Catalogue of soluble proteins in the human vitreous humor: comparison between diabetic retinopathy and macular hole

Nakanishi, Toyofumi; Koyama, Reiko; Ikeda, Tsunehiko; Shimizu, Akira

doi:10.1016/s1570-0232(02)00078-8

Cited by 74 publications

(84 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Moreover, 240 vitreous proteins, which have not been reported previously in vitreous [22][23][24][25][35][36][37], were identified during the present study, these include, hepatocyte growth factor activator, kallistatin, thioredoxin, von Willebrand factor (vWF), Wnt inhibitory factor, chromogranin and secreted frizzled-related protein (see Table S7 in Supporting Information for a listing). Moreover, some of these Figure 3.…”

Section: Identified Protein Lists From Lc-maldi-ms/ms and Lc-esi-ms/msmentioning

confidence: 59%

See 1 more Smart Citation

Profiling of vitreous proteomes from proliferative diabetic retinopathy and nondiabetic patients

Kim

et al. 2007

Proteomics

133

View full text Add to dashboard Cite

Diabetes can lead to serious microvascular complications like proliferative diabetic retinopathy (PDR), which is the leading cause of blindness in adults. The proteomic changes that occur during PDR cannot be measured in the human retina for ethical reasons, but could be reflected by proteomic changes in vitreous humor. Thus, we considered that comparisons between the proteome profiles of the vitreous humors of PDR and nondiabetic controls could lead to the discovery of novel pathogenic proteins and clinical biomarkers. In this study, the authors used several proteomic methods to comprehensively examine vitreous humor proteomes of PDR patients and nondiabetic controls. These methods included immunoaffinity subtraction (IS)/2-DE/ MALDI-MS, nano-LC-MALDI-MS/MS, and nano-LC-ESI-MS/MS. The identified proteins were subjected to the Trans-Proteomic Pipeline validation process. Resultantly, 531 proteins were identified, i.e., 415 and 346 proteins were identified in PDR and nondiabetic control vitreous humor samples, respectively, and of these 531 proteins, 240 were identified for the first time in this study. The PDR vitreous proteome was also found to contain many proteins possibly involved in the pathogenesis of PDR. The proteins described provide the most comprehensive proteome listing in the vitreous humor samples of PDR and nondiabetic control patients.

show abstract

Section: Identified Protein Lists From Lc-maldi-ms/ms and Lc-esi-ms/msmentioning

confidence: 59%

“…Recent advances in 2-DE and MS have allowed further exploration and acquisition of vitreous protein profiles [22][23][24]. In our previous study, by using both 2-DE and MALDI-TOF MS, we constructed PDR vitreous protein profiles and identified eight proteins that are possibly involved in the pathogenesis of PDR [25].…”

Section: Introductionmentioning

confidence: 99%

Profiling of vitreous proteomes from proliferative diabetic retinopathy and nondiabetic patients

Kim

et al. 2007

Proteomics

133

View full text Add to dashboard Cite

show abstract

“…However, few proteome analyses in human vitreous fluid have been performed in the setting of diabetic eye disease [7][8][9][10][11], and no previous quantitative proteomic comparison has been reported. The aim of the present study was to compare the protein profile of human vitreous fluid from diabetic patients with PDR with that of vitreous fluid obtained from nondiabetic patients with idiopathic macular holes (MH), a condition in which, in contrast to PDR, the retina is not affected by neovascularisation.…”

Section: Introductionmentioning

confidence: 99%

Proteomic analysis of human vitreous fluid by fluorescence-based difference gel electrophoresis (DIGE): a new strategy for identifying potential candidates in the pathogenesis of proliferative diabetic retinopathy

Garcia-Ramírez

Canals

Hernández³

et al. 2007

Diabetologia

151

135

View full text Add to dashboard Cite

Aims/hypothesis The aim of this study was to compare the protein profile of vitreous fluid from diabetic patients with proliferative diabetic retinopathy (PDR) with that from nondiabetic patients with idiopathic macular holes (MH). The mRNA of proteins differentially produced was also assessed in the retinas from diabetic and non-diabetic donors. Materials and methods Vitreous humour from type 1 diabetic patients with PDR (n=8) and from non-diabetic patients with MH (n=10) closely matched in terms of age were studied. The comparative proteomic analysis was performed using fluorescence-based difference gel electrophoresis (DIGE). Differentially produced proteins (abundance ratio >1.4, p<0.05) were identified by mass spectrometry. Expressions of mRNA were measured by real-time RT-PCR in retinas from ten human eyes obtained at post-mortem (five eyes from diabetic subjects and five eyes from non-diabetic subjects). Results Eight proteins were highly produced in PDR patients in comparison with non-diabetic subjects: zinc-α 2 -glycoprotein (ZAG), apolipoprotein (apo) A1, apoH, fibrinogen A, and the complement factors C3, C4b, C9 and factor B). We found three proteins that were underproduced in PDR subjects: pigment epithelial derived factor (PEDF), interstitial retinol-binding protein (IRBP) and inter-α-trypsin inhibitor heavy chain (ITIH2). There was no overlap in the vitreous levels of the above-mentioned proteins between PDR patients and non-diabetic control subjects. The differential production of ZAG, C3, factor B, PEDF and IRBP was further confirmed by western blot, and was in agreement with mRNA levels detected in the retina. Conclusions/interpretation Proteomic analysis by DIGE, which permits an accurate quantitative comparison, was useful in identifying new potential candidates involved in the pathogenesis of PDR.

show abstract

“…To date, biochemical studies of human diabetic retinopathy have been mostly limited to analysis of the vitreous [22,[24][25][26]. These analyses have revealed several pathways and factors that provided insight into diabetes-related ocular alterations.…”

Section: Resultsmentioning

confidence: 99%

“…VEGF-A) and anti-angiogenic (i.e. sol flt-1 receptor, PEDF) factors to be present in proliferative diabetic retinopathy [25,26]. Despite their contributions to an understanding of diabetic retinopathy, several limitations are associated with these studies.…”

Section: Resultsmentioning

confidence: 99%

Human retinal pigment epithelium proteome changes in early diabetes

et al. 2008

View full text Add to dashboard Cite

Aims/hypothesis Diabetic retinopathy is the most common complication of diabetes and a leading cause of blindness among working-age adults. Anatomical and functional changes occur in the retina and retinal pigment epithelium (RPE) prior to clinical symptoms of the disease. However, the molecular mechanisms responsible for these early changes, particularly in the RPE, remain unclear. To begin defining the molecular changes associated with pre-retinopathic diabetes, we conducted a comparative proteomics study of human donor RPE. Methods The RPE was dissected from diabetic human donor eyes with no clinically apparent diabetic retinopathy (n=6) and from eyes of age-matched control donors (n= 17). Soluble proteins were separated based upon their mass and charge using two-dimensional (2-D) gel electrophoresis. Protein spots were visualised with a fluorescent dye and spot densities were compared between diabetic and control gels. Proteins from spots with significant disease-related changes in density were identified using mass spectrometry.Results Analysis of 325 spots on 2-D gels identified 31 spots that were either up-or downregulated relative to those from age-matched control donors. The protein identity of 18 spots was determined by mass spectrometry. A majority of altered proteins belonged to two major functional groups, metabolism and chaperones, while other affected categories included protein degradation, synthesis and transport, oxidoreductases, cytoskeletal structure and retinoid metabolism. Conclusions/interpretation Changes identified in the RPE proteome of pre-retinopathic diabetic donor eyes compared with age-matched controls suggest specific cellular alterations that may contribute to diabetic retinopathy. Defining the pre-retinopathic changes affecting the RPE could provide important insight into the molecular events that lead to this disease.

show abstract

Catalogue of soluble proteins in the human vitreous humor: comparison between diabetic retinopathy and macular hole

Cited by 74 publications

References 12 publications

Profiling of vitreous proteomes from proliferative diabetic retinopathy and nondiabetic patients

Profiling of vitreous proteomes from proliferative diabetic retinopathy and nondiabetic patients

Proteomic analysis of human vitreous fluid by fluorescence-based difference gel electrophoresis (DIGE): a new strategy for identifying potential candidates in the pathogenesis of proliferative diabetic retinopathy

Human retinal pigment epithelium proteome changes in early diabetes

Contact Info

Product

Resources

About