Catalytic Decomposition of the Oleaginous Yeast Cutaneotrichosporon Oleaginosus and Subsequent Biocatalytic Conversion of Liberated Free Fatty Acids

Abstract

A single step catalytic cell wall lysis and triglyceride hydrolysis combined with the enzymatic conversion of lipids using the oleaginous yeast Cutaneotrichosporon oleaginosus (ATCC 20509) as a model is described. Catalytic decomposition of yeast cells resulted in hydrolysis of about a third of cellular polysaccharides and all triglycerides. Enzymatic processing of the lipid fraction with an oleate hydratase from Stenotrophomonas maltophilia led to conversion of oleic acid to 10-hydroxystearic acid (10-HSA) (50%) without additional purification. Cell wall polysaccharides were depolymerized by in situ formed amino acids from cell protein fragments. The activity of the in situ generated, free amino acids was higher compared to that of additionally added acids. Studies with the cellobiose and β-(1→3)-glucan indicated that glutamic and aspartic acids, which are the dominant amino acids in yeast cells, are surprisingly more effective in hydrolysis in aqueous phase than sulfuric acid. This points to a concerted mechanism of glycosidic ether bond cleavage catalyzed by amino acids rather than to a pathway catalyzed by hydronium ions. The overall yield of the presented downstream process at 453 K resulted in the release of 80% of total lipids.

Cutaneotrichosporon oleaginosus: A Versatile Whole-Cell Biocatalyst for the Production of Single-Cell Oil from Agro-Industrial Wastes

“…are often associated with humans as hosts and can represent opportunistic pathogens for humans, but among these, some species are studied for important biotechnological applications. In particular, members of the Trichosporonaceae are known to be capable of producing and accumulating large quantities of single-cell oil with respect to their dry biomass [38][39][40][41][42][43][44][45].…”

Mastering targeted genome engineering of GC-rich oleaginous yeast for tailored plant oil alternatives for the food and chemical sector

“…Accordingly, the absence of the two PUFAs (C18:2 and C18:3) in ∆D12 resulted in higher oxidative stability of the oil (Additional File 2 : Table S1). Furthermore, C18:1 can be channelled into the production of other valuable compounds such as 10-(R)-hydroxy stearic acid [ 38 , 39 ] by expressing additional enzymes in the respective strains.…”

Lipase‐mediated plant oil hydrolysis—Toward a quantitative glycerol recovery for the synthesis of pure allyl alcohol and acrylonitrile

“…10-HSA is a drop-in alternative to the 12-HSA, conventionally sourced from castor oil and commonly used in food or pharmaceutical applications as well as in lubricant additives. [3][4][5] Hydroxy fatty acids like 10-HSA can be applied for estolide production, which are i.a., a constituent in biobased lubricant formulations. [6] Further oleic acid can be converted to several other commercially desirable products such as structured lipids or by ozone splitting to polymer intermediates like azelaic acid.…”