Catalase Prevents Lipid Peroxidation and Enhances Survival of Caprine Preantral Follicles Cryopreserved in a 1,2-Propanediol-Freezing Medium

Luz, Hiédely Kenia Machado; Santos, Regiane R.; Wanderley, Livia Schell; Faustino, Luciana Rocha; Silva, Cleidson M.G.; Carvalho, Adonias Almeida; Campello, C.C.; Santos, Francielli Weber; Figueiredo, J.R.; Rodrigues, Ana Paula Ribeiro

doi:10.1089/bio.2011.0046

Cited by 18 publications

(9 citation statements)

References 25 publications

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“…After the ovarian tissues had been homogenized, the supernatant was incubated for 3 h at 95°C in a 1.5 mL of 20% acetic acid solution containing 0.27 M HCl and 1.5 L of 0.8% thiobarbituric acid (TBA) solution. Absorbance was calculated using a plate reader at 532 nm to determine the MDA levels (Luz et al 2012).…”

Section: Measurement Of Lipid Peroxidationmentioning

confidence: 99%

Ovarian tissue culture in the presence of VEGF and fetuin stimulates follicle growth and steroidogenesis

Asadi

Najafi

Moeini

et al. 2017

Journal of Endocrinology

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Ovarian tissue cryopreservation together with follicle culture provides a promising technique for fertility preservation in cancer patients. The study aimed to evaluate follicle parameters in a culture medium supplemented with VEGFA165 and/or fetuin. Vitrified-warmed ovarian cortical pieces were divided randomly into four culture groups consisting of basic culture medium (control), and the basic culture medium supplemented with VEGFA165, fetuin or both. After six days of culture, we evaluated the following: percentage of resting, primary and secondary growing follicles; survival rate; steroid hormones production; levels of reactive oxygen species, lipid peroxidation and total antioxidant capacity; and developmental and antioxidant gene expression. The addition of VEGFA165 alone or in combination with fetuin to the culture medium caused resting follicle activation and increased the number of growing follicles. In the VEGFA165 group, we found a significant increase in the concentrations of 17β-estradiol at day 6 and progesterone from 4th day of the culture period. In the VEGFA165 + fetuin group, the concentration of 17β-estradiol rose at day 4 of the culture period. The levels of BMP15, GDF9 and INHB mRNAs were increased in all treated groups. In the fetuin and fetuin + VEGFA165 groups, we observed a high level of total antioxidant capacity and expression of SOD1 and CAT genes, low reactive oxygen species and lipid peroxidation levels and increased number of viable follicles. In conclusion, the present study provides useful evidence that supplementation of culture medium with VEGFA165 + fetuin leads to primordial follicle activation and development and increased percentage of healthy secondary growing follicles.

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Section: Measurement Of Lipid Peroxidationmentioning

confidence: 99%

Ovarian tissue culture in the presence of VEGF and fetuin stimulates follicle growth and steroidogenesis

Asadi

Najafi

Moeini

et al. 2017

Journal of Endocrinology

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“…27 Desta forma, na tentativa de reduzir a formação de ERO S in vitro, alguns pesquisadores têm utilizado agentes antioxidantes na solução de criopreservação. [28][29][30][31]…”

Section: áCido Alfa Lipoico (Ala)unclassified

“…13 Considerando a presença e importância de agentes antioxidantes no sistema reprodutor, essas substâncias também são frequentemente utilizadas em técnicas de reprodução assistida, como por exemplo, a criopreservação tanto de sêmen 35 como de oócitos 36 ou mesmo de tecido ovariano. [29][30][31] É importante destacar que as EROs são produzidas nas mitocôndrias, por tanto essa organela merece uma grande A peroxidação lipídica ou lipoperoxidação é a incorporação de um oxigênio molecular (radical livre) sobre os ácidos graxos da membrana celular, levando à destruição de sua estrutura, perda das trocas metabólicas e, em última condição, à morte celular. A peroxidação lipídica parece ser o principal dano causado às membranas, 46 e evidências sugerem que a CAT é capaz de reduzir esses danos.…”

Section: Antioxidantesunclassified

“…A peroxidação lipídica parece ser o principal dano causado às membranas, 46 e evidências sugerem que a CAT é capaz de reduzir esses danos. 29,30 Utilização da CAT na criopreservação de oócitos e folículos pré-antrais A Catalse foi utilizada em combinação com superóxido desmutase (SOD) na solução de congelação lenta de óocitos de camundongos, e demonstrou-se que essa associação pode proteger contra um desequilíbrio no sistema de reduçãooxidação. 36 Durante o congelamento lento de tecido ovariano caprino, a suplementação de CAT na concentração de 20UI/ml na solução de criopreservação conservou a viabilidade folicular e reduziu a peroxidação lipídica em relação ao controle.…”

Section: Antioxidantesunclassified

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Importância da utilização de ácido alfa lipóico (ALA) e catalase (CAT) no processo de criopreservação de folículos ovarianos pré-antrais, visando reduzir os danos causados pelo estresse oxidativo

et al. 2020

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A criopreservação de folículos ovarianos pré-antrais é uma perspectiva promissora para a preservação da fertilidade e desenvolvimento de banco de oócitos ou em caso de patologias e terapêuticas que possam comprometer as reservas foliculares. A grande vantagem da criopreservação do tecido ovariano é que independe da idade e fase do ciclo menstrual/estral, além de envolver menos questões éticas e sociais. No entanto, como qualquer exposição de um material biológico a temperaturas extremamente baixas, a criopreservação pode levar a um desequilíbrio na produção de espécies reativas de oxigênio (EROs), podendo causar danos celulares. In vivo, as EROS são equilibradas pelo sistema de defesa de antioxidantes, porém, in vitro, a falta do sistema fisiológico, pode levar ao estresse oxidativo. Portanto, antioxidantes vêm sendo utilizados antes ou após a criopreservação, os quais pode-se destacar o ácido alfa lipóico ou ácido tióctico (ALA) e catalase (CAT). Por tanto, o objetivo desse artigo é auxiliar no entendimento da relevância da criopreservação do tecido ovariano para a preservação da fertilidade das fêmeas, bem como o controle na produção de EROS durante o processo, e a necessidade da suplementação de antioxidantes como o ALA e a CAT.

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“…In earlier studies, ascorbic acid was used as an antioxidant agent but did not improve ovine follicular viability (Melo et al 2011). Additional studies showed that the supplementation of freezing medium with taurine and L-glutamine (Sanfilippo et al 2013) and with catalase or Trolox (Luz et al 2012) preserved the pre-antral follicles morphology of human and caprine ovarian tissue, respectively. From these compounds, 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid or Trolox appears as a promising candidate to improve freezing because, as a vitamin E analog, it might reduce oxidation in cell membrane lipids and is also a membrane-stabilizing agent (Bradford et al 2003).…”

Section: Introductionmentioning

confidence: 99%

Vitamin E-analog Trolox prevents endoplasmic reticulum stress in frozen-thawed ovarian tissue of capuchin monkey (Sapajus apella)

Brito

Scalercio

et al. 2013

Cell Tissue Res

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Ovarian fragments were exposed to 0.5 M sucrose and 1 M ethylene glycol (freezing solution; FS) with or without selenium or Trolox. Histological and ultrastructural analyses showed that the percentages of normal follicles in control tissue and in tissue after exposure to FS+50 μM Trolox were similar. Trolox prevented endoplasmic reticulum (ER)-related vacuolization, which is commonly observed in oocytes and stromal tissue after exposure to FS. From the evaluated stress markers, superoxide dismutase 1 (SOD1) was up-regulated in ovarian tissue exposed to FS+10 ng/ml selenium. Ovarian fragments were subsequently frozenthawed in the presence of FS with or without 50 μM Trolox, followed by in vitro culture (IVC). Antioxidant capacity in ovarian fragments decreased after freeze-thawing in Troloxfree FS compared with FS+50 μM Trolox. Although freezing itself minimized the percentage of viable follicles in each solution, Trolox supplementation resulted in higher rates of viable follicles (67 %), even after IVC (61 %). Furthermore, stress markers SOD1 and ERp29 were up-regulated in ovarian tissue frozen-thawed in Trolox-free medium. Relative mRNA expression of growth factors markers was evaluated after freeze-thawing followed by IVC. BMP4, BMP5, CTGF, GDF9 and KL were down-regulated independently of the presence of Trolox in FS but down-regulation was less pronounced in the presence of Trolox. Thus, medium supplementation with 50 μM Trolox prevents ER stress and, consequently, protects ovarian tissue from ER-derived cytoplasmic vacuolization. ERp29 but not ERp60, appears to be a key marker linking stress caused by freezing-thawing and cell vacuolization.

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Catalase Prevents Lipid Peroxidation and Enhances Survival of Caprine Preantral Follicles Cryopreserved in a 1,2-Propanediol-Freezing Medium

Cited by 18 publications

References 25 publications

Ovarian tissue culture in the presence of VEGF and fetuin stimulates follicle growth and steroidogenesis

Ovarian tissue culture in the presence of VEGF and fetuin stimulates follicle growth and steroidogenesis

Importância da utilização de ácido alfa lipóico (ALA) e catalase (CAT) no processo de criopreservação de folículos ovarianos pré-antrais, visando reduzir os danos causados pelo estresse oxidativo

Vitamin E-analog Trolox prevents endoplasmic reticulum stress in frozen-thawed ovarian tissue of capuchin monkey (Sapajus apella)

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