Catalytic biofilm formation by Shewanella oneidensis MR-1 and anode characterization by expanded uncertainty

Surface Modification for Enhanced Biofilm Formation and Electron Transport in Shewanella Anodes

“…[30][31][32] Previous studies have shown that, after 24 h of polarization, when an S. oneidensis biofilm is covering the electrode surface, the number of cells attached to the surface was defined mostly by the ECSA and the expanded uncertainty of the anode's response decreased significantly when the current was normalized to that parameter. 27 At the same time, our group has also demonstrated the importance of the hydrophilicity and surface morphology on bacteria attachment and the dynamics of biofilm formation determining the start up time of MFCs. 16 Therefore in this study the important parameters related to the properties of the electrode material have been examined.…”

“…The electrochemical cell was purged with nitrogen and sealed with Parafilm (Sigma) throughout the experiments to maintain micro-aerobic conditions. 27 Determination of riboflavin content.-Cyclic voltammetry measurements of the S. oneidensis anodes after 24 hours of potentiostatic polarization were carried out at various scan rates. A couple of redox peaks associated with the riboflavin oxidation/reduction was observed, and a linear dependence of the peak currents from the scan rate was detected.…”

“…27 Shewanella MR-1 suspended in 50 mM phosphate buffer was introduced in the cells prior to electrochemical experiments. All electrochemical tests were done using a Gamry Reference 600 potentiostat (Gamry Instruments, Warminster, PA).…”

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“…This approach is highly innovative in this field and it relies on statistical analysis and data interpretation. Through evaluation of the expanded uncertainty of microbial fuel cells, the variations in electrodes resistance and mostly difference in their electrochemical accessible surface area were identified as the main parameters causing irreproducibility of MFCs responses [24,25]. The idea of using statistical approaches for analysis of biofuel cells was further enlarged to include Design of Experiments, Principal Component Analysis (PCA) and Canonical Correspondence Analysis [7,[26][27][28][29].…”

Bioelectrocatalysis and More

“…Expanded uncertainty is a new term in the field of biofuel cells, which was recognized as necessary and introduced for the interpretation of results first from microbial fuel cells [24,25]. As for conventional fuel cells, the commonly used electrochemical techniques for MFCs operational characteristics determination are polarization and power curves.…”