Catalase and superoxide dismutase response and the underlying molecular mechanism for naphthalene

Jing, Mingyang; Han, Guangye; Wan, Jingqiang; Zhang, Shansheng; Yang, Junzhong; Zong, Wansong; Niu, Qigui; Liu, Rutao

doi:10.1016/j.scitotenv.2020.139567

Cited by 88 publications

(20 citation statements)

References 39 publications

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“…The proposition of an action mechanism explaining the increase in these enzymes’ activity is very incipient, either due to our study’s pioneering nature or the need to deepen biochemical assessments in future studies. However, it is tempting to speculate that the interactions between PSPD2002 and PSPD2003 peptides and antioxidant enzymes evaluated in tadpoles (confirmed by molecular docking) have induced functional changes in SOD and catalase, similarly to what was observed by Jing et al (2020) by exposing hepatocytes isolated from C57BL6 mice to different concentrations of naphthalene. The data obtained from the molecular docking reinforces our hypothesis by confirming the affinity between the PSPD2002 and PSPD2003 peptides and the referred enzymes and the existence of interactions with residues from all tested moorings (Figure 6).…”

Section: Resultsmentioning

confidence: 61%

“…The activation or suppression of antioxidant activity by peptides was assessed by determining catalase and superoxide dismutase (SOD) enzyme activities, which are considered critical first-line antioxidants for defense strategies against oxidative stress (Ighodaro&Akinloye, 2018; Jing et al, 2020). While catalase activity was assessed based on Sinha et al (1972) andMontalvão et al (2021).…”

Section: Methodsmentioning

confidence: 99%

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An insight into neurotoxic and toxicity of spike fragments SARS-CoV-2 by exposure environment: A threat to aquatic health?

Charlie-Silva

Araújo

Guimarães

et al. 2021

Preprint

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The Spike protein (S protein) is a critical component in the infection of the new coronavirus (SARS-CoV-2). The objective of this work was to evaluate whether peptides from S protein could cause negative impact in the aquatic animals. The aquatic toxicity of SARS-CoV-2 spike protein peptides derivatives has been evaluated in tadpoles (n = 50 tadpoles / 5 replicates of 10 animals) from species Physalaemus cuvieri (Leptodactylidae). After synthesis, purification, and characterization of peptides (PSDP2001, PSDP2002, PSDP2003) an aquatic contamination has been simulatedwith these peptides during 24 hours of exposure in two concentrations (100 and 500 ng/mL). The control group (“C”) was composed of tadpoles kept in polyethylene containers containing de-chlorinated water. Oxidative stress, antioxidant biomarkers and neurotoxicity activity were assessed. In both concentrations, PSPD2002 and PSPD2003 increased catalase and superoxide dismutase antioxidants enzymes activities, as well as oxidative stress (nitrite levels, hydrogen peroxide and reactive oxygen species). All three peptides also increased acetylcholinesterase activity in the highest concentration. These peptides showed molecular interactions in silico with acetylcholinesterase and antioxidant enzymes. Aquatic particle contamination of SARS-CoV-2 has neurotoxics effects in P. cuvieri tadpoles. These findings indicate that the COVID-19 can constitute environmental impact or biological damage potential.HIGHLIGHTSSARS-CoV-2 spike protein peptides (PSDP) were synthesized, purified, and characterized by solid phase peptide synthesis.PSDP peptides promoted REDOX imbalance and acute neurotoxicity in tadpoles (Physalaemus cuvieri)In silico studies have shown interactionsbetween peptides and acetylcholinesterase and antioxidant enzymesAquatic particle contamination of SARS-CoV-2 can constitute additional environmental damageGRAPHICAL ABSTRACT

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Section: Resultsmentioning

confidence: 61%

Section: Methodsmentioning

confidence: 99%

An insight into neurotoxic and toxicity of spike fragments SARS-CoV-2 by exposure environment: A threat to aquatic health?

Charlie-Silva

Araújo

Guimarães

et al. 2021

Preprint

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“…Increased levels of CAT and POD were found by Karmakar and Padhy [21] as indicator of stress triggered by PM-bound heavy metals. Increased gene expression of POD and CAT was found in tomato seedlings exposed to heavy metal stress [20].…”

Section: Introductionmentioning

confidence: 95%

Effects of urban atmospheric particulate matter on higher plants using Lycopersicon esculentum as model species

2021

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Atmospheric particulate matter (PM) is one of the major environmental concerns in Europe. A wide range of studies has proved the ecotoxic potential of atmospheric particles. PM exerts chemical stress on vegetation by its potentially toxic constituents; however, relatively few studies are available on assessing phytotoxic effects under laboratory conditions. In our study, aqueous extract of particulate matter was prepared and used for treatment. Experiment was following the procedure defined by the No. 227 OECD Guideline for the Testing of Chemicals: Terrestrial Plant Test. Tomato (Lycopersicon esculentum Mill.) plants were used; elucidated toxicity was assessed based on morphological and biochemical endpoints such as biomass, chlorophyll-a and chlorophyll-b, carotenoids, and protein content. Biomass reduction and protein content showed a clear dose–effect relationship; the biomass decreased in comparison with the control (100%) in all test groups (TG) at a steady rate (TG1: 87.73%; TG2: 71.77%; TG3: 67.01%; TG4: 63.63%). The tendency in protein concentrations compared to the control was TG1: 113.61%; TG2: 148.21% TG3: 160.52%; TG4: 157.31%. However, pigments showed a ‘Janus-faced’ effect: nutrient content of the sample caused slight increase at lower doses; actual toxicity became apparent only at higher doses (chlorophyll-a concentration decrease was 84.47% in TG4, chlorophyll-b was 77.17%, and finally, carotene showed 83.60% decrease in TG4).

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“…1. This stress response derived from the accumulation of reactive oxygen species (ROS) will force the body to form an antioxidant defense system (Jing et al 2020). Reducing the production and eliminating the generation of ROS are the two lines of defense for organisms (Czarna &Jarmuszkiewicz 2006).…”

Section: Introductionmentioning

confidence: 99%

Response of earthworm coelomocytes and catalase to pentanone and hexanone: a revelation of the toxicity of conventional solvents at the cellular and molecular level

Sun

et al. 2022

Environ Sci Pollut Res

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Organic solvents like 2-Pentanone and 2-Hexanone which are widely used in industrial production play an important role in the source of chemical pollution. Based on the current gaps in 2-Pentanone and 2-Hexanone cytotoxicity studies, the earthworms in the soil, which are susceptible to solvent leakage and volatilization, were selected as the receptor. The cytotoxicity of 2-Pentanone and 2-Hexanone was revealed by measuring the multiple intracellular indicators of oxidative stress. At the molecular level, changes in the structure and function of antioxidant enzyme catalase (CAT) were characterized in vitro by a variety of spectroscopy methods and molecular docking. The results show that 2-Pentanone and 2-Hexanone that induced the accumulation of intracellular reactive oxygen species can eventually decrease the cell viability of coelomocytes, accompanied by the regular changes of antioxidant activity and lipid peroxidation level. In addition, the exposure of 2-Pentanone and 2-Hexanone can shrink the backbone structure of CAT, quench the uorescence and misfold the secondary structure. The decrease in enzyme activity should be attributed to the structural changes induced by surface binding. This study discussed the toxicological effects and mechanisms of commonly used organic solvents at the cellular and molecular level, which creatively proposed a new combined method.

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Catalase and superoxide dismutase response and the underlying molecular mechanism for naphthalene

Cited by 88 publications

References 39 publications

An insight into neurotoxic and toxicity of spike fragments SARS-CoV-2 by exposure environment: A threat to aquatic health?

An insight into neurotoxic and toxicity of spike fragments SARS-CoV-2 by exposure environment: A threat to aquatic health?

Effects of urban atmospheric particulate matter on higher plants using Lycopersicon esculentum as model species

Response of earthworm coelomocytes and catalase to pentanone and hexanone: a revelation of the toxicity of conventional solvents at the cellular and molecular level

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