Casticin Induces DNA Damage and Impairs DNA Repair in Human Bladder Cancer TSGH-8301 Cells

Huang, An‐Cheng; Cheng, Yih-Dih; Huang, Li-Chi; Hsiao, Yung-Ting; Peng, Shu‐Fen; Lu, Kung‐Wen; Lien, Jin‐Cherng; Yang, Jong-Long; Lin, Tzu-Shun; Chung, Jing‐Gung

doi:10.21873/anticanres.13291

Cited by 17 publications

(19 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…After that, the cells were washed and stained with anti-Bcl-xL and -Bak (green fluorescence) followed with FITC-conjugated goat anti-mouse IgG (secondary antibody). The nucleus was stained by DAPI (blue fluorescence) and PI (red fluorescence) for double-check, and Leica TCS SP2 Confocal Spectral Microscope was used to examine photomicrography as described previously [ 26 ]. The localization of Bcl-xLor Bak in the cytosol was formed in green color based on blue and red overlapped pixels.…”

Section: Methodsmentioning

confidence: 99%

PW06 Triggered Fas-FADD to Induce Apoptotic Cell Death In Human Pancreatic Carcinoma MIA PaCa-2 Cells through the Activation of the Caspase-Mediated Pathway

Huang

Hsia

Yeh

et al. 2023

Oxidative Medicine and Cellular Longevity

Self Cite

View full text Add to dashboard Cite

Pancreatic cancer has higher incidence and mortality rates worldwide. PW06 [(E)-3-(9-ethyl-9H-carbazol-3-yl)-1-(2,5-dimethoxyphenyl) prop-2-en-1-one] is a carbazole derivative containing chalcone moiety which was designed for inhibiting tumorigenesis in human pancreatic cancer. This study is aimed at investigating PW06-induced anticancer effects in human pancreatic cancer MIA PaCa-2 cells in vitro. The results showed PW06 potent antiproliferative/cytotoxic activities and induced cell morphological changes in a human pancreatic cancer cell line (MIA PaCa-2), and these effects are concentration-dependent (IC50 is 0.43 μM). Annexin V and DAPI staining assays indicated that PW06 induced apoptotic cell death and DNA condensation. Western blotting indicated that PW06 increased the proapoptotic proteins such as Bak and Bad but decreased the antiapoptotic protein such as Bcl-2 and Bcl-xL. Moreover, PW06 increased the active form of caspase-8, caspase-9, and caspase-3, PARP, releasing cytochrome c, AIF, and Endo G from mitochondria in MIA PaCa-2 cells. Confocal laser microscopy assay also confirmed that PW06 increased Bak and decreased Bcl-xL. Also, the cells were pretreated with inhibitors of caspase-3, caspase-8, and caspase-9 and then were treated with PW06, resulting in increased viable cell number compared to PW06 treated only. Furthermore, PW06 showed a potent binding ability with hydrophobic interactions in the core site of the Fas-Fas death domains (FADD). In conclusion, PW06 can potent binding ability to the Fas-FADD which led to antiproliferative, cytotoxic activities, and apoptosis induction accompanied by the caspase-dependent and mitochondria-dependent pathways in human pancreatic cancer MIA PaCa-2 cells.

show abstract

Section: Methodsmentioning

confidence: 99%

PW06 Triggered Fas-FADD to Induce Apoptotic Cell Death In Human Pancreatic Carcinoma MIA PaCa-2 Cells through the Activation of the Caspase-Mediated Pathway

Huang

Hsia

Yeh

et al. 2023

Oxidative Medicine and Cellular Longevity

Self Cite

View full text Add to dashboard Cite

show abstract

“…MMP-2, MMP-9 and CyclinD1 (119). In addition, casticin has been shown to inhibit the proliferation of BLCA by inducing DNA damage via decreasing the expression of p-p53 and P-AKT (120). The role of ROS in cell damage and activation of apoptosis is well-established.…”

Section: Flavonoids On Blcamentioning

confidence: 99%

Properties of flavonoids in the treatment of bladder cancer (Review)

Liu

Jia

et al. 2022

Exp Ther Med

View full text Add to dashboard Cite

Given its high recurrence and rapid progress, bladder cancer (BLCA) treatment has become a major problem for clinicians. BLCA is difficult to control even with surgical resection and extensive use of chemotherapeutic drugs. The non-toxicity and ease of accessibility of natural compounds have attracted much attention in recent years. Flavonoids serve an essential role given their antioxidant, antibacterial, anticancer and cardiovascular properties. They are mainly divided into several subclasses; flavones, flavanones, flavonols, flavanols, anthocyanins isoflavones and chalcones. Over the years, the role of flavonoids in BLCA has been extensively studied. The present review provided a comprehensive overview of the classification of flavonoids and substantiate the role of epithelial-mesenchymal transition, cancer stem cells, angiogenesis, epigenetic regulation and programmed cell death in BLCA. The present review emphasized that flavonoids for BLCA treatment are worthy of further study and anti-BLCA drugs have huge prospects for clinical use. Contents 1. Introduction 2. Mechanism 3. Flavonoids on BLCA 4. Discussion and outlook 5. Conclusion

show abstract

“…3-Hydroxyflavone contains a 3-hydroxyl group and has an excellent resonance structure and chelating ability, as well as anti-inflammatory, antitumor, and antiviral properties (23). Casticin, a 3′-hydroxyflavone isolated from Vitex rotundifolia, induces DNA damage and inhibits TSGH-8301 bladder cancer cell viability (24). Additionally, casticin decreases ataxia telangiectasia mutated (ATM) andataxia telangiectasia and Rad3 related (ATR) phosphorylation and downregulates mediator of DNA damage checkpoint 1 (MDC1) and O-6methylguanine-DNA methyltransferase (MGMT).…”

Section: ′-Hydroxyflavonementioning

confidence: 99%

“…Additionally, casticin decreases ataxia telangiectasia mutated (ATM) andataxia telangiectasia and Rad3 related (ATR) phosphorylation and downregulates mediator of DNA damage checkpoint 1 (MDC1) and O-6methylguanine-DNA methyltransferase (MGMT). Moreover, casticin increases p-p53, H2A.X variant histone, and poly-ADP ribose polymerase (PARP) levels, and affects phospho-p53 translocation from the cytoplasm to the nucleus in bladder cancer cells (24). This compound also increases intracellular ROS production, activates the caspase cascade, and disrupts the mitochondrial membrane potential (DYm).…”

Section: ′-Hydroxyflavonementioning

confidence: 99%

Natural Phytochemicals in Bladder Cancer Prevention and Therapy

Xia

Chen

et al. 2021

Front. Oncol.

View full text Add to dashboard Cite

Phytochemicals are natural small-molecule compounds derived from plants that have attracted attention for their anticancer activities. Some phytochemicals have been developed as first-line anticancer drugs, such as paclitaxel and vincristine. In addition, several phytochemicals show good tumor suppression functions in various cancer types. Bladder cancer is a malignant tumor of the urinary system. To date, few specific phytochemicals have been used for bladder cancer therapy, although many have been studied in bladder cancer cells and mouse models. Therefore, it is important to collate and summarize the available information on the role of phytochemicals in the prevention and treatment of bladder cancer. In this review, we summarize the effects of several phytochemicals including flavonoids, steroids, nitrogen compounds, and aromatic substances with anticancer properties and classify the mechanism of action of phytochemicals in bladder cancer. This review will contribute to facilitating the development of new anticancer drugs and strategies for the treatment of bladder cancer using phytochemicals.

show abstract

Casticin Induces DNA Damage and Impairs DNA Repair in Human Bladder Cancer TSGH-8301 Cells

Cited by 17 publications

References 0 publications

PW06 Triggered Fas-FADD to Induce Apoptotic Cell Death In Human Pancreatic Carcinoma MIA PaCa-2 Cells through the Activation of the Caspase-Mediated Pathway

PW06 Triggered Fas-FADD to Induce Apoptotic Cell Death In Human Pancreatic Carcinoma MIA PaCa-2 Cells through the Activation of the Caspase-Mediated Pathway

Properties of flavonoids in the treatment of bladder cancer (Review)

Natural Phytochemicals in Bladder Cancer Prevention and Therapy

Contact Info

Product

Resources

About