Catalases CAT1 and CAT3 Are not Key Enzymes in Alleviating Gamma Irradiation-Induced DNA Damage, H2O2Accumulation, or Lipid Peroxidation inArabidopsis thaliana

Sultana, Amena; Minami, Ikuko; Matsushima, Daiki; Issak, Mohammad; Nakamura, Yoshimasa; Todoriki, Setsuko; Murata, Yoshiyuki

doi:10.1271/bbb.130336

Cited by 5 publications

(3 citation statements)

References 13 publications

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“…MDA content was detected using Lipid Peroxidation (MDA) Assay Kit (Sigma-Aldrich). ADP and ATP levels were detected using a EnzyLight™ ATP assay (BioAssay Systems).The levels of AsA, GSSG and GSH were detected as described previously 4,64 .…”

Section: Methodsmentioning

confidence: 99%

PtomtAPX, a mitochondrial ascorbate peroxidase, plays an important role in maintaining the redox balance of Populus tomentosa Carr

Yin

Zhang

Liu

et al. 2019

Sci Rep

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Plant mitochondria are important energy-producing structure and ROS are generated as byproducts. APX is one enzyme of the AsA-GSH cycle to reduces H2O2 to water. We identified both PtomtAPX and PtosAPX are located in mitochondria of Populus tomentosa Carr. PtomtAPX is specifically targeted to mitochondria, while PtosAPX is dual targeted to both chloroplast and mitochondria. The expression of PtomtAPX in mitochondria was 60-fold that of PtosAPX by ELISA and qPCR analysis. Under high light stress, the expression levels of PtosAPX increased, while that of PtomtAPX only slightly changed. Compared to the WT, the antisense transgenic PtomtAPX cell lines showed slowed growth, smaller cells impaired mitochondria in MS medium under normal growth. RNA-seq results showed 3121 genes significantly altered expression in the antisense cells, and most of them are important for mitochondrial function, particularly in oxidative phosphorylation. Our findings demonstrates a mitochondrial location for one APX isoform, and provide valuable insight into the mechanism which ROS balance is modulated by AsA-GSH cycle in mitochondria.

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Section: Methodsmentioning

confidence: 99%

PtomtAPX, a mitochondrial ascorbate peroxidase, plays an important role in maintaining the redox balance of Populus tomentosa Carr

Yin

Zhang

Liu

et al. 2019

Sci Rep

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“…We recently have presented that there was no significant differences in susceptibility to gamma irradiation between Arabidopsis wild type and other catalase deficient mutants, cat3 and cat1 cat3. 11) Hence, catalases may not be key enzymes to protect cellular damage induced by gamma irradiation in Arabidopsis.…”

Section: Resultsmentioning

confidence: 99%

Catalase, CAT2, is not Involved in Mitigation of Gamma Irradiation-induced H2O2 Accumulation or Lipid Peroxidation in Arabidopsis thaliana

Sultana¹,

Minami²,

Matsushima³

et al. 2013

FOOD IRRADIATION, JAPAN

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“…Fifteen genes are associated with ROS scavenging, and 7 and 8 genes are involved in the phytohormones ABA and ethylene, respectively (Table S3). As shown in Figure 7, there was a significant increase in the expression of CPK5 [20][21][22] and CPK28 [23], GST [24], GPX6 [25], APX1 [26], CAT1 [27], CAT2 [28], CAT3 [29], PYL9 [30], PP2CA [31,32], ACS6 [33], and ERF1A [34]. In RbohD1-related, Ros scavenging, and ABA-related genes, except for a few genes, such as CPK4 [35], GPK6 [36], APX6 [37], etc., most of them showed an increasing trend.…”

Section: Comparison Of the Expression Of Rbohd1-related Genes Between...mentioning

confidence: 91%

RsRbohD1 Plays a Significant Role in ROS Production during Radish Pithiness Development

Gong,

Wang,

Fan

et al. 2024

Plants

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Pithiness is one of the physiological diseases of radishes, which is accompanied by the accumulation of reactive oxygen species (ROS) during the sponging of parenchyma tissue in the fleshy roots. A respiratory burst oxidase homolog (Rboh, also known as NADPH oxidase) is a key enzyme that catalyzes the production of ROS in plants. To understand the role of Rboh genes in radish pithiness, herein, 10 RsRboh gene families were identified in the genome of Raphanus sativus using Blastp and Hmmer searching methods and were subjected to basic functional analyses such as phylogenetic tree construction, chromosomal localization, conserved structural domain analysis, and promoter element prediction. The expression profiles of RsRbohs in five stages (Pithiness grade = 0, 1, 2, 3, 4, respectively) of radish pithiness were analyzed. The results showed that 10 RsRbohs expressed different levels during the development of radish pithiness. Except for RsRbohB and RsRbohE, the expression of other members increased and reached the peak at the P2 (Pithiness grade = 2) stage, among which RsRbohD1 showed the highest transcripts. Then, the expression of 40 genes related to RsRbohD1 and pithiness were analyzed. These results can provide a theoretical basis for improving pithiness tolerance in radishes.

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Catalases CAT1 and CAT3 Are not Key Enzymes in Alleviating Gamma Irradiation-Induced DNA Damage, H₂O₂Accumulation, or Lipid Peroxidation inArabidopsis thaliana

Cited by 5 publications

References 13 publications

PtomtAPX, a mitochondrial ascorbate peroxidase, plays an important role in maintaining the redox balance of Populus tomentosa Carr

PtomtAPX, a mitochondrial ascorbate peroxidase, plays an important role in maintaining the redox balance of Populus tomentosa Carr

Catalase, CAT2, is not Involved in Mitigation of Gamma Irradiation-induced H2O2 Accumulation or Lipid Peroxidation in Arabidopsis thaliana

RsRbohD1 Plays a Significant Role in ROS Production during Radish Pithiness Development

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