Caspase 3 may participate in the anti-tumor immunity of dendritic cells

Liu, Jinqiang; Wang, Fei; Yin, Dandan; Zhang, Hongwei; Feng, Fan

doi:10.1016/j.bbrc.2019.02.081

Cited by 9 publications

(4 citation statements)

References 27 publications

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“…Wang et al reported that CASP3 can specifically cleave and activate GSDME and cause pyroptosis 19 . Dendritic cells, the sentinel of immune system, is the most effective specific antigen‐presenting cells, Liu et al noted that CASP3 gene overexpression could accelerate maturation and enhance anti‐tumor capability of dendritic cells, 54 and B cell differentiation was associated with the change in expression of CASP3 55 . Besides, a recent study suggested that BCR signaling pathway may be involved in periodontitis in Down syndrome 56 .…”

Section: Discussionmentioning

confidence: 99%

Pyroptosis may play a crucial role in modifications of the immune microenvironment in periodontitis

Chen

Wang

et al. 2022

J of Periodontal Research

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Background and objective Published studies proved that both pyroptosis and periodontitis owned a substantial relationship with immunity, and recent research revealed a solid correlation between periodontitis and pyroptosis. While abundant findings have confirmed pyroptosis has a strong impact on the tumor microenvironment, the function of pyroptosis in influencing the periodontitis immune microenvironment remains poorly understood. Thus, we aimed to identify pyroptosis‐related genes whose expression signature can well discriminate periodontitis from healthy controls and to comprehend the role of pyroptosis in the periodontitis immune microenvironment. Materials and methods The periodontitis‐related datasets were acquired from the Gene Expression Omnibus (GEO) database. A series of bioinformatics analyses were conducted to investigate the underlying mechanism of pyroptosis in the periodontitis immune microenvironment. Infiltrating immunocytes, immunological reaction gene sets, and the human leukocyte antigen (HLA) gene were all investigated as potential linkages between periodontitis immune microenvironment and pyroptosis. Results Twenty‐one pyroptosis‐related genes were dysregulated. A four‐mRNA combined classification model was constructed, and the receiver operating characteristic (ROC) curve analysis demonstrated its prominent classification capabilities. Subsequently, the mRNA levels of the four hub markers (CYCS, CASP3, NOD2, CHMP4B) were validated by quantitative real‐time PCR (qRT‐PCR). The correlation coefficients between each hub gene and immune characteristics were calculated, and CASP3 exhibited the most significant correlations with the immune characteristics. Furthermore, distinct pyroptosis‐related expression patterns were revealed, along with immunological features of each pattern. Afterward, we discovered 1868 pyroptosis phenotype‐related genes, 134 of which were related to immunity. According to the functional enrichment analysis, these 134 genes were closely related to cytokine signaling in immune system, and defense response. Finally, a co‐expression network was constructed via the 1868 gene expression profiles. Conclusion Four hub mRNAs (CYCS, CASP3, NOD2, and CHMP4B) formed a classification model and concomitant results revealed the crucial role of pyroptosis in the periodontitis immune microenvironment, providing fresh insights into the etiopathogenesis of periodontitis and potential immunotherapy.

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Section: Discussionmentioning

confidence: 99%

Pyroptosis may play a crucial role in modifications of the immune microenvironment in periodontitis

Chen

Wang

et al. 2022

J of Periodontal Research

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“…Casp3 was found to be the central node in network of targets of the down-regulated miRNAs. Casp3, reported to be induced in maturing DCs, may be a signature of mDCs (Jin et al, 2010) and its overexpression has been found to promote DC maturation and T cell activation (Liu et al, 2019).…”

Section: Discussionmentioning

confidence: 99%

Identification and functional characterization of maturation-dependent changes in dendritic cell exosome-shuttle targetome

Ganguly

2024

Preprint

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Dendritic cells (DCs) are the most professional antigen-presenting cells, which undergo a hallmark transition from an immature to a mature state. DCs release high levels of exosomes (DCEs), containing miRNAs, which orchestrate their tolerogenic or immunogenic functions. This study aimed to identify the exosomes-shuttle miRNAs that are differentially expressed between the mature and immature states of DCs, and to assign functional enrichments to the targets of these miRNAs. A GEO data series comparing miRNA expression in mature and immature DCEs was analyzed and all miRNAs significantly dysregulated between mature and immature states of DCEs were identified. The interactions and targets were mapped separately for the upregulated and down-regulated miRNAs, and interaction networks and functional enrichments of the targets were generated and visualized. 24 miRNAs were found upregulated and 19 miRNAs were found down-regulated in the exosomes of mature DCs over exosomes of immature DCs with 1949 and 1186 targets involved in 131 and 32 pathways, respectively. Further, the functional enrichment of the targets revealed miRNA-targeted changes in expression of biomolecules involved in cytoskeletal remodeling and energy metabolism as key maturation-dependent processes. The results present salient miRNA signatures for identifying DC maturation state and uncover miRNA targets that may serve as therapeutic options in the treatment of various immune dysfunctions.

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“…Cytochrome c (Cyto c) is released and forms a complex with the cytoplasmic apoptotic protease activator Apaf‐1, which recruits and activates procaspase 9, activates caspase 3 and shears PARP. After these changes, the cells are allowed to enter apoptosis . Firstly, we detected the changes in Bax and Bcl‐2 mRNA expression by PCR analysis.…”

Section: Discussionmentioning

confidence: 99%

“…After these changes, the cells are allowed to enter apoptosis. [39,40] Firstly, we detected the changes in Bax and Bcl-2 mRNA expression by PCR analysis. It can be seen from the histogram that Bax was on the rise and Bcl-2 was on the decline.…”

Section: Discussionmentioning

confidence: 99%

Meriolin1 induces cell cycle arrest, apoptosis, autophagy and targeting the Akt/MAPKs pathways in human neuroblastoma SH-SY5Y cells

Wang

et al. 2020

Journal of Pharmacy and Pharmacology

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Objectives Meriolins, a kind of chemical hybrid between meridianins and variolins, have lately been determined as kinase inhibitors and reportedly have antitumour activity. However, there is currently no in‐depth study for the action mechanism. This study aimed to elucidate the potentially antitumour action mechanism of Meriolin1 on human neuroblastoma (SH‐SY5Y) cells. Methods Firstly, cell viability was detected by MTT assay. Secondly, cell cycle, cell apoptosis, cell autophagy, reactive oxygen species and mitochondrial membrane potential (ΔΨm) were measured by flow cytometry. Then, cell cycle‐associated proteins, Bcl‐2 family proteins, Akt/MAPK proteins and autophagy‐associated proteins expressions were evaluated by Western blot. Bcl‐2 and Bax mRNA expressions were also evaluated by qRT‐PCR. Furthermore, cell adhesion assay and Hoechst 33258 fluorescent staining were carried out to detect the effect of Meriolin1 on cell adhesion and morphology. Finally, to gain further insight into mechanism of action of Meriolin1 to CDK protein, the molecular docking study was performed by using the CDOCKER module of DS software. Key findings Meriolin1 could exert the antitumour activity on SH‐SY5Y cells by inducing cell cycle arrest, cell autophagy, the mitochondrion‐dependent cell apoptosis and targeting the Akt/MAPKs signalling pathway. Conclusions Meriolin1 might be a promising therapeutic candidate for neuroblastoma.

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Caspase 3 may participate in the anti-tumor immunity of dendritic cells

Cited by 9 publications

References 27 publications

Pyroptosis may play a crucial role in modifications of the immune microenvironment in periodontitis

Pyroptosis may play a crucial role in modifications of the immune microenvironment in periodontitis

Identification and functional characterization of maturation-dependent changes in dendritic cell exosome-shuttle targetome

Meriolin1 induces cell cycle arrest, apoptosis, autophagy and targeting the Akt/MAPKs pathways in human neuroblastoma SH-SY5Y cells

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