associated with responsiveness to Cisplatin of ovarian cancer cell lines in vitro and with lower tumour grade in mucinous ovarian cancers

et al. 2012

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BackgroundOvarian cancer is one of the most lethal malignancies in women, as it is frequently detected at an advanced stage, and cancers often become refractory to chemotherapy. Evidence suggests that dysregulation of pro-apoptotic genes plays a key role in the onset of chemoresistance. The secreted Frizzled-Related Protein (sFRP) family is pro-apoptotic and also a negative modulator of the Wnt signalling cascade. Studies have demonstrated that the re-expression of sFRPs, in particular sFRP4, is associated with a better prognosis, and that experimentally induced expression results in cell death.ResultsIn vitro experimental models determined that sFRP4 was differentially expressed in chemosensitive (A2780) and chemoresistant (A2780 ADR and A2780 Cis) ovarian cell lines, with chemosensitive cells expressing significantly higher levels of sFRP4. Transfection of the chemoresistant cell lines with sFRP4 significantly increased their sensitivity to chemotherapy. Conversely, silencing of sFRP4 expression in the chemosensitive cell line resulted in a corresponding increase in chemoresistance. Comparison of sFRP4 expression in tumour biopsies revealed a positive trend between sFRP4 expression and tumour grade, with mucinous cyst adenocarcinomas exhibiting significantly decreased sFRP4 levels compared to mucinous borderline tumours.ConclusionsThis study indicates a role for sFRP4 as a predictive marker of chemosensitivity in ovarian cancer and suggests that this pathway may be worth exploiting for novel therapies.

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“…29 At the onset of apoptosis the mitochondrial membrane is rapidly depolarized. When the mitochondrial membrane is polarized the JC-1 dye (5,5Ј,6,6Ј-tetrachloro-1,1Ј,3,3Ј-tetraethyl-benzimidazolylcarbocyanine iodide) aggregates and fluoresces red.…”

Section: Jc-1 Assaymentioning

confidence: 99%

Secreted Frizzled-Related Protein 4

Muley

Majumder

Kolluru

et al. 2010

The American Journal of Pathology

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Wnt signaling is involved in developmental processes , cell proliferation , and cell migration. Secreted frizzled-related protein 4 (sFRP4) has been demonstrated to be a Wnt antagonist; however , its effects on endothelial cell migration and angiogenesis have not yet been reported. Using various in vitro assays, we show that sFRP4 inhibits endothelial cell migration and the development of sprouts and pseudopodia as well as disrupts the stability of endothelial rings in addition to inhibiting proliferation. sFRP4 interfered with endothelial cell functions by antagonizing the canonical Wnt/␤-catenin signaling pathway and the Wnt/planar cell polarity pathway. Furthermore, sFRP4 blocked the effect of vascular endothelial growth factor on endothelial cells. sFRP4 also selectively induced apoptotic events in endothelial cells by increasing cellular levels of reactive oxygen species. In vivo assays demonstrated a reduction in vascularity after sFRP4 treatment. Most importantly , sFRP4 restricted tumor growth in mice by interfering with endothelial cell function. The data demonstrate sFRP4 to be a potent angiogenesis inhibitor that warrants further investigation as a therapeutic agent in the control of angiogenesis-associated pathology.

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Caspase-14: a new player in cytotrophoblast differentiation

Cited by 16 publications

References 34 publications

Adiponectin promotes syncytialisation of BeWo cell line and primary trophoblast cells

Adiponectin promotes syncytialisation of BeWo cell line and primary trophoblast cells

Secreted frizzled-related protein 4 expression is positively associated with responsiveness to Cisplatin of ovarian cancer cell lines in vitro and with lower tumour grade in mucinous ovarian cancers

Secreted Frizzled-Related Protein 4

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