Caspase and nm23: Apoptosis genes linked to the antibacterial response of the Chinese mitten crab

Jin, Xing‐Kun; Li, Weiwei; Wang, Qun

doi:10.4161/bbug.2.3.15046

Cited by 4 publications

(3 citation statements)

References 21 publications

(22 reference statements)

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“…Profiles 16 (55, 10.2%) exhibited the next highest number of DEGs upon salt stress, with transcripts that were upregulated immediately at 12 h following the salinity stress, which were then downregulated after 24 h. Among these, the V‐type proton ATPase and heat shock protein were found in Profile 7, which were associated with ion transport and stress resistance and played an important role in salinity adaptation (Fu et al, 2013; Romano & Zeng, 2012). In addition, two caspase family genes in Profile 16 were also identified and associated with apoptosis (Jin et al, 2011). The results suggest that ion transport, stress resistance, and apoptosis were involved in the special salinity adaptation in P .…”

Section: Resultsmentioning

confidence: 99%

A chromosome‐level genome of Portunus trituberculatus provides insights into its evolution, salinity adaptation and sex determination

et al. 2021

Molecular Ecology Resources

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Portunus trituberculatus (Crustacea: Decapoda: Brachyura), commonly known as the swimming crab, is of major ecological importance, as well as being important to the fisheries industry. P. trituberculatus is also an important farmed species in China due to its rapid growth rate and high economic value. Here, we report the genome sequence of the swimming crab, which was assembled at the chromosome scale, covering ~1.2 Gb, with 79.99% of the scaffold sequences assembled into 53 chromosomes. The contig and scaffold N50 values were 108.7 kb and 15.6 Mb, respectively, with 19,981 protein‐coding genes. Based on comparative genomic analyses of crabs and shrimps, the C2H2 zinc finger protein family was found to be the only gene family expanded in crab genomes, suggesting it was closely related to the evolution of crabs. The combination of transcriptome and bulked segregant analysis provided insights into the genetic basis of salinity adaptation and rapid growth in P. trituberculatus. In addition, the specific region of the Y chromosome was located for the first time in the genome of P. trituberculatus, and three genes were preliminarily identified as candidate genes for sex determination in this region. Decoding the swimming crab genome not only provides a valuable genomic resource for further biological and evolutionary studies, but is also useful for molecular breeding of swimming crabs.

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Section: Resultsmentioning

confidence: 99%

A chromosome‐level genome of Portunus trituberculatus provides insights into its evolution, salinity adaptation and sex determination

et al. 2021

Molecular Ecology Resources

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“…Apoptosis is the core regulatory characteristic of the immune system, as well as the most common form of immunological cell death (Jin, Li, He, et al, 2011). Previous studies have indicated that during the growth of mitten crabs, some genes involved in the apoptosis pathway, like caspase, participate in the resistance to adverse environments (Jin, Li, Wang et al, 2011; Qu et al, 2019). Additionally, two apoptosis genes, caspase and nm23, have been isolated from the Chinese mitten crab, both of which play important roles in the innate immune system of mitten crabs (Jin, Li, He, et al, 2011).…”

Section: Discussionmentioning

confidence: 99%

Molecular dominance investigation of large‐sized Chinese mitten crab ( Eriocheir sinensis ) parents based on the male accessory gland transcriptome

Xuan

Zhao

et al. 2021

Aquaculture Research

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Germplasm degradation is one of the important issues for the healthy development of Chinese mitten crab (Eriocheir sinensis) industry. Nowadays, many farming practices have evidenced the seedling superiority of large-sized parents (≥200 g male, ≥150 g female), but the underlying molecular mechanisms remain unclear. This study investigated the advantage of large-sized male parents in E. sinensis breeding at the molecular level. Accessory glands were obtained from three large-sized male crabs and three medium-sized male crabs for transcriptome sequencing. A total of 8000 differentially expressed genes (DEGs, 2773 up-regulated and 5227 down-regulated) were identified between the large-and medium-sized groups. According to previous studies, 324 genes involved in 12 reproductive, immune response and growth pathways were identified. A total of 38 common genes were selected, and 11 were verified to have consistent expression trends in the sequencing data after quantitative real-time PCR validation. These validated genes, such as TRINITY_DN1908_c0_g2,

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“…A recent transcriptomic analysis showed that the growth inhibition and survival rate reduction induced by various factors (such as hepatopancreatic necrosis disease) were signi cantly correlated with apoptosis in the development of E. sinensis [36]. Importantly, genes such as nm23 and caspase in the apoptosis pathway participate in resistance to adverse environments during the growth of E. sinensis individuals [37,38]. Thus, large-sized parents of E. sinensis might display a strong adverse environment resistance via gene expression in apoptosis, which further bene ts the improvement of individual survival rates and economic value.…”

Section: Discussionmentioning

confidence: 99%

Molecular dominance investigation for large-sized parents of Chinese Mitten Crab ( Eriocheir sinensis ) based on ovarian transcriptome

Xuan

Wang

et al. 2020

Preprint

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Background: Germplasm degradation is one of the important issues for the healthy development of Chinese Mitten Crab (Eriocheir sinensis) industry. Nowadays, nursery units and crab farmers have realized the seedling superiority of large-sized parents (≥200g male, ≥150g female), but the underlying molecular mechanisms remain unclear. Here we investigated the advantage of breeding with large-sized parents based on ovarian transcriptome.Results: RNA isolation and transcriptome sequencing were performed using ovarian tissue obtained from three large-sized female E. sinensis and three medium-sized female E. sinensis. The differentially expressed genes (DEGs) were investigated between two groups, followed by the exploration of common DEGs (co-DEGs) among the DEGs in the current study and published genes for growth, reproduction, and immune response pathways. Quantitative real-time polymerase chain reaction (qRT-PCR) was performed to validate all co-DEGs. Finally, a pathway enrichment analysis was performed to investigate the potential pathways associated with the validated co-DEGs. A total of 5,307 up-regulated genes and 3,465 down-regulated genes were investigated between the two groups, of which 43 co-DEGs were explored when all the DEGs in the current study were integrated with 338 genes in 15 reproductive, immune, and growth pathways. A total of nine co-DEGs, including TRINITY_DN13931_c0_g1, TRINITY_DN1908_c0_g2, and TRINITY_DN6686_c0_g1, were enrolled for further study after qRT-PCR validation. These validated co-DEGs were mainly enriched in pathways such as apoptosis, insulin signaling, and mTOR signaling. Conclusion: Our original work might lay an important molecular foundation for further exploring the advantages of using large-sized parents for E. sinensis breeding in the future. Based on the comparative analysis of gene expression, the advantages of breeding large-sized female E. sinensis parents at the molecular level, such as growth, reproduction, and immunity, were analyzed for the first time. The apoptosis pathways, insulin signaling pathways, and mTOR signaling pathway might be involved in the advantage of breeding large-sized female E. sinensis parents.

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Caspase and nm23: Apoptosis genes linked to the antibacterial response of the Chinese mitten crab

Cited by 4 publications

References 21 publications

A chromosome‐level genome of Portunus trituberculatus provides insights into its evolution, salinity adaptation and sex determination

A chromosome‐level genome of Portunus trituberculatus provides insights into its evolution, salinity adaptation and sex determination

Molecular dominance investigation of large‐sized Chinese mitten crab ( Eriocheir sinensis ) parents based on the male accessory gland transcriptome

Molecular dominance investigation for large-sized parents of Chinese Mitten Crab ( Eriocheir sinensis ) based on ovarian transcriptome

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Caspase and nm23: Apoptosis genes linked to the antibacterial response of the Chinese mitten crab

Cited by 4 publications

References 21 publications

A chromosome‐level genome of Portunus trituberculatus provides insights into its evolution, salinity adaptation and sex determination

A chromosome‐level genome of Portunus trituberculatus provides insights into its evolution, salinity adaptation and sex determination

Molecular dominance investigation of large‐sized Chinese mitten crab ( Eriocheir sinensis ) parents based on the male accessory gland transcriptome

Molecular dominance investigation for large-sized parents&nbsp;of Chinese Mitten Crab ( Eriocheir sinensis ) based on&nbsp;ovarian transcriptome

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Molecular dominance investigation for large-sized parents of Chinese Mitten Crab ( Eriocheir sinensis ) based on ovarian transcriptome