Catalase Effects on Glucose-Sensitive Hydrogels

Jung, Dal-Young; Magda, Jules J.; Han, Inn Oc

doi:10.1021/ma992098b

Cited by 50 publications

(40 citation statements)

References 42 publications

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“…Polyelectrolyte hydrogels of a type widely studied in the literature [3,[13][14][15] were prepared from three monomers: hydroxypropyl methacrylate(HPMA), (N,N-dimethylamino)ethyl methacrylate (DMA), and tetraethylene glycol dimethacrylate (TEGDMA). The monomer DMA contains a pH-sensitive tertiary amine, TEGDMA is a crosslinker, and HPMA was included to obtain a transition pH ≈ 8 [13,15].…”

Section: Experimental Materials and Hydrogel Synthesismentioning

confidence: 99%

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Separation of the effects of pH and polymer concentration on the swelling pressure and elastic modulus of a pH-responsive hydrogel

Horkay

Han

et al. 2006

Polymer

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The elastic shear modulus G and swelling pressure ω are studied for a basic, pH-responsive hydrogel synthesized by crosslinking copolymerization of co-monomers hydroxypropyl methacrylate and N,N-dimethylaminoethyl methacrylate with crosslinker tetraethylene glycol dimethacrylate. Under normal conditions of use as a "smart" material, hydrogel swelling ratio Q and pH vary simultaneously, but here G and ω values are presented as a function of pH with Q held constant and vice-versa. At fixed pH, G decreases with increase in Q in a power law dependence, as predicted by the FloryRehner model. However, at fixed Q, G increases with decrease in pH (i.e, increase in degree of ionization). The pH effect is more pronounced than the volume effect, thus the hydrogel stiffens as it swells in response to pH change. At high pH, ω values of the uncharged hydrogel obey the FloryRehner model, whereas explicit ionic contributions can be identified at lower pH values.

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Section: Experimental Materials and Hydrogel Synthesismentioning

confidence: 99%

“…The monomer DMA contains a pH-sensitive tertiary amine, TEGDMA is a crosslinker, and HPMA was included to obtain a transition pH ≈ 8 [13,15]. Hydrogels containing HPMA/DMA/TEGDMA at a nominal mole ratio of 70/30/02 were prepared by free radical crosslinking copolymerization as in Reference 15.…”

Section: Experimental Materials and Hydrogel Synthesismentioning

confidence: 99%

Separation of the effects of pH and polymer concentration on the swelling pressure and elastic modulus of a pH-responsive hydrogel

Horkay

Han

et al. 2006

Polymer

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“…HPMA monomers prove for the adjustment of the volume phase transition of the hydrogel to the physiological pH range (Jung et al, 2000). TEGDMA acts as a cross-linker, whereas EG is the solvent.…”

Section: Standard Preparation Procedures Of Ph-sensitive Hydrogelsmentioning

confidence: 99%

“…On the other hand, the enzymatic reaction causes pH changes due to the production of gluconic acid. In particular, an increase of the glucose concentration causes a lowering in pH value (Jung et al, 2000). Hence, a GluOx-loaded pH-sensitive hydrogel changes its swelling state dependent on the surrounding glucose concentration.…”

Section: Introductionmentioning

confidence: 99%

Biochemical piezoresistive sensors based on hydrogels for biotechnology and medical applications

Schmidt

Jorsch

Guenther

et al. 2016

J. Sens. Sens. Syst.

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Abstract. Many conventional analysis techniques achieve a high-detection sensitivity; however, they are equipment or time expensive due to a multi-step procedure. Sensor concepts, introduced in this work, using piezoresistive pressure sensor chips with integrated analyte-sensitive hydrogels enable inexpensive and robust biochemical sensors, which are miniaturized and in-line capable. For these sensor setups, it is important to optimize current established analyte-sensitive, reversible and biocompatible hydrogels for pH and glucose monitoring of chemical and biochemical processes. Therefore, low-viscous monomer mixtures based on hydroxypropyl methacrylate (HPMA), 2-(dimethylamino)ethyl methacrylate (DMAEMA), tetraethylene glycol dimethacrylate (TEGDMA) and ethylene glycol (EG) were prepared in molar ratios of 70/30/01/20, 60/40/01/20 and 60/40/02/20, respectively. Redox-polymerization of these pre-gel solutions were realized with N,N,N ,Ntetramethylethylenediamine and ammonium persulfate. The reversible pH-sensitive swelling behavior of hydrogels with compositions were compared. By using the photoinitiator 2-hydroxy-4 -(2-hydroxyethoxy)-2-methylpropiophenone, the free radical photopolymerization could be implemented leading to an increase of the swelling degree (SG). Glucose-sensitive hydrogels were prepared via immobilization of glucose oxidase in HPMA-DMAEMA-TEGDMA-EG hydrogel discs. These showed increasing swelling degrees with higher glucose concentrations in aqueous media and a reversible swelling behavior. The synthesized hydrogels were integrated in different piezoresistive sensors of different designs. The pH-depending course of the output voltage of a dip sensor with photopolymerized 60/40/02/20 hydrogel was studied in detail. Besides the usage of a dip sensor, two implantable, parylene C-coated setups are presented. The implantable sensor with long isolated gold bond wires was proved to be functional even after storage in aqueous media for several days.

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“…Micro-fabricated sensors can provide a continuous monitoring and on-line control of analyte concentrations in ambient aqueous solutions. The chemical sensor containing a special biocompatible polymer (hydrogel) with a sharp volume phase transition in the neutral physiological pH range near 7.4 can detect a specific analyte, for example, carbon dioxide (Herber et al 2004) or glucose (Jung et al 2000). The hydrogel-based biochemical sensors are useful for the diagnosis and monitoring of diabetes and of pulmonary disease.…”

Section: Introductionmentioning

confidence: 99%

Piezoresistive biochemical sensors based on hydrogels

2009

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Already 8 years ago, the usage of piezoresistive sensors for chemical measurands was proposed at the Solid State Electronics Laboratory of the Dresden University of Technology. Adding functionalised polymer coating which shows swelling due to chemical or biological values leads to a similar deflection of the thin silicon bending plate like for pressure sensors. The application of ''stimuli-responsive'' or ''smart'' cross-linked gels in chemical sensors is based on their ability to a phase transition under the influence of external excitations (pH, concentration of additives in water, temperature). Combining a ''smart'' hydrogel and a micro fabricated pressure sensor chip allows to continuously monitor the analyte-dependent swelling of a hydrogel and hence the analyte concentration in ambient aqueous solutions. The sensitivity of hydrogels with regard to the concentration of such additives as H ? -ions (pH sensor), transition-metal ions, salts, organic solvents and proteins in water was investigated.

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Catalase Effects on Glucose-Sensitive Hydrogels

Cited by 50 publications

References 42 publications

Separation of the effects of pH and polymer concentration on the swelling pressure and elastic modulus of a pH-responsive hydrogel

Separation of the effects of pH and polymer concentration on the swelling pressure and elastic modulus of a pH-responsive hydrogel

Biochemical piezoresistive sensors based on hydrogels for biotechnology and medical applications

Piezoresistive biochemical sensors based on hydrogels

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