Catabolic Half-Lives and Antigenic Relationships of Native, Altered and Commercially Prepared Human Albumins in Rabbits

Abstract

As determined in rabbits, the in vivo plasma survival, i.e. half-life (t½), of albumin produced by the cold-ethanol procedure did not differ significantly from that of native albumin (p>0.5). Plasma survivals of reprocessed and heat-ethanol produced albumins were significantly less than that of the native protein (0.02 < p < 0.05). Chemicallymodified albumin had a considerably reduced t½ (p< 0.001) and was the only albumin shown to be antigenically different from the native protein. Variations in t½s of albumin preparations could not be attributed to differences in their polymer contents.

Therapeutic Solutions of Human Albumin – The Possible Effect of Process-Induced Molecular Alterations on Clinical Efficacy and Safety

“…In pharmacokinetic studies performed in rabbits with different albumin preparations, variations in the half-life of the products were found. These differences were not attributable to the presence of neo-antigens to albumin, Such neo-antigens were found only in a chemically-modified product (161). Preparations subjected to repeated heat treatment as part of the manufacturing process showed the shortest albumin half-life.…”