Catabolite Inactivation of the Glucose Transport System in Saccharomyces cerevisiae

Busturia, Ana; Lagunas, Rosario

doi:10.1099/00221287-132-2-379

Cited by 104 publications

(123 citation statements)

References 16 publications

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“…This is, for the first time, a genetical proof that the yeast hexose-uptake system also mediates uptake of xylose. It confirms earlier results obtained by kinetic and physiological analysis of xylose uptake in yeast cells (Serrano & de la Fuente, 1974 ;Busturia & Lagunas, 1986 ;Ko$ tter & Ciriacy, 1993 ;Meinander & Hahn-Ha$ gerdal, 1997). After overexpression of individual hexose-transporter genes in the xylose uptake-deficient yeast strain, we could demonstrate that at xylose concentrations of 2 % only Hxt4, Hxt5, Hxt7 and Gal2 are able to transport the sugar in significant amounts.…”

Section: Discussionsupporting

confidence: 80%

Characterization of the xylose-transporting properties of yeast hexose transporters and their influence on xylose utilization

et al. 2002

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For an economically feasible production of ethanol from plant biomass by microbial cells, the fermentation of xylose is important. As xylose uptake might be a limiting step for xylose fermentation by recombinant xyloseutilizing Saccharomyces cerevisiae cells a study of xylose uptake was performed. After deletion of all of the 18 hexose-transporter genes, the ability of the cells to take up and to grow on xylose was lost. Reintroduction of individual hexose-transporter genes in this strain revealed that at intermediate xylose concentrations the yeast high-and intermediate-affinity transporters Hxt4, Hxt5, Hxt7 and Gal2 are important xylose-transporting proteins. Several heterologous monosaccharide transporters from bacteria and plant cells did not confer sufficient uptake activity to restore growth on xylose. Overexpression of the xylose-transporting proteins in a xylose-utilizing PUA yeast strain did not result in faster growth on xylose under aerobic conditions nor did it enhance the xylose fermentation rate under anaerobic conditions. The results of this study suggest that xylose uptake does not determine the xylose flux under the conditions and in the yeast strains investigated.

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Section: Discussionsupporting

confidence: 80%

Characterization of the xylose-transporting properties of yeast hexose transporters and their influence on xylose utilization

et al. 2002

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“…With respect to sugars, it is known that maltose [4] as well as glucose transport systems [5] consist of two components that, in the latter case, are controlled differently by catabolite repression [6] and by the presence of hexose kinases [7]. To our knowledge, the existence of several forms in the case of the galactose transport system has not yet been explored.…”

Section: Introductionmentioning

confidence: 99%

“…Transport systems in Saccharomyces cerevisiae are very often composed of several forms distinguishable by their different affinity constants for the respective substrates [1][2][3][4][5][6][7]. With respect to sugars, it is known that maltose [4] as well as glucose transport systems [5] consist of two components that, in the latter case, are controlled differently by catabolite repression [6] and by the presence of hexose kinases [7].…”

Section: Introductionmentioning

confidence: 99%

Inactivation of the galactose transport system in Saccharomyces cerevisiae

DeJuan

Lagunas

1986

FEBS Letters

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The galactose transport system of Saccharomyces cerevisiae consists of one component which shows a K m value of approx. 4 mM in growing cells. A rapid and irreversible inactivation of this transport is detected on impairment of protein synthesis. This inactivation shows the following characteristics: (i) it is due to changes in the K m and V max of the transport system; (ii) it follows first‐order kinetics; (iii) it is an energy‐dependent process and is stimulated by the presence of an exogenous carbon source; (iv) fermentable substrates stimulate inactivation more efficiently than non‐fermentable substrates.

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“…Without a usable nitrogen source (FAN), no protein synthesis and biomass synthesis can occur. Additionally, due to an inactivation of glucose transport systems less glucose can be taken in by the yeast cells 5 . The limitation effect is formulated by an additional Monod term in the expression for the substrate uptake rate, with a half saturation constant K n = 2 mmol/L 6 .…”

Section: )79087 1exliqexmgep Qshippmrkmentioning

confidence: 99%

“…A lack of nitrogen and trace elements, e.g. zinc, causes limitation effects 5,22 . Besides, ethanol production during the growth process causes additional inhibition effects 1 .…”

Section: -2863(9'8-32mentioning

confidence: 99%

A Model Based Simulation of Brewing Yeast Propagation

Kurz

Mieleitner

Becker

et al. 2002

Journal of the Institute of Brewing

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A comprehensive kinetic model of yeast propagation in breweries is introduced. It represents the basis for a control strategy aimed at the provision of optimal inoculum at the starting time of subsequent industrial beer fermentations. In the metabolic modelling approach presented, respiratory metabolism on sugars and ethanol and fermentative metabolism respectively, are included. Occurring limitations, due to specific nutritional data of the growth medium, were taken into account for sugar, nitrogen, ethanol and oxygen. Correspondingly, inhibitions of the metabolism by ethanol and high sugar concentrations were formulated. The model especially represents the Crabtree-effect. For model validation, literature data were used and selected experiments within the relevant range of manipulated variables (temperature, dissolved oxygen) were conducted. Model based simulations matched these data with a deviation of 5.6% and with standard deviations of 5.6% after an adaptation of three variable parameters. A relationship between the temperature and the variable parameters could be extracted, which allowed predictive simulations of the yeast propagation process using non-isothermal trajectories. It was shown, that with a precise adjustment of trajectories of both, temperature and dissolved oxygen, the crop time of the inoculum could be varied within a period of 7 to 50 h to maintain high fermentation activity for the subsequent anaerobic fermentation.

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Catabolite Inactivation of the Glucose Transport System in Saccharomyces cerevisiae

Cited by 104 publications

References 16 publications

Characterization of the xylose-transporting properties of yeast hexose transporters and their influence on xylose utilization

Characterization of the xylose-transporting properties of yeast hexose transporters and their influence on xylose utilization

Inactivation of the galactose transport system in Saccharomyces cerevisiae

A Model Based Simulation of Brewing Yeast Propagation

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