Caspase-14 is an anti-apoptotic protein targeting apoptosis-inducing factor in lung adenocarcinomas

Chow, Kuan‐Chih

doi:10.3892/or.2011.1292

Cited by 12 publications

(6 citation statements)

References 26 publications

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“…However, LK2 cell secretion experiments with serum-free medium concluded that serum EV with CHL1–caspase 14 BiEV targeted in this study may display caspase 14 on the EV surface during the process of EV formation in LC cells. Similar to SLC4A1 in mice, the high expression levels of caspase 14 itself has been observed in cancer cells (Fang et al, 2011; Koenig et al, 2005; Krajewska et al, 2005), but expression of caspase 14 in healthy EVs and cancer EVs exhibited no significant differences as a whole (Figure 7E). This also demonstrates that fluorescein-labeled caspase 14 should be quantified in BiEV analysis.…”

Section: Discussionsupporting

confidence: 54%

Bimolecule detection for Extracellular Vesicle Screening

Kaneda

Ida²,

Kuwahara

et al. 2020

Preprint

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Extracellular vesicles (EVs) have been investigated for use in clinical testing in recent years. Specific EV surface proteins provide distinguishing characteristics, but are insufficient for more detailed classification of EVs. Here, we suggest a novel “Bimolecular surface antigen expressed in EV” (BiEV) as a potential indicator for more efficient EV screening. A BiEV can be identified using a proximity labeling method applicable to EV membrane proteins. We examined the screening of BiEV in cancer cell-secreted EV included in serum EVs from a model mouse for lung cancer, showing that the CHL1-SLC4A1 BiEV was a significant candidate. ELISA quantification of CHL1-SLC4A1 BiEV appeared to suggest a potential for cancer screening of these mice. Using the same protocols, we found that CHL1-caspase 14 BiEV was significantly elevated in lung cancer patients. A BiEV strategy may be able to make a contribution to more effective EV screening, resulting in novel clinical applications.

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Section: Discussionsupporting

confidence: 54%

Bimolecule detection for Extracellular Vesicle Screening

Kaneda

Ida²,

Kuwahara

et al. 2020

Preprint

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“…RO T1D Tregs overexpressed pro-apoptotic Bcl2-family genes (>2299-fold Bcl2L10 and >25-fold Bax) Figure 4B), as well as anti-apoptotic genes (>266-fold Mcl1 and >331-fold Bag1) and caspase 14, which might act as an anti-apoptotic molecule due to suppressive nature of its interaction with apoptosis-inducing factor [55]. This suggests desperate attempts Tregs make to recover and counteract already initiated apoptosis process.…”

Section: Resultsmentioning

confidence: 97%

Interaction between Treg Apoptosis Pathways, Treg Function and HLA Risk Evolves during Type 1 Diabetes Pathogenesis

Glišić

Jailwala

2012

PLoS ONE

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We have previously reported increased apoptosis of regulatory T cells (Tregs) in recent-onset Type 1 Diabetes subjects (RO T1D) in the honeymoon phase and in multiple autoantibody-positive (Ab+) subjects, some of which are developing T1D. We have also reported that increased Treg apoptosis was associated with High HLA risk and that it subsided with cessation of honeymoon period. In this report, we present results generated using genetics, genomics, functional cell-based assays and flow cytometry to assess cellular changes at the T-cell level during T1D pathogenesis. We measured ex vivo Treg apoptosis and Treg function, surface markers expression, expression of HLA class II genes, the influence of HLA risk on Treg apoptosis and function, and evaluated contribution of genes reported to be involved in the apoptosis process. This integrated comprehensive approach uncovered important information that can serve as a basis for future studies aimed to modulate Treg cell responsiveness to apoptotic signals in autoimmunity. For example, T1D will progress in those subjects where increased Treg apoptosis is accompanied with decreased Treg function. Furthermore, Tregs from High HLA risk healthy controls had increased Treg apoptosis levels and overexpressed FADD but not Fas/FasL. Tregs from RO T1D subjects in the honeymoon phase were primarily dying through withdrawal of growth hormones with contribution of oxidative stress, mitochondrial apoptotic pathways, and employment of TNF-receptor family members. Ab+ subjects, however, expressed high inflammation level, which probably contributed to Treg apoptosis, although other apoptotic pathways were also activated: withdrawal of growth hormones, oxidative stress, mitochondrial apoptosis and Fas/FasL apoptotic pathways. The value of these results lie in potentially different preventive treatment subjects would receive depending on disease progression stage when treated.

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“…Furthermore, CASP14 has been reported to be an anti-apoptotic protein, bound with cytochrome c to reduce cisplatin sensitivity in vitro . As CASPs and cytochrome c have recently emerged as innovative targets for antitumor drugs, including camptothecin, adriamycin and cisplatin with aim to destabilize the mitochondrial membrane and activate procaspases, CASP14 may inhibit drug-induced DNA fragmentation and cell death associated with increased chemoresistance (44).…”

Section: Discussionmentioning

confidence: 99%

Prognostic significance of mRNA expression of CASPs in gastric cancer

Wang

et al. 2019

Oncol Lett

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Current studies suggest that the cysteinyl aspartate specific proteinase (caspase/CASP) family may be closely associated with apoptosis. Scientists have suggested that caspases may be a key to the development of more effective anti-cancer therapies. However, the prognostic value of CASP expression in gastric cancer (GC) remains unclear. Using a Kaplan-Meier plotter online database, the predictive prognostic significance of the expression of 12 CASPs genes (CASP1, CASP2, CASP3, CASP4, CASP5, CASP6, CASP7, CASP8, CASP9, CASP10, CASP12 and CASP14) to overall survival (OS) in different clinicopathological features, including Lauren classification, pathological stages, therapies employed and differentiation in gastric cancer patients was explored. The present study revealed that higher CASP1, 2, 3, 4, 5, 6, 7 and 8 mRNA expression was associated with better OS, whereas higher expression of CASP9, 10, 12 and 14 showed an unfavorable OS in all GC patients. Moreover, CASP1 to 8 were all associated with favorable OS in intestinal type and diffuse type classified by Lauren classification. Therefore, the results of the present study suggested that the CASP family may function as new prognostic indicators in GC and may be helpful in making treatment decisions.

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Caspase-14 is an anti-apoptotic protein targeting apoptosis-inducing factor in lung adenocarcinomas

Cited by 12 publications

References 26 publications

Bimolecule detection for Extracellular Vesicle Screening

Bimolecule detection for Extracellular Vesicle Screening

Interaction between Treg Apoptosis Pathways, Treg Function and HLA Risk Evolves during Type 1 Diabetes Pathogenesis

Prognostic significance of mRNA expression of CASPs in gastric cancer

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