1995
DOI: 10.1128/jb.177.5.1129-1136.1995
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Catabolic ornithine transcarbamylase of Halobacterium halobium (salinarium): purification, characterization, sequence determination, and evolution

Abstract: Halobacterium halobium (salinarium) is able to grow fermentatively via the arginine deiminase pathway, which is mediated by three enzymes and one membrane-bound arginine-ornithine antiporter. One of the enzymes, catabolic ornithine transcarbamylase (cOTCase), was purified from fermentatively grown cultures by gel filtration and ammonium sulfate-mediated hydrophobic chromatography. It consists of a single type of subunit with an apparent molecular mass of 41 kDa. As is common for proteins of halophilic Archaea,… Show more

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Cited by 33 publications
(27 citation statements)
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“…However, this does not mean that the arcD gene is absent in this bacterium. The organization of the arc genes in an arcDABC operon is not universal: in H. salinarium the arcC gene is located upstream of the gene encoding the OTCase (arcB) and not downstream (46). Another possibility is that the ornithine, formed during the arginine deiminase pathway, is further metabolized.…”
Section: Discussionmentioning
confidence: 99%
“…However, this does not mean that the arcD gene is absent in this bacterium. The organization of the arc genes in an arcDABC operon is not universal: in H. salinarium the arcC gene is located upstream of the gene encoding the OTCase (arcB) and not downstream (46). Another possibility is that the ornithine, formed during the arginine deiminase pathway, is further metabolized.…”
Section: Discussionmentioning
confidence: 99%
“…The genes are organized in an operon, and a polycistronic mRNA is transcribed from a single promoter and subsequently processed into a variety of smaller transcripts (17) in an RNase E-dependent reaction (18). The transcription of the arcDABC operon is under the positive control of the ANR protein (15, 22), a member of the FNR protein family.Within the domain Archaea, arginine-dependent anaerobic growth has only been reported for some halobacterial species (25,37). The consumption of arginine was coupled to the equimolar production of ornithine (25), and therefore it was concluded that the ADI pathway was used.…”
mentioning
confidence: 99%
“…Halobacterial proteins are adapted to high salt concentrations ; typically, they are not stable below an osmolarity of about 1-2 M (e.g., Ruepp et al, 1995), and are produced in E. coli in an inactive form (e.g., Blecher et al, 1993;Cendrin et al, 1993). After isolation of a denatured halobacterial protein it is therefore necessary to find conditions that allow it to properly refold.…”
Section: Resultsmentioning
confidence: 99%
“…The similarity of the deduced polypeptide sequence to the expected regions of eucaryal TBP sequences revealed that indeed part of the halobacterial tbp gene had been amplified. The PCR fragment was used to construct a digoxigenin-labeled, single-stranded probe as described (Ruepp et al, 1995). Southern blot experiments revealed that the probe hybridized to a genomic BamHI fragment of about 4.5 kbp.…”
Section: Microorganismsmentioning
confidence: 99%
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