Catalase Activity and Post-anoxic Injury in Monocotyledonous Species

Monk, Lorna S.; Braendle, Roland; Crawford, R. M. M.

doi:10.1093/jxb/38.2.233

Cited by 78 publications

(58 citation statements)

References 20 publications

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“…High post-anoxic levels of AA have been observed in potato tubers 5 h after re-aeration, following anoxic treatment for 24 to 72 h (Pfister-Sieber and Braendle, 1994). A 5-fold increase in AA has been observed 30 min after transfer to air for anoxic Glyceria maxima rhizomes (Monk et al, 1987a). The conversion of anaerobically accumulated ethanol to AA may account for these observations and may be one of the causes of post-anoxic injury (Studer and Braendle, 1988).…”

Section: Post-anoxic Aa Emissionmentioning

confidence: 99%

“…Two main pathways of ethanol oxidation, i.e. NAD+-dependent conversion of ethanol to AA catalyzed by the action of ADH, have been proposed (Monk et al, 1987a): (1) and H,O,-dependent catalase-controlled peroxidation…”

Section: Post-anoxic Aa Emissionmentioning

confidence: 99%

“…This reaction requires H,O,, and the most probable source is dismutation of the superoxide radical (O,-) within mitochondria by superoxide dismutase (Monk et al, 198%;Scandalios, 1994). The high level of reducing equivalents (NADH) within mitochondria prevailing in the anoxic tissue just before re-exposure to O, (Reggiani at al., 1985;Van Toai and Bolles, 1991) may well favor a rapid generation of active O, species, e.g.…”

Section: Post-anoxic Aa Emissionmentioning

confidence: 99%

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Dynamics of Acetaldehyde Production during Anoxia and Post-Anoxia in Red Bell Pepper Studied by Photoacoustic Techniques

et al. 1997

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Acetaldehyde (AA), ethanol, and CO, production in red bell pepper (Capsicum annum 1.) fruit has been measured in a continu o u~ flow system as the fruit was switched between 20% O, and anaerobic conditions. Minimum gas phase concentrations of 0.5 nL L-l, 10 nL 1-', and 1 mL L-', respectively, can be detected employing a laser-based photoacoustic technique. This technique allows monitoring of low production rates and transient features in real time. At the start of anaerobic treatment respiration decreases by 60% within 0.5 h, whereas AA and ethanol production is delayed by 1 to 3 h. This suggests a direct slow-down of the tricarboxylic acid cycle and a delayed onset of alcoholic fermentation. Reexposure of the fruit to oxygen results in a 2-to 10-fold upsurge in AA production. A short anoxic period leads to a sharp transient peak lasting about 40 min, whereas after numerous and longer anoxic periods, post-anoxic AA production stays high for severa1 hours. High sensitivity of the fruit tissue to oxygen is further evidenced by a sharp decrease in post-anoxic AA production upon an early return to anaerobic conditions. Ethanol oxidatioo by the "peroxidatic" action of catalase is proposed to account for the immediate postanoxic AA upsurge.Fermentation occurs in fruits when oxygen flux to respiring cells is reduced below a critica1 value. Hypoxia occurs in bulky fruits under natural conditions during normal ripening due to impaired gas exchange with the atmosphere. The metabolic response and adaptation of plants to anaerobiosis has been extensively reviewed (Perata and Alpi, 1993; Pfister-Sieber and Braendle, 1994). Low oxygen concentrations are widely used in controlled atmosphere storage of harvested fruit, e.g. apples, with the goal of prolonging fruit shelf-life (Knee, 1991). Reduction of respiration rate and ethylene biosynthesis during controlled atmosphere storage does not, however, induce fermentation. Recently, storage in 1% O, was shown to extend the postharvest life of bell peppers (Lu0 and Mikitzel, 1996). Similarly, short-term anoxic treatments of harvested fruit improve fruit aroma and quality (Pesis, 1995). The physiological basis of the observed effects has yet to be clarified.

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Section: Post-anoxic Aa Emissionmentioning

confidence: 99%

Section: Post-anoxic Aa Emissionmentioning

confidence: 99%

Section: Post-anoxic Aa Emissionmentioning

confidence: 99%

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Dynamics of Acetaldehyde Production during Anoxia and Post-Anoxia in Red Bell Pepper Studied by Photoacoustic Techniques

et al. 1997

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“…Monk et al ( 14) suggested that the reaction of acetaldehyde with proteins to give cross-linkages, carbinolamines, and Schifis bases may be responsible for cell death during the postanoxic phase. Acetaldehyde binds to proteins leading to the formation of both stable and unstable adducts (8); the latter, postulated to be Schiffs bases, can be stabilized in vitro ' Research supported by National Research Council of Italy, Special Project RAISA, Subproject 2,paper No.…”

mentioning

confidence: 99%

Immunological Detection of Acetaldehyde-Protein Adducts in Ethanol-Treated Carrot Cells

Perata¹,

Vernieri²,

Armellini³

et al. 1992

Plant Physiol.

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Polyclonal antibodies able to recognize protein-acetaldehyde conjugates were produced and characterized. The antibodies react with sodium cyanoborohydride-reduced Schiff's bases between acetaldehyde and a protein, independently of the nature of the macromolecule binding the acetaldehyde moiety. Only conjugates between acetaldehyde or propionaldehyde and a protein are recognized; conjugates obtained with other aldehydes are not reactive. Results conceming the formation of acetaldehyde adducts with carrot (Daucus carota L.) proteins are presented as well as the presence of such conjugates in ethanoltreated carrot cell cultures, a system highly sensitive to the presence of ethanol in the culture medium.

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“…concentration (5,19) have been shown to influence catalase activity in mature plants. For example, Fair et al (5) demonstrated that catalase activity was suppressed when barley was grown in 1 to 5% CO2 and in 2% 02.…”

mentioning

confidence: 99%

Regulation of Catalase Activity in Leaves of Nicotiana sylvestris by High CO₂

Havir¹,

McHale²

1989

Plant Physiol.

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The effect of high CO2 (1% C02/21% 02) on the activity of specific forms of catalase (CAT-1, -2, and -3) (EA Havir, NA McHale [1987] Plant Physiol 84: 450-455) in seedling leaves of tobacco (Nicotiana sylvestris, Nicotiana tabacum) was examined. In high C02, total catalase activity decreased by 50% in the first 2 days, followed by a more gradual decline in the next 4 days. The loss of total activity resulted primarily from a decrease in CAT-1 catalase. In contrast, the activity of CAT-3 catalase, a form with enhanced peroxidatic activity, increased 3-fold in high CO2 relative to aiontrols after 4 days. Short-term exposure to high CO2 indicated that the 50% loss of total activity occurs in the first 12 hours. Catalase levels increased to normal within 12 hours after seedlings were retumed to air. When seedlings were transferred to air after prolonged exposure to high CO2 (13 days), the levels of CAT-1 catalase were partially restored while CAT-3 remained at its elevated level. Levels of superoxide dismutase activity and those of several peroxisomal enzymes were not affected by high CO2. Total catalase levels did not decline when seedlings were exposed to atmospheres of 0.04% C02/5% 02 or 0.04% C02/1% 02, indicating that regulation of catalase in high CO2 is not related directly to suppression of photorespiration. Antibodies prepared against CAT-1 catalase from N. tabacum reacted strongly against CAT-1 catalase from both N. sylvestris and N. tabacum but not against CAT-3 catalase from either species. This observation, along with the rapid changes in CAT-I and the much slower changes in CAT-3 suggest that one form is not directly derived from the other. concentration (5, 19) have been shown to influence catalase activity in mature plants. For example, Fair et al. (5) demonstrated that catalase activity was suppressed when barley was grown in 1 to 5% CO2 and in 2% 02. Other peroxisomal enzymes were suppressed also and thus the effect in barley may be generally on peroxisomal turnover. In cases where catalase regulation is independent of peroxisomal turnover (e.g. Refs. 4, 6, and this paper) the mechanism of regulation has not been determined but could be the result of changes in rates of synthesis, degradation, or by covalent modification of the protein. The determination by Eising et al. (3) that the sharp decline in catalase activity in greening sunflower cotyledons was due to the formation of an inactive but largely intact protein molecule may be pertinent to studies of environmentally related changes in catalase activity.We previously showed that catalase activity in leaves of tobacco seedlings was distributed among several forms, one ofwhich had enhanced peroxidatic activity and that there was a change in distribution with time of development (l1). Furthermore, transfer of the seedlings to 1% C02/2 1% 02 for 4 d reduced total catalase activity by about 50% and altered the distribution ofthe activities. Here we show that the decline in catalase in high CO2 is very rapid and results primarily from the los...

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Catalase Activity and Post-anoxic Injury in Monocotyledonous Species

Cited by 78 publications

References 20 publications

Dynamics of Acetaldehyde Production during Anoxia and Post-Anoxia in Red Bell Pepper Studied by Photoacoustic Techniques

Dynamics of Acetaldehyde Production during Anoxia and Post-Anoxia in Red Bell Pepper Studied by Photoacoustic Techniques

Immunological Detection of Acetaldehyde-Protein Adducts in Ethanol-Treated Carrot Cells

Regulation of Catalase Activity in Leaves of Nicotiana sylvestris by High CO₂

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Catalase Activity and Post-anoxic Injury in Monocotyledonous Species

Cited by 78 publications

References 20 publications

Dynamics of Acetaldehyde Production during Anoxia and Post-Anoxia in Red Bell Pepper Studied by Photoacoustic Techniques

Dynamics of Acetaldehyde Production during Anoxia and Post-Anoxia in Red Bell Pepper Studied by Photoacoustic Techniques

Immunological Detection of Acetaldehyde-Protein Adducts in Ethanol-Treated Carrot Cells

Regulation of Catalase Activity in Leaves of Nicotiana sylvestris by High CO2

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Regulation of Catalase Activity in Leaves of Nicotiana sylvestris by High CO₂