Catalytic lectin (leczyme) from bullfrog (Rana catesbeiana) eggs

Nitta, Kazuo; Ozaki, Kouichi; Tsukamoto, Yasuo; Hosono, Masahiro; OgawaKonno, Y; Kawauchi, Hiroaki; Takayanagi, Yoshio; Tsuiki, Shigeru; Hakomori, S

doi:10.3892/ijo.9.1.19

Cited by 14 publications

(14 citation statements)

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“…To determine whether cSBL affects growth stimulatory or inhibitory signals, we compared the intracellular Ca 2+ concentration with protein kinase A, G and C activities. Treatment of P388 cells with cSBL leads to a decrease in the intracellular Ca 2+ concentration and in protein kinase A activity, but increases protein kinase G activity [46]. Treatment of RC-150 cells with cSBL had no effect on any of these parameters.…”

Section: Multi-author Review Articlementioning

confidence: 90%

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Biochemistry of frog ribonucleases

Irie

Nitta

Nonaka

1998

Cellular and Molecular Life Sciences (CMLS)

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Frogs have unique pyrimidine base-specific RNases, with structures similar to those of turtle, iguana and chicken RNases. Among the four frog RNases discussed here, three from Rana pipiens, R. catesbeiana and R. japonica oocyte cells show antitumour activity, and the latter two show lectin activity towards sialic acid-rich glycoproteins. In this review, (i) we compare their unique primary structures with respect to the locations of their disulphide bridges, three-dimensional structure, base specificity and heat stability as compared with RNase A, and (ii) we summarize current knowledge about the mode of action of lectin and the antitumour activities of the three frog RNases.

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Section: Multi-author Review Articlementioning

confidence: 90%

“…showed no RNA degradation even over the course of 24 h [46]. Total RNA extracted from either P388 or RC-150 cells showed equal degradation when treated directly with cSBL.…”

Section: Multi-author Review Articlementioning

confidence: 93%

Biochemistry of frog ribonucleases

Irie

Nitta

Nonaka

1998

Cellular and Molecular Life Sciences (CMLS)

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“…But because CD23 has multifunctional, multistructural characteristics, it is difficult to put it in a typical one-only category. There are many fragmental facts that seem to be related with this CD23 as a lectin, and as a Fce receptor: eggs are also likeky to have animal lectin, as many seeds have plant lectins; sialic acid-binding lectin from bullfrog (Rana catesbeiane) eggs has ribonuclease activity and has been named 'leczyme' by Nitta et al [120]. Other lectins are related with such enzyme activities as protease or lysophospholipase [24]; MBP recognizes abundant mannose on yeast cell surfaces, whereas some lectins recognize abundant LPS on bacterial surfaces or are induced by it [51], and an insect lectin was induced by injury [121]; Kawasaki et al found that MBP activates the complement system [122], whereas it kills cancer cells by an unknown complement-independent manner [123]; recognition of non-self by macrophage, and the recognized molecules transported into cells by endocytosis through macrophage lectins or CD23; recognition of non-self by NK cells with NK receptors through MHC class I molecules and inhibition of killer activity by NKRs; the appearance of immunoglobulins that recognize non-self with high specificity, and some of the lectins were probably used as Fc receptors, as discussed in the review 'Were lectins primitive Fc receptors?'…”

Section: The Mysterious and Attractive Molecule: Cd23mentioning

confidence: 97%

CD23 (the low-affinity IgE receptor) as a C-type lectin: a multidomain and multifunctional molecule

Kijimoto-Ochiai

2002

Cellular and Molecular Life Sciences (CMLS)

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This review, regards the low-affinity receptor CD23 as a C-type lectin and compares it with other C-type lectins and C-type lectin-like receptors. C-type lectins such as the asialoglycoprotein receptor, as well as the dendritic cell immunoreceptor and the dendritic cell-specific intercellular adhesion molecule-3-grabbing non-integrin on dendritic cell lectin, possess amino acid sequences which interact with Ca++ and sugar, and many of them possess an endocytosis signal sequence that includes tyrosine or serine in the cytoplasmic region. In contrast, natural killer receptors lack the Ca++ and sugar-binding amino acids but conserve homologous cysteines in the form of C-type lectin, and possess an immunoreceptor tyrosine-based inhibitory motif in the cytoplasmic region which inhibits killer activity when they recognize the self major histocompatibility (MHC) class I molecule. Since human CD23a form has a similar amino acid sequence, the possibility that this sequence is an endocytosis signal or an ITIM is discussed. The function of the reverse RGD and RGD-binding inhibitory peptide in human CD23 from the point of view of the relation between a C-type lectin and MHC class II molecules is also considered.

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“…The amino acid sequence of SBL shows that it has homology to members of the RNase A superfamily, and it has been revealed that SBL has pyrimidine base-specific ribonuclease activity (14–17). The antitumor effect of SBL was reported using P388 and L1210 murine leukemia cells in vitro and sarcoma 180 cells and Ehrlich and Mep 2 ascites cells in vivo (18–20). We recently reported that SBL had a cytotoxic effect on various leukemia cells including MDR cells and showed that this cytotoxicity was induced through multiple apoptotic signaling pathways (21).…”

Section: Introductionmentioning

confidence: 99%

Downregulation of Hsp70 inhibits apoptosis induced by sialic acid-binding lectin (leczyme)

et al. 2013

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Heat shock proteins (Hsps) are molecular chaperones that maintain homeostasis of organisms. In regards to the Hsps, many studies have investigated the structure, expression, localization and functions of Hsp70 and Hsc70 including expression in the glycosphingolipid-enriched microdomain (GEM) on the cell surface and involvement in cell death. Sialic acid-binding lectin (SBL) isolated from oocytes of Rana catesbeiana is a multifunctional protein which has lectin activity, ribonuclease activity and antitumor activity. SBL has potential as a new type of anticancer drug, since it causes cancer-selective induction of apoptosis by multiple signaling pathways in which RNA is its target; and the participation of the mitochondrial pathway and the endoplasmic reticulum (ER) stress-mediated pathway has been suggested. It has also been suggested that receptor(s) for SBL (SBLR) may exist in the GEM on the cell surface. In the present study, we studied the possible involvement of Hsp70 and Hsc70 in SBL-induced apoptosis. We showed that Hsp70 and Hsc70 were expressed on the P388 cell surface similar to SBLR, and their distribution in cells dramatically changed immediately prior to the execution of apoptosis following stimulation of SBL. Functional study of Hsp70 revealed that decreased expression of Hsp70 diminished the apoptosis induced by SBL. It is suggested that Hsp70 participates in the antitumor effect of SBL.

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Catalytic lectin (leczyme) from bullfrog (Rana catesbeiana) eggs

Cited by 14 publications

References 0 publications

Biochemistry of frog ribonucleases

Biochemistry of frog ribonucleases

CD23 (the low-affinity IgE receptor) as a C-type lectin: a multidomain and multifunctional molecule

Downregulation of Hsp70 inhibits apoptosis induced by sialic acid-binding lectin (leczyme)

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