Catalase and chondroitin sulfate derivatives against thrombotic effect induced by reactive oxygen species in a rat artery

Maksimenko, A. V.; Golubykh, V. L.; Tischenko, Elena G.

doi:10.1016/s1096-7176(03)00026-0

Cited by 18 publications

(22 citation statements)

References 24 publications

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“…This view is confirmed by the data of adenovirus-mediated extracellular SOD gene therapy in respect to the decrease of the neointima formation in balloon-denuded rabbit aorta [55]. Moreover, the effect of gene therapy was pronounced only after local (via catheter), but not systemic (intraaortally without catheter) gene delivery [55], whereas the modified antioxidant enzymes were active therapeutically into rat artery after systemic (intravenous bolus) administration [13,14]. On the other hand, destruction of the glycocalyx by active oxygen metabolites promotes cellular injury [56,57].…”

Section: Antithrombotic Activity Of Sod and Chs Derivativesmentioning

confidence: 60%

“…(2C), and, respectively, between native CAT and the control (not shown), on the other hand. The antithrombotic effects of CAT-CHS and CAT+CHS are similar and significantly exceed the effect of native CAT and free CHS [13]. In a rat model of arterial injury, the effect of CAT is associated with inhibition of platelet aggregation.…”

Section: Antithrombotic Activities Of Cat and Chs Derivativesmentioning

confidence: 92%

“…(2) implies contribution of both components of these agents [13]. After bolus administration of CAT preparations at the given doses (by active enzyme) the arterial blood flow never reached the zero level during an hour monitoring Fig.…”

Section: Antithrombotic Activities Of Cat and Chs Derivativesmentioning

confidence: 99%

“…Obviously, exogenous CHS could reduce the probability of platelet activation due to membrane-bound CHS. So far, we have studied their effects only in vivo [13,14]. Special studies are necessary to elucidate the mechanisms responsible for the effects of CAT and CHS preparations on platelet aggregation.…”

Section: Antithrombotic Activities Of Cat and Chs Derivativesmentioning

confidence: 99%

“…A comparative study of thrombus formation in a ferrous chloride and a ferric chloride rat model has revealed a more severe thrombosis in the former model [8]. Thus, we have chosen the ferrous chloride rat model of arterial injury to examine the protective effects of superoxide dismutase *Address correspondence to this author at the Institute of Experimental Cardiology, Russian Cardiology Research Centre, 3 rd Cherepkovskaya str., Moscow 121552, Russia; Fax: +7-95-415-29-62, E-mail: alexmak@cardio.ru and catalase and of their covalent and non-covalent complexes [13,14].…”

Section: Introductionmentioning

confidence: 99%

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Experimental Antioxidant Biotherapy for Protection of the Vascular Wall by Modified Forms of Superoxide Dismutase and Catalase

Maksimenko¹

2005

CPD

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The antithrombotic activities of superoxide dismutase and catalase are determined by their effects on reactive oxygen species. Modification of these enzymes with chondroitin sulphate enhances the effect due to accumulation of the derivatives on the surface of the vascular wall cells. We have shown that the effects of covalently modified biocatalysts exceed those of native enzymes, free chondroitin sulphate and their mixtures. The superoxide dismutase-chondroitin sulphate conjugate markedly reduced the thrombus mass, while the catalase-chondroitin sulphate conjugate predominantly preserved blood flow. The magnitude and duration of the antithrombotic activity of modified enzymes in a rat arterial thrombosis model allows one to expect a considerable protective effect after their combined application. A single-bolus intravenous injection of the combination between superoxide dismutase-chondroitin sulphate and catalase-chondroitin sulphate covalent conjugates had a significantly lower antithrombotic effect compared with that of the superoxide dismutase-chondroitin sulphate-catalase bienzymic covalent conjugate. This could be explained by different surface distribution of the conjugates in the circulation after their intravenous administration. Biomedical study of this approach promises a new therapeutic strategy of simple and effective protection of the vascular wall against various injuries with the use of the covalent conjugate superoxide dismutase-chondroitin sulphate-catalase. The review analyses the trends of combined application of enzyme preparations to enhance the effect of antioxidant therapy and to develop conjunctive courses of thrombolytic treatment.

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