Catabolism of strigolactones by a carboxylesterase

Xu, Enjun; Chai, Linjiang; Zhang, Shiqi; Yu, Ruixue; Zhang, Xixi; Xu, Chongyi; Hu, Yuxin

doi:10.1038/s41477-021-01011-y

Cited by 33 publications

(25 citation statements)

References 81 publications

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“…Villaécija-Aguilar et al 58 reported that root hair development in d14 is still responsive to 5DS 58 , suggesting a D14 -independent strigolactone receptor. D14L paralogs in pea are able to bind 5DS in vitro and hydrolyse strigolactone-like molecules 59 , raising the possibility that strigolactones or strigolactone-derived molecules 60 may bind and activate D14L.…”

Section: Discussionmentioning

confidence: 99%

Nutrient regulation of lipochitooligosaccharide recognition in plants via NSP1 and NSP2

Sun

Albinsky

et al. 2022

Nat Commun

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Many plants associate with arbuscular mycorrhizal fungi for nutrient acquisition, while legumes also associate with nitrogen-fixing rhizobial bacteria. Both associations rely on symbiosis signaling and here we show that cereals can perceive lipochitooligosaccharides (LCOs) for activation of symbiosis signaling, surprisingly including Nod factors produced by nitrogen-fixing bacteria. However, legumes show stringent perception of specifically decorated LCOs, that is absent in cereals. LCO perception in plants is activated by nutrient starvation, through transcriptional regulation of Nodulation Signaling Pathway (NSP)1 and NSP2. These transcription factors induce expression of an LCO receptor and act through the control of strigolactone biosynthesis and the karrikin-like receptor DWARF14-LIKE. We conclude that LCO production and perception is coordinately regulated by nutrient starvation to promote engagement with mycorrhizal fungi. Our work has implications for the use of both mycorrhizal and rhizobial associations for sustainable productivity in cereals.

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Section: Discussionmentioning

confidence: 99%

Nutrient regulation of lipochitooligosaccharide recognition in plants via NSP1 and NSP2

Sun

Albinsky

et al. 2022

Nat Commun

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“…Very recently, a degradation pathway for SLs has been discovered in Arabidopsis thaliana (Xu et al, 2021 ). It involves a carboxylesterase (AtCXE15), with no SL reception function, which was demonstrated to be able to break SL molecules and thereby modulate shoot branching.…”

Section: Discussionmentioning

confidence: 99%

Expansion of the Strigolactone Profluorescent Probes Repertory: The Right Probe for the Right Application

et al. 2022

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Strigolactones (SLs) are intriguing phytohormones that not only regulate plant development and architecture but also interact with other organisms in the rhizosphere as root parasitic plants (Striga, Orobanche, and Phelipanche) and arbuscular mycorrhizal fungi. Starting with a pioneering work in 2003 for the isolation and identification of the SL receptor in parasitic weeds, fluorescence labeling of analogs has proven a major strategy to gain knowledge in SL perception and signaling. Here, we present novel chemical tools for understanding the SL perception based on the enzymatic properties of SL receptors. We designed different profluorescent SL Guillaume Clavé (GC) probes and performed structure-activity relationship studies on pea, Arabidopsis thaliana, and Physcomitrium (formerly Physcomitrella) patens. The binding of the GC probes to PsD14/RMS3, AtD14, and OsD14 proteins was tested. We demonstrated that coumarin-based profluorescent probes were highly bioactive and well-adapted to dissect the enzymatic properties of SL receptors in pea and a resorufin profluorescent probe in moss, contrary to the commercially available fluorescein profluorescent probe, Yoshimulactone Green (YLG). These probes offer novel opportunities for the studies of SL in various plants.

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“…On account of low abundance of SLs, little is known about their inactivation at the catabolic level ( Snowden et al., 2005 ; Arite et al., 2007 ; Simons et al., 2007 ). In Arabidopsis , AtCXE15 has been identified as a functionally important SLs hydrolase ( Xu et al., 2021 ), and the ectopic expression of CXE20 effectively reduces the concentration of free SLs and increases the number of branches ( Roesler et al., 2021 ). These studies indicate a new mechanism of SLs degradation regulation in plants.…”

Section: Discussionmentioning

confidence: 99%

“…CXEs participate in the activation of plant hormone-signaling substances, regulating IAA metabolism in immature maize endosperm tissues ( Kowalczyk et al., 2003 ). CXE genes also regulate strigolactones (SLs) metabolism in A. thaliana ( Xu et al., 2021 ) and are involved in herbicide activation. For example, in A. thaliana , AtCXE12 shares the properties of the hydrolytic herbicide precursor methyl-2, 4-dichlorophenoxyacetic acid ( Gershater et al., 2007a ).…”

Section: Introductionmentioning

confidence: 99%

“…Another SLs receptor, D14 , is a member of the hydrolase family with α/β-folding characteristics, but its binding effect is greater than that of hydrolysis and may not be the key mechanism in SLs hydrolysis ( Hamiaux et al., 2012 ; Zhao et al., 2013 ). In A. thaliana , AtCXE15 and its homologues have been identified as highly hydrolytic enzymes for SLs; AtCXE15 catalyzes the hydrolysis of various SLs analogs, and overexpression of AtCXE15 induces bud branching by SLs ( Xu et al., 2021 ). Ectopic expression of AtCXE20 in A. thaliana and maize also results in increased plant branching and tillering ( Roesler et al., 2021 ).…”

Section: Introductionmentioning

confidence: 99%

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Comprehensive analysis of the carboxylesterase gene reveals that NtCXE22 regulates axillary bud growth through strigolactone metabolism in tobacco

Wang

Xie²,

Xu³

et al. 2022

Front. Plant Sci.

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Carboxylesterases (CXE) are a class of hydrolytic enzymes with α/β-folding domains that play a vital role in plant growth, development, stress response, and activation of herbicide-active substances. In this study, 49 Nicotiana tabacum L. CXE genes (NtCXEs) were identified using a sequence homology search. The basic characteristics, phylogenetic evolution, gene structure, subcellular location, promoter cis-elements, and gene expression patterns of the CXE family were systematically analyzed. RNA-seq data and quantitative real-time PCR showed that the expression level of CXEs was associated with various stressors and hormones; gene expression levels were significantly different among the eight tissues examined and at different developmental periods. As a new class of hormones, strigolactones (SLs) are released from the roots of plants and can control the germination of axillary buds.NtCXE7, NtCXE9, NtCXE22, and NtCXE24 were homologous to Arabidopsis SLs hydrolase AtCXE15, and changes in their expression levels were induced by topping and by GR24 (a synthetic analogue of strigolactone). Further examination revealed that NtCXE22-mutant (ntcxe22) plants generated by CRISPR-Cas9 technology had shorter bud outgrowth with lower SLs content. Validation of NtCXE22 was also performed in NtCCD8-OE plants (with fewer axillary buds) and in ntccd8 mutant plants (with more axillary buds). The results suggest that NtCXE22 may act as an efficient SLs hydrolase and affects axillary bud development, thereby providing a feasible method for manipulating endogenous SLs in crops and ornamental plants.

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Catabolism of strigolactones by a carboxylesterase

Cited by 33 publications

References 81 publications

Nutrient regulation of lipochitooligosaccharide recognition in plants via NSP1 and NSP2

Nutrient regulation of lipochitooligosaccharide recognition in plants via NSP1 and NSP2

Expansion of the Strigolactone Profluorescent Probes Repertory: The Right Probe for the Right Application

Comprehensive analysis of the carboxylesterase gene reveals that NtCXE22 regulates axillary bud growth through strigolactone metabolism in tobacco

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