Catalase (CAT) and Ascorbate Peroxidase (APX) Genes Expression Level in Growth of Banana Plantlets (Musa acuminata) cv. Ambon Lumut Under Chromium Stress Condition

Amalia, Lida; Taufikurahman,; Widiyanto, Sri Nanan

doi:10.3923/jps.2016.69.74

Cited by 5 publications

(14 citation statements)

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“…In the present study, the results clearly showed the harmful effects of chromium on banana in vitro growth at morphological, physiological, and molecular levels. The results are matched with the previous findings of Amalia et al, (2016). They reported that, the growth rate of banana plantlets decreased by higher concentrations (200 and 400 ppm) of Cr.…”

Section: Discussionsupporting

confidence: 92%

“…There is a lack of research work on the effect of Cr on banana in vitro performance. According to the available literature, there is only one previous study focused on banana in vitro growth under Cr stress (Amalia et al, 2016). Increasing Cr concentration in plant cells caused the production of Reactive Oxygen Species (ROS) that would successively increase oxidative stress.…”

Section: Discussionmentioning

confidence: 99%

“…The phytotoxicity of Cr can be mediated either by direct interaction with different plant parts and metabolic pathways or it generates internal stress by inducing the accumulation of reactive oxygen species (ROS) (Abdul Wakeel et al, 2020). The effect of Cr on banana growth has been evaluated Amalia et al (2016). The higher concentration of Cr in the medium decreased plan weight drastically.…”

Section: Introductionmentioning

confidence: 99%

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Evaluation of Chromium harmful effects on in vitro banana performance

Youssef

Elsherbini

Morsy

2022

AJAS

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Abiotic stresses have a significant negative impact on plant growth and production. Among which, toxicity of heavy metals is considered one of the most factors affecting plant growth and agriculture on new reclaimed lands. In this study, the harmful effect of chromium was evaluated on in vitro banana plants of Grand Nain and Williams-Zeef cultivars based on morphological, physiological, and molecular assessments. Results showed that chromium significantly decreased all studied traits, including plant fresh weight, plant length, number of shoots per explant, and photosynthesis related pigments (chlorophyll and carotenoid content). The percentage of reduction due to chromium treatment ranged from 36.48 to 79.69% in shoot length of Grand Nain and chlorophyll-b in Williams-Zeef, respectively. Both banana cultivars were negatively affected by chromium treatment. However, Williams-Zeef showed higher reduction than that of Grand Nain in all studied traits. On the other hand, molecular analysis was performed to detect any variation between chromium-treated and untreated plants using inter simple sequence repeats (ISSR) and start codon targeted (SCoT) polymorphism. Results of molecular analysis confirmed the morphophysiological findings, by detection some polymorphic bands due to chromium treatment. In this regard, a total of six polymorphic bands were detected in the two banana cultivars, discriminating treated and control plants. In agreement with morphophysiological results, Williams-Zeef showed more polymorphism (five bands) due to chromium treatment than Grand Nain (one band). The screening protocol used in this study was efficient and helpful and could be used in successive studies to evaluate other toxicants and with other plant species as well.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Evaluation of Chromium harmful effects on in vitro banana performance

Youssef

Elsherbini

Morsy

2022

AJAS

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“…Surprisingly, the expression of most genes that correspond to AOS enzymes was decreased both in leaves and roots. Taufikurahman & Widiyanto (2016) studied the overall expression of CAT and APX in control and under different chromium concentration treatments for Musa acuminata. The CAT and APX expression levels in roots increase 9 and 3 times under chromium stress of 200 ppm, respectively.…”

Section: Experimental Data Concerning the Antioxidant System Componentsmentioning

confidence: 99%

“…Aerobic metabolism provides great energetic benefits to organisms but has byproducts such as forms of reactive oxygen species (ROS), including singlet oxygen, superoxide including northern blot analysis/PCR techniques and/or measurements of enzyme activity during stresses, which revealed the ambiguity of the transcription regulation and, in some cases, indirect relationship between changes of mRNA level and enzyme activity during treatment for Nicotiana plumbaginifolia L. (Tsang et al, 1991), Raphanus sativus L. (Lopez et al, 1996), Arabidopsis thaliana L. (Kandlbinder et al, 2004). Recent studies address more precise ways to quantify antioxidant genes using qRT-PCR methods for Musa acuminata L. (Taufikurahman & Widiyanto, 2016) and Solanum tuberosum L. (El-Argawy & Adss, 2016) and also showing advanced techniques of choosing the reference Figure 1 A general scheme of interaction between AOS components including superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPX), ascorbate peroxidase (APX), dehydroascorbate reductase (DHAR), monohydroascorbate reductase (MDHAR), glutathione reductase (GR), NADPH-dependent thioredoxin reductase (NTRC), ascorbate (AsA), monodehydroascorbate (MDHA), dehydroascorbate (DHA), glutathione (GSH), glutathione disulfide (GSSG), reduced (TRX) and oxidized (OX-TRX) forms of thioredoxin, reduced (GRX) and oxidized (OX-GRX) forms of glutaredoxin. Ascorbate-glutathione cycle is indicated as "ASC-GSH cycle".…”

Section: Introductionmentioning

confidence: 99%

Subcellular compartmentalization of the plant antioxidant system: an integrated overview

Bobrovskikh

Зубаирова

Kolodkin

et al. 2020

PeerJ

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The antioxidant system (AOS) maintains the optimal concentration of reactive oxygen species (ROS) in a cell and protects it against oxidative stress. In plants, the AOS consists of seven main classes of antioxidant enzymes, low-molecular antioxidants (e.g., ascorbate, glutathione, and their oxidized forms) and thioredoxin/glutaredoxin systems which can serve as reducing agents for antioxidant enzymes. The number of genes encoding AOS enzymes varies between classes, and same class enzymes encoded by different gene copies may have different subcellular localizations, functional loads and modes of evolution. These facts hereafter reinforce the complex nature of AOS regulation and functioning. Further studies can describe new trends in the behavior and functioning of systems components, and provide new fundamental knowledge about systems regulation. The system is revealed to have a lot of interactions and interplay pathways between its components at the subcellular level (antioxidants, enzymes, ROS level, and hormonal and transcriptional regulation). These facts should be taken into account in further studies during the AOS modeling by describing the main pathways of generating and utilizing ROS, as well as the associated signaling processes and regulation of the system on cellular and organelle levels, which is a complicated and ambitious task. Another objective for studying the phenomenon of the AOS is related to the influence of cell dynamics and circadian rhythms on it. Therefore, the AOS requires an integrated and multi-level approach to study. We focused this review on the existing scientific background and experimental data used for the systems biology research of the plant AOS.

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Establishment of an in vitro Protocol for Chromium Toxicity Tolerance Screening in Banana

Abdelhafiz

Elsherbini

Youssef

2022

Journal of Sohag Agriscience (JSAS)

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Several factors negatively affecting plant growth and production. Abiotic stresses play a vital role against many plant species. Among various abiotic stresses, heavy metals provide one of the most harmful effects on plant performance. Thus, this study was designed to establish an efficient in vitro protocol for chromium (Cr) toxicity tolerance screening in banana. One of the most important commercial banana cultivars (i.e., Grand Nain) was used. Five different concentrations of potassium dichromate (i.e., 50, 100, 200, 400 and 600 ppm) were tested along with free chromium medium as a control. Analysis of variance showed highly significant differences among Cr concentrations, affecting shoot length and number of shoots per explants, compared to control. In addition, results clearly showed the harmful effects of chromium on both shoot length and number of shoots per explant. In this regard, the percentage of reduction in shoot length due to Cr treatment ranged from 5.77 to 84.09% regarding treatment with 50 and 600ppm, respectively. Moreover, the number of shoots per explant was decreased by a range of 6.00 to 86.48% caused by treatment of 50 and 600ppm, respectively. According to these results and the analysis of variance, it is recommended to use the concentration of 400ppm potassium dichromate for in vitro screening of chromium tolerance in banana. The established protocol is efficient and of great importance and could be used for selecting chromium-tolerant genotypes in banana and other species.

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Catalase (CAT) and Ascorbate Peroxidase (APX) Genes Expression Level in Growth of Banana Plantlets (Musa acuminata) cv. Ambon Lumut Under Chromium Stress Condition

Cited by 5 publications

References 8 publications

Evaluation of Chromium harmful effects on in vitro banana performance

Evaluation of Chromium harmful effects on in vitro banana performance

Subcellular compartmentalization of the plant antioxidant system: an integrated overview

Establishment of an in vitro Protocol for Chromium Toxicity Tolerance Screening in Banana

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