Caspase-3 and heat shock protein-70 in rat liver treated with aflatoxin B1: effect of melatonin

Meki, Abdel-Raheim M.A.; Esmail, Emade El-Dein F; Hussein, Ahmed; Hassanein, Hamdy

doi:10.1016/j.toxicon.2003.10.026

Cited by 97 publications

(52 citation statements)

References 49 publications

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“…In the present study, hepatic lipid peroxidation (LP) was significantly increased while the hepatic antioxidant enzymes GPX, SOD and catalase activities were significantly decreased in AFB 1 -treated rats compared with those in the control group. These results are in agreement with the findings of Meki et al (2004) who reported that the increased LP level with the concomitant decrease in antioxidant enzyme activities were the most pronounced markers for AFB 1 toxicity and carcinogenicity.…”

Section: Discussionsupporting

confidence: 93%

Prevention of aflatoxin B1-initiated hepatotoxicity in rat by marine algae extracts

2006

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Chemoprevention by extracts of Laurencia obtusa (E1) and Caulerpa prolifera (E2) collected from the Egyptian coast of the Red Sea against aflatoxin B(1) (AFB(1))-initiated hepatotoxicity in female Sprague-Dawley rats has been studied. Animals were fed aflatoxin-contaminated diet (3 mg kg(-1) diet) for 6 days then treated orally with pure aflatoxin B(1) (AFB(1)) (200 microg kg(-1) b.w.) for 4 days either in combination with or before E1 or E2 administration (50 mg kg(-1) b.w.). AFB(1) resulted in a signicant increase in serum alpha fetoprotein, carcinoembryonic antigen, tumor necrosis factor alpha, nitric oxide, interleukin-1alpha, procollagen III and lipid peroxidation level in the liver. It caused a signicant decrease in food intake, body weight, serum leptin, the activities of glutathione peroxidase, superoxide dismutase and DNA and RNA concentrations in the liver. Cotreatment with AFB(1) and E1 or E2 resulted in an obvious improvement in all tested parameters. Noteworthy, E2 was more effective than E1 in the protection against AFB(1)-induced hepatotoxicity.

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Section: Discussionsupporting

confidence: 93%

Prevention of aflatoxin B1-initiated hepatotoxicity in rat by marine algae extracts

2006

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“…Decrease in the activities of antioxidants enzymes as observed in this study is prominent in AFB 1 carcinogenesis/toxicity (Souza et al 1999;Meki et al 2001Meki et al , 2004Ajiboye et al 2013;Adeleye et al 2014). The AFB 1 -mediated decrease in the activities of these enzymes in rat hepatocytes, attributed to overwhelmed antioxidant enzymes, indicates oxidative stress arising from ROS (superoxide anion and nitric oxide) generated.…”

Section: Oxidative Stresssupporting

confidence: 64%

“…The attenuation of AFB 1 -mediated decrease in the activities of these enzymes (SOD, CAT, GSH-Px and GSH-red) in rat hepatocytes shows the capability of lophirones B and C to prevent oxidative stress possibly by enhancing Nrf-2 expression (Ajiboye et al 2014c). Meki et al (2004) reported similar reversal of AFB 1 -mediated decrease in antioxidant enzymes following treatment of rats with melatonin. Reduction in the hepatocytes GSH level may be linked to overwhelmed antioxidant enzymes.…”

Section: Oxidative Stressmentioning

confidence: 67%

Lophirones B and C prevent aflatoxin B₁-induced oxidative stress and DNA fragmentation in rat hepatocytes

Ajiboye

Yakubu

Oladiji

2016

Pharmaceutical Biology

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Context Despite the reported anticarcinogenic activity of lophirones B and C, no scientific information exists for its activity in rat hepatocytes. Objective Effect of lophirones B and C on aflatoxin B 1 (AFB 1 )-induced oxidative stress, and DNA fragmentation in rat hepatocytes was investigated. Materials and methods Wistar rat hepatocytes were incubated with lophirones B and C (1 mg/mL) or sylimarin (1 mg/mL) in the presence or absence of AFB 1 . For an in vivo study, rats were orally administered with lophirones B and C, and/or AFB 1 (20 mg/d) for 9 weeks. Results Lophirones B and C lowered AFB 1 -mediated increase in nitric oxide, superoxide anion radicals, caspase-3 and fragmented DNA. Lophirones B and C attenuated AFB 1 -mediated decrease in superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase, and reduced glutathione. Also, lophirones B and C attenuated AFB 1 -mediated increase in conjugated dienes, lipid hydroperoxides and malondialdehyde in rat hepatocytes. Furthermore, AFB 1 -mediated alterations in alkaline phosphatase, alanine aminotransferase, aspartate aminotransferase, albumin, total bilirubin and globulin in rat serum were significantly annulled in lophirones B and C-treated rats. Conclusion This study revealed that lophirones B and C prevented AFB 1 -induced oxidative damage in rat hepatocytes. ARTICLE HISTORY

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“…The GPx perform its activity in cytosolic fraction whereas Catalase is performing its activity in microbodies. These results are comparative with the results of Meki et al (2004), who reported significant reduction in GPx and GR activities in liver after 10 days of AFB 1 treatment.…”

Section: Advances In Animal and Veterinary Sciences May 2016 | Volumesupporting

confidence: 60%

Does Bougainvillea spectabilis protect Swiss Albino Mice from Aflatoxin-induced Hepatotoxicity?

Mishra¹,

Tandon²,

Dhama³

et al. 2016

Adv. Anim. Vet. Sci.

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| We have investigated the protective effect of oral administration of the ethanolic extract of Bougainvillea spectabilis leaves against aflatoxin B 1 (AFB 1 ) induced hepatic injuries among male Swiss albino mice. AFB 1 exposure has significantly increased (P< 0.05) the lipid peroxidase activity and decreased the activities of various antioxidant enzymes viz. superoxide dismutase (57.58%), catalase (24.14%), glutathione peroxidase (44.21%), glutathione S-transferase (44.07%) and glutathione reductase (13.51%). It also declined the level of ascorbic acid, reduced glutathione and protein contents by 47%, 34% and 24% respectively. It markedly decreased lipid peroxidation (TBARS level) with concomitant stimulation (P < 0.05) of antioxidants (enzymatic and non -enzymatic both). HPTLC analysis revealed availability of sufficient amount of flavonoids, alkaloids and phenolic compounds in the extract of B. spectabilis leaves might be responsible for the improved efficiency of antioxidant system and subsequent protection from aflatoxicity.

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Caspase-3 and heat shock protein-70 in rat liver treated with aflatoxin B1: effect of melatonin

Cited by 97 publications

References 49 publications

Prevention of aflatoxin B1-initiated hepatotoxicity in rat by marine algae extracts

Prevention of aflatoxin B1-initiated hepatotoxicity in rat by marine algae extracts

Lophirones B and C prevent aflatoxin B₁-induced oxidative stress and DNA fragmentation in rat hepatocytes

Does Bougainvillea spectabilis protect Swiss Albino Mice from Aflatoxin-induced Hepatotoxicity?

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Caspase-3 and heat shock protein-70 in rat liver treated with aflatoxin B1: effect of melatonin

Cited by 97 publications

References 49 publications

Prevention of aflatoxin B1-initiated hepatotoxicity in rat by marine algae extracts

Prevention of aflatoxin B1-initiated hepatotoxicity in rat by marine algae extracts

Lophirones B and C prevent aflatoxin B1-induced oxidative stress and DNA fragmentation in rat hepatocytes

Does Bougainvillea spectabilis protect Swiss Albino Mice from Aflatoxin-induced Hepatotoxicity?

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Lophirones B and C prevent aflatoxin B₁-induced oxidative stress and DNA fragmentation in rat hepatocytes