Catalase Is Encoded by a Multigene Family in Arabidopsis thaliana (L.) Heynh

Frugoli, Julia; Zhong, Hai Hong; Nuccio, Michael L.; McCourt, Peter; McPeek, Mark A.; Thomas, Terry L.; McClung, C. Robertson

doi:10.1104/pp.112.1.327

Cited by 250 publications

(182 citation statements)

References 46 publications

Supporting

Mentioning

155

Contrasting

Unclassified

Order By: Relevance

“…This effect was dependent on the strength of rolB expression. These genes were previously shown to be the main catalase genes participating in ROS detoxification in Arabidopsis (Frugoli et al, 1996) and R. cordifolia .…”

Section: Top)mentioning

confidence: 95%

TherolBGene Suppresses Reactive Oxygen Species in Transformed Plant Cells through the Sustained Activation of Antioxidant Defense

et al. 2012

View full text Add to dashboard Cite

The rolB (for rooting locus of Agrobacterium rhizogenes) oncogene has previously been identified as a key player in the formation of hairy roots during the plant-A. rhizogenes interaction. In this study, using single-cell assays based on confocal microscopy, we demonstrated reduced levels of reactive oxygen species (ROS) in rolB-expressing Rubia cordifolia, Panax ginseng, and Arabidopsis (Arabidopsis thaliana) cells. The expression of rolB was sufficient to inhibit excessive elevations of ROS induced by paraquat, menadione, and light stress and prevent cell death induced by chronic oxidative stress. In rolB-expressing cells, we detected the enhanced expression of antioxidant genes encoding cytosolic ascorbate peroxidase, catalase, and superoxide dismutase. We conclude that, similar to pathogenic determinants in other pathogenic bacteria, rolB suppresses ROS and plays a role not only in cell differentiation but also in ROS metabolism.

show abstract

Section: Top)mentioning

confidence: 95%

TherolBGene Suppresses Reactive Oxygen Species in Transformed Plant Cells through the Sustained Activation of Antioxidant Defense

et al. 2012

View full text Add to dashboard Cite

show abstract

“…gene targeted by the Y 98 , Y 250 , and Y 500 constructs is part of a gene family (CAT1, CAT2, and CAT3), with a nucleotide and amino acid sequence identity of 70% to 72% and 75% to 84%, respectively (Frugoli et al, 1996). The CAT2 and CAT3 genes are highly expressed in leaves.…”

Section: The Efficiency Of Gus Silencing Is Correlated With the Amounmentioning

confidence: 99%

The Frequency and Efficiency of Endogene Suppression by Transitive Silencing Signals Is Influenced by the Length of Sequence Homology

et al. 2006

View full text Add to dashboard Cite

Transitivity, the spread of RNA silencing along primary target sequences, leads to the degradation of secondary targets that have no sequence homology to the initial silencing trigger. We demonstrate that increasing the distance between direct and adjacent target sequences in a transgenic primary target delays the onset of silencing of a secondary target gene. Silencing can spread in a 3# to 5# direction over a distance of at least 500 nucleotides (nt), but this requires consistently more time compared to a distance of 98 nt or 250 nt. The efficiency and frequency of transitive silencing of an endogene depends on the length of its sequence homology with the primary target. With a length of 500 nt, efficient silencing can eventually be established in all plants, whereas lengths of 250 nt and 98 nt homology result in less efficient and less frequent suppression. These results suggest that amplification of secondary small interfering RNAs (siRNAs) is a time-requiring process that gradually expands the population of siRNAs until a steady-state level is reached. Moreover, the length of the sequence homology in the primary target providing secondary siRNAs determines whether this steady-state level readily exceeds the threshold necessary for efficient silencing.

show abstract

“…If introns are lost through gene conversion by RT-mRNAs, adjacent introns may sometimes be lost in concert when a gene conversion event spans multiple intron positions (14,21,22). For each lineage, we compared the pattern of intron loss with the expectation assuming independent intron loss, and found a signal of concerted loss of adjacent introns for diptera and Saccharomyces pombe, but not C. elegans, evidence that the 5Ј bias is due to RT-mRNAmediated intron loss.…”

mentioning

confidence: 99%

The pattern of intron loss

Roy

Gilbert

2005

Proc. Natl. Acad. Sci. U.S.A.

121

117

View full text Add to dashboard Cite

We studied intron loss in 684 groups of orthologous genes from seven fully sequenced eukaryotic genomes. We found that introns closer to the 3 ends of genes are preferentially lost, as predicted if introns are lost through gene conversion with a reverse transcriptase product of a spliced mRNA. Adjacent introns tend to be lost in concert, as expected if such events span multiple intron positions. Directly contrary to the expectations of some, introns that do not interrupt codons (phase zero) are more, not less, likely to be lost, an intriguing and previously unappreciated result. Adjacent introns with matching phases are not more likely to be retained, as would be expected if they enjoyed a relative selective advantage. The findings of 3 and phase zero intron loss biases are in direct contradiction to an extremely recent study of fungi intron evolution. All patterns are less pronounced in the lineage leading to Caenorhabditis elegans, suggesting that the process of intron loss may be qualitatively different in nematodes. Our results support a reverse transcriptase-mediated model of intron loss.evolution ͉ genome evolution T wo generalities of the intron-exon structure of eukaryotic genes remain unexplained. First, introns in intron-sparse species or in intron-sparse genes cluster near the 5Ј ends of genes (1, 2). Second, intron positions within codons are doubly biased: introns tend to lie between the third base of one codon and the first base of the subsequent codon (phase zero) rather than between the first and second (phase one) or second and third (phase two) bases of a codon; and adjacent introns tend to be of the same phase (3-9).The 5Ј skew could be due to mutation-biased intron loss, selection-biased intron loss, or biased intron gain. If intron loss proceeds by means of gene conversion of the genomic copy of a gene by the reverse transcriptase product of a spliced transcript (RT-mRNAs) (10-15), the 3Ј bias of RT products (12) could cause a higher rate of loss for 3Ј introns (1, 2). Alternatively, possible preferential retention of 5Ј introns could reflect their greater selective importance, possibly due to a greater concentration of regulatory elements (reviewed in ref. 16). Finally, intron gain could favor 5Ј ends of genes for some unappreciated reason.We analyzed intron losses in 684 groups of orthologous genes from seven eukaryotic species, previously analyzed by Rogozin et al. (17). Fig. 1 shows the most likely phylogeny for the species (18). Results calculated assuming the alternative coelomata grouping (19,20) are similar and provided in Tables 3 and 4 and Figs. 6-9, which are published as supporting information on the PNAS web site. For each lineage, we defined introns known to be ancestral to the lineage (KAL) based on presence in both the sister group of the lineage and an outgroup. For example, introns present in a dipteran (Drosophila melanogaster and Anopheles gambiae) or Caenorhabditis elegans as well as a non-animal are KAL for the lineage leading to Homo sapiens. We found that 3Ј KAL intr...

show abstract

Catalase Is Encoded by a Multigene Family in Arabidopsis thaliana (L.) Heynh

Cited by 250 publications

References 46 publications

TherolBGene Suppresses Reactive Oxygen Species in Transformed Plant Cells through the Sustained Activation of Antioxidant Defense

TherolBGene Suppresses Reactive Oxygen Species in Transformed Plant Cells through the Sustained Activation of Antioxidant Defense

The Frequency and Efficiency of Endogene Suppression by Transitive Silencing Signals Is Influenced by the Length of Sequence Homology

The pattern of intron loss

Contact Info

Product

Resources

About